Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	345,649	G→A	*82* (TAG→TAA)	yahM →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,649	0	G	A	100.0%	144.3 / NA	45	*82* (TAG→TAA)	yahM	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

AAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGGCA  >  NC_000913/345513‑345781                                                                                                                                         |                                                                                                                                     aaaaaGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTaaag                                                                                                                                    <  2:1072338/139‑1 (MQ=255)                 ggAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTc                                                                                                                    <  1:335230/139‑1 (MQ=255)                     agTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTaa                                                                                                                               >  2:1463740/1‑124 (MQ=255)                     agTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTaa                                                                                                                               <  1:1463740/124‑1 (MQ=255)                     agTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                                <  2:174658/139‑1 (MQ=255)                     agTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATAGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                                >  2:859403/1‑139 (MQ=255)                      gTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                               >  1:156777/1‑139 (MQ=255)                      gTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                               >  2:1610809/1‑139 (MQ=255)                          gCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCg                                                                                                           >  1:591162/1‑139 (MQ=255)                                aTGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                                     <  2:591162/139‑1 (MQ=255)                                        cTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTaa                                                                                             >  2:232884/1‑139 (MQ=255)                                          aaaGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAAt                                                                                           >  1:1259416/1‑139 (MQ=255)                                            aGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCa                                                                                         <  2:1259416/139‑1 (MQ=255)                                                 cAATTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                                    <  1:1467595/139‑1 (MQ=255)                                                        aCTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                  >  1:914199/1‑134 (MQ=255)                                                        aCTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                  <  2:914199/134‑1 (MQ=255)                                                         cTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGaa                                                                            <  2:1214265/139‑1 (MQ=255)                                                            aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                  >  2:865440/1‑130 (MQ=255)                                                            aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                  <  1:865440/130‑1 (MQ=255)                                                              cGGAAATAAGGACCACGCATTAC‑GGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGaa                                                                            <  2:931864/133‑1 (MQ=255)                                                              cGGAAATAAGGACCACGCATTAC‑GGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGaa                                                                            >  1:931864/1‑133 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATaca                                                                        >  2:464979/1‑125 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATaca                                                                        <  1:464979/125‑1 (MQ=255)                                                                             cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCTTAGCGCGAATACACCAATAATTACACCaa                                                        >  1:1584251/1‑139 (MQ=255)                                                                             cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCaa                                                        <  1:945745/139‑1 (MQ=255)                                                                             cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCaa                                                        >  1:244053/1‑139 (MQ=255)                                                                                     gggggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCt                                                                                                                     <  1:1504419/70‑1 (MQ=255)                                                                                     gggggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCt                                                                                                                     >  2:1504419/1‑70 (MQ=255)                                                                                       gggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTTATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGaa                                                                            <  2:1004901/109‑1 (MQ=255)                                                                                       gggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGaa                                                                            >  1:1004901/1‑109 (MQ=255)                                                                                          cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGt                                                                                                                                 <  2:1446233/53‑1 (MQ=255)                                                                                          cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGt                                                                                                                                 >  1:1446233/1‑53 (MQ=255)                                                                                            atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCaa                                         >  2:1320886/1‑139 (MQ=255)                                                                                              catcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCa                                                                     <  1:494278/109‑1 (MQ=255)                                                                                              catcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCa                                                                     >  2:494278/1‑109 (MQ=255)                                                                                              catcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAAcg                                       >  2:1503588/1‑139 (MQ=255)                                                                                                 cGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACAGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGt                                    >  2:388743/1‑139 (MQ=255)                                                                                                  gCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCa                                                                                                        <  2:1105086/70‑1 (MQ=255)                                                                                                  gCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCa                                                                                                        >  1:1105086/1‑70 (MQ=255)                                                                                                       tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                              <  2:156777/139‑1 (MQ=255)                                                                                                              gATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCAt                       >  2:1012028/1‑139 (MQ=255)                                                                                                               aTTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCtt                                                                                                  <  2:1534842/63‑1 (MQ=255)                                                                                                               aTTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCtt                                                                                                  >  1:1534842/1‑63 (MQ=255)                                                                                                                     cAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTTGCGCGa                                                                             >  1:703445/1‑78 (MQ=255)                                                                                                                     cAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTTGCGCGa                                                                             <  2:703445/78‑1 (MQ=255)                                                                                                                                   tACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGGCa  <  1:232884/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     AAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGGCA  >  NC_000913/345513‑345781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑