Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,695,997	C→T	*63* (TAG→TAA)	yhjR ←	DUF2629 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,695,997	0	C	T	100.0%	235.3 / NA	73	*63* (TAG→TAA)	yhjR	DUF2629 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/32);  total (41/32)

CCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTG  >  NC_000913/3695865‑3696131                                                                                                                                     |                                                                                                                                       ccGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACttt                                                                                                                                  >  2:816652/1‑139 (MQ=255)    ggcaggcaGCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCTCTTACTTttgt                                                                                                                               >  2:1048602/1‑139 (MQ=255)                   ccaGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGcc                                                                                                                >  2:991935/1‑139 (MQ=255)                       gACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCa                                                                                                            >  2:532741/1‑139 (MQ=255)                            tttCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATAGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                       <  1:1048602/139‑1 (MQ=255)                              tctcCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCa                                                                                                     <  1:1524171/139‑1 (MQ=255)                                                gACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGc                                                                                   >  2:843481/1‑139 (MQ=255)                                                 aCCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                  >  2:1011271/1‑139 (MQ=255)                                                   cAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCaa                                                                                >  2:1240362/1‑139 (MQ=255)                                                      gCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTg                                                                             >  1:525973/1‑139 (MQ=255)                                                           tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                       <  2:1466111/108‑1 (MQ=255)                                                           tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                       >  1:1466111/1‑108 (MQ=255)                                                           tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                          <  1:1098616/121‑1 (MQ=255)                                                           tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                          >  2:1098616/1‑121 (MQ=255)                                                           tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgc                                                                        >  2:1550093/1‑139 (MQ=255)                                                                cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttg                                                                                                                                <  1:1571090/78‑1 (MQ=255)                                                                cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttg                                                                                                                                >  2:1571090/1‑78 (MQ=255)                                                                cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                                 <  2:346794/93‑1 (MQ=255)                                                                cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGc                                                                                                                 >  1:346794/1‑93 (MQ=255)                                                                          tgttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCgg                                                         >  1:836488/1‑139 (MQ=255)                                                                           gttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                        <  1:532741/139‑1 (MQ=255)                                                                            ttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                       <  2:413057/139‑1 (MQ=255)                                                                            ttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                       <  1:1240362/139‑1 (MQ=255)                                                                               tCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATaa                                                    >  1:964681/1‑139 (MQ=255)                                                                                 cccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATc                                                  >  1:1611949/1‑139 (MQ=255)                                                                                 cccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATc                                                  >  1:990718/1‑139 (MQ=255)                                                                                   cACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                        >  2:1395691/1‑131 (MQ=255)                                                                                   cACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                        <  1:1395691/131‑1 (MQ=255)                                                                                       ccTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATc                                            >  2:1404711/1‑139 (MQ=255)                                                                                         tCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAg                                          >  1:1520681/1‑139 (MQ=255)                                                                                          ccccGCA‑CCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGc                                        >  2:797327/1‑139 (MQ=255)                                                                                           cccGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                        >  2:1519470/1‑123 (MQ=255)                                                                                           cccGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                        <  1:1519470/123‑1 (MQ=255)                                                                                           cccGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGc                                        <  1:1266703/139‑1 (MQ=255)                                                                                             cGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAg                                      >  2:1469641/1‑139 (MQ=255)                                                                                                  ccccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAAt                                                   >  2:330593/1‑121 (MQ=255)                                                                                                  ccccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAAt                                                   <  1:330593/121‑1 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttgt                                                                                                                               <  1:814128/43‑1 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTttgt                                                                                                                               >  2:814128/1‑43 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                           >  1:132022/1‑47 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                           <  2:132022/47‑1 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGcc                                                                                                      >  2:852977/1‑68 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGcc                                                                                                      <  1:852977/68‑1 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCcgcg                                                                                     >  1:577166/1‑85 (MQ=255)                                                                                                    ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCcgcg                                                                                     <  2:577166/85‑1 (MQ=255)                                                                                                     cTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                           <  2:1275057/94‑1 (MQ=255)                                                                                                     cTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                           >  1:1275057/1‑94 (MQ=255)                                                                                                        cTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCt                           >  1:546307/1‑139 (MQ=255)                                                                                                            cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaaa                                                                                                                       <  1:1158779/43‑1 (MQ=255)                                                                                                            cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaaa                                                                                                                       >  2:1158779/1‑43 (MQ=255)                                                                                                            cccAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCtt                       >  2:1520735/1‑139 (MQ=255)                                                                                                                gTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAAc                   <  2:1611949/139‑1 (MQ=255)                                                                                                                  aCGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACCCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGc                 <  2:492478/139‑1 (MQ=255)                                                                                                                    ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca               <  2:1520681/139‑1 (MQ=255)                                                                                                                    ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca               <  1:843481/139‑1 (MQ=255)                                                                                                                     gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAata                                                             >  1:688057/1‑92 (MQ=255)                                                                                                                     gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAata                                                             <  2:688057/92‑1 (MQ=255)                                                                                                                      ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCTGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCcaca             >  2:1539753/1‑139 (MQ=255)                                                                                                                      ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                        <  2:1609243/96‑1 (MQ=255)                                                                                                                      ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                        >  1:1609243/1‑96 (MQ=255)                                                                                                                       cATTCACCACTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCa                                                                                                     >  2:1082402/1‑50 (MQ=38)                                                                                                                       cATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCa                                                                                                     <  1:1082402/50‑1 (MQ=255)                                                                                                                        aTTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAat           >  1:1014860/1‑139 (MQ=255)                                                                                                                        aTTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAat           <  1:1400058/139‑1 (MQ=255)                                                                                                                         ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                       <  2:698616/46‑1 (MQ=255)                                                                                                                         ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                       >  1:698616/1‑46 (MQ=255)                                                                                                                          tCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAatat         <  2:990718/139‑1 (MQ=255)                                                                                                                                aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTggg                                                                  >  1:1215015/1‑76 (MQ=255)                                                                                                                                aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTggg                                                                  <  2:1215015/76‑1 (MQ=255)                                                                                                                                aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCt   >  1:365623/1‑139 (MQ=255)                                                                                                                                 tCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                       >  1:186162/1‑38 (MQ=37)                                                                                                                                 tCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                       <  2:186162/38‑1 (MQ=37)                                                                                                                                 tCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTg  <  2:964681/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTG  >  NC_000913/3695865‑3696131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑