Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,761,850	C→T	A36T (GCG→ACG)	yibF ←	glutathione S‑transferase homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,761,850	0	C	T	100.0%	228.9 / NA	70	A36T (GCG→ACG)	yibF	glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/35);  total (35/35)

TCGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGCGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAATAT  >  NC_000913/3761721‑3761983                                                                                                                                  |                                                                                                                                      tCGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                              <  2:670781/139‑1 (MQ=255)                  attAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAAtt                                                                                                             >  1:503725/1‑139 (MQ=255)                     aaTTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                          <  1:317451/139‑1 (MQ=255)                      aTTCAATATATTCAGCAATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGaa                                                                                                         >  2:1612448/1‑139 (MQ=255)                       ttCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                             <  2:302437/118‑1 (MQ=255)                       ttCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                             >  1:302437/1‑118 (MQ=255)                        tCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCC‑CTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGt                                                                                                      >  1:1242359/1‑139 (MQ=255)                              tatTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                 <  2:1489535/139‑1 (MQ=255)                                 tCAGCAATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              <  1:1612448/139‑1 (MQ=255)                                   aGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              >  2:273173/1‑137 (MQ=255)                                   aGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              <  1:273173/137‑1 (MQ=255)                                    gCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCtttt                                                                                           >  1:1282385/1‑139 (MQ=255)                                     cGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                              >  1:481530/1‑103 (MQ=255)                                     cGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                              <  2:481530/103‑1 (MQ=255)                                        tgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATtaata                                                                                                                 >  2:1285749/1‑113 (MQ=255)                                        tgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATtaata                                                                                                                 <  1:1285749/113‑1 (MQ=255)                                          atCGGCGAATCAAACCAGCATTCGTCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              <  2:825051/130‑1 (MQ=255)                                          atCGGCGAATCAAACCAGCATTCGTCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              >  1:825051/1‑130 (MQ=255)                                          atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                 <  2:471324/127‑1 (MQ=255)                                          atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                 >  1:471324/1‑127 (MQ=255)                                          atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              <  1:339960/130‑1 (MQ=255)                                          atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              >  2:339960/1‑130 (MQ=255)                                          atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaaca                                                                                     >  2:105474/1‑139 (MQ=255)                                             ggCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   <  1:1368679/138‑1 (MQ=255)                                             ggCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   >  2:1368679/1‑138 (MQ=255)                                               cGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGa                                                                                >  1:1453291/1‑139 (MQ=255)                                                                taccatctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTAc                                                               >  1:15589/6‑139 (MQ=255)                                                                 gCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCgtgt                                                                                                                                     >  2:1602196/1‑68 (MQ=255)                                                                      ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   <  1:1390023/113‑1 (MQ=255)                                                                      ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   >  2:1390023/1‑113 (MQ=255)                                                                      ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaaca                                                                                     <  1:92283/111‑1 (MQ=255)                                                                      ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaaca                                                                                     >  2:92283/1‑111 (MQ=255)                                                                                   cACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                             <  1:1305634/58‑1 (MQ=255)                                                                                   cACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                             >  2:1305634/1‑58 (MQ=255)                                                                                   cACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTa                                            >  2:706439/1‑139 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGcc                                                                                                <  2:97133/84‑1 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGcc                                                                                                >  1:97133/1‑84 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                 >  1:188586/1‑83 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                                 <  2:188586/83‑1 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATa                                                                             <  1:328071/103‑1 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATa                                                                             >  2:328071/1‑103 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACgg                                                         >  2:1403146/1‑123 (MQ=255)                                                                                      cGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACgg                                                         <  1:1403146/123‑1 (MQ=255)                                                                                        gCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTta                                                                                                                                   <  2:641440/47‑1 (MQ=255)                                                                                        gCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTta                                                                                                                                   >  1:641440/1‑47 (MQ=255)                                                                                        gCACTTTTCCTAACGGGTAAAATTGCGCCACGCCGTTGTCCGTGTta                                                                                                                                   >  2:518543/1‑47 (MQ=38)                                                                                        gCACTTTTCCTAACGGGTAAAATTGCGCCACGCCGTTGTCCGTGTta                                                                                                                                   <  1:518543/47‑1 (MQ=38)                                                                                             tttCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGtt                                  >  2:633006/1‑139 (MQ=255)                                                                                               tCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTc                                <  2:1282385/139‑1 (MQ=255)                                                                                                        gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              <  1:881496/68‑1 (MQ=255)                                                                                                        gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              >  2:881496/1‑68 (MQ=255)                                                                                                        gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGt                       >  1:411404/1‑139 (MQ=255)                                                                                                         ttAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              <  1:1580022/67‑1 (MQ=255)                                                                                                         ttAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                              >  2:1580022/1‑67 (MQ=255)                                                                                                         ttAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   >  2:92352/1‑78 (MQ=255)                                                                                                         ttAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   <  1:92352/78‑1 (MQ=255)                                                                                                         ttAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTc                      >  2:531629/1‑139 (MQ=255)                                                                                                                  gcCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   >  1:954234/1‑69 (MQ=255)                                                                                                                  gcCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                   <  2:954234/69‑1 (MQ=255)                                                                                                                  gcCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACaaa                                                            >  1:186586/1‑92 (MQ=255)                                                                                                                  gcCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACaaa                                                            <  2:186586/92‑1 (MQ=255)                                                                                                                   ccACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGa            <  1:633006/139‑1 (MQ=255)                                                                                                                      cGCCGTCGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAg                                                                            <  1:1347301/72‑1 (MQ=255)                                                                                                                      cGCCGTCGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAg                                                                            >  2:1347301/1‑72 (MQ=255)                                                                                                                           ttGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAat    <  2:411404/139‑1 (MQ=255)                                                                                                                           ttGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAat    <  2:1242359/139‑1 (MQ=255)                                                                                                                            tGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGtt                                                                      >  2:624639/1‑72 (MQ=255)                                                                                                                            tGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGtt                                                                      <  1:624639/72‑1 (MQ=255)                                                                                                                            tGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAata   <  2:1453291/139‑1 (MQ=255)                                                                                                                             gTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAatat  <  2:503725/139‑1 (MQ=255)                                                                                                                             gTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAatat  <  2:15589/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      TCGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGCGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAATAT  >  NC_000913/3761721‑3761983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑