Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,895,071 G→A M140I (ATG→ATA chrR → chromate reductase, Class I, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,895,0710GA100.0% 140.2 / NA 44M140I (ATG→ATAchrRchromate reductase, Class I, flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

TCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATGGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGA  >  NC_000913/3894946‑3895206
                                                                                                                             |                                                                                                                                       
tCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCATTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGCGATGAACACGc                                                                                                                            <  1:932962/139‑1 (MQ=255)
            gATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATgg                                                                                                                >  1:686989/1‑139 (MQ=255)
                aaCCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACATGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGggcgg                                                                                                            >  1:1025648/1‑139 (MQ=255)
                 aCCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGggcggc                                                                                                           >  1:590560/1‑139 (MQ=255)
                       ggCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGAtt                                                                                                     <  2:686989/139‑1 (MQ=255)
                        gCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTc                                                                                                    >  2:1599394/1‑139 (MQ=255)
                             tAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAAc                                                                                               >  1:549015/1‑139 (MQ=255)
                                         gATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCg                                                                                   <  2:549015/139‑1 (MQ=255)
                                          aTTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGc                                                                                  >  1:784289/1‑139 (MQ=255)
                                           ttCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCa                                                                                 <  2:590560/139‑1 (MQ=255)
                                               gACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAAcc                                                                             >  2:1595575/1‑139 (MQ=255)
                                                aCCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCg                                                                            <  1:1595575/139‑1 (MQ=255)
                                                     cTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGaa                                                                       >  1:518097/1‑139 (MQ=255)
                                                      tCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAg                                                                      <  1:1353133/139‑1 (MQ=255)
                                                          tGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGtgat                                                                  >  1:1514614/1‑139 (MQ=255)
                                                           gggCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGtgatt                                                                 <  1:141464/139‑1 (MQ=255)
                                                              cGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGtgattgat                                                              <  1:1412050/139‑1 (MQ=255)
                                                               gTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCg                                                                            <  1:68721/124‑1 (MQ=255)
                                                               gTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCg                                                                            >  2:68721/1‑124 (MQ=255)
                                                                tGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGgcggcg                                                                                                          <  1:121331/93‑1 (MQ=255)
                                                                tGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGgcggcg                                                                                                          >  2:121331/1‑93 (MQ=255)
                                                                  aTTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAgg                                                          <  1:79675/139‑1 (MQ=255)
                                                                            cgcgcTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGtg                                                                                                                                      >  2:1147618/1‑53 (MQ=255)
                                                                            cgcgcTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGtg                                                                                                                                      <  1:1147618/53‑1 (MQ=255)
                                                                            cgcgcTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGa                                                >  2:520830/1‑139 (MQ=255)
                                                                                             cTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGaa                                                                                                >  1:201974/1‑74 (MQ=255)
                                                                                             cTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGaa                                                                                                <  2:201974/74‑1 (MQ=255)
                                                                                             cTGCGCCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAAt                               >  2:961751/1‑139 (MQ=255)
                                                                                               gcgtcAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTg                             >  2:276356/5‑139 (MQ=255)
                                                                                               gcgcCAGATTTTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCg                                                                                   >  2:1151242/1‑85 (MQ=255)
                                                                                               gcgcCAGATTTTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCg                                                                                   <  1:1151242/85‑1 (MQ=255)
                                                                                               gcgcCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGaa                                                                                                <  2:921920/72‑1 (MQ=255)
                                                                                               gcgcCAGATTCTGGTTTACCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGaa                                                                                                >  1:921920/1‑72 (MQ=255)
                                                                                                cgcCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGaa                                                                                                <  2:97669/71‑1 (MQ=255)
                                                                                                cgcCAGATTCTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGaa                                                                                                >  1:97669/1‑71 (MQ=255)
                                                                                                         cTGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTg                   <  1:520830/139‑1 (MQ=255)
                                                                                                          tGGTTTTCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTgg                  <  2:784289/139‑1 (MQ=255)
                                                                                                                tCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGgag                                                                         >  1:943921/1‑78 (MQ=255)
                                                                                                                tCCTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGgag                                                                         <  2:943921/78‑1 (MQ=255)
                                                                                                                 ccTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGtg                                                                    >  2:89614/1‑82 (MQ=255)
                                                                                                                 ccTCGATGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGtg                                                                    <  1:89614/82‑1 (MQ=255)
                                                                                                                      aTGCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCa      <  2:919459/139‑1 (MQ=255)
                                                                                                                        gCAATAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGc    <  2:518097/139‑1 (MQ=255)
                                                                                                                          aaTAGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGa  <  2:1514614/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                       
TCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTCAATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATGGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGA  >  NC_000913/3894946‑3895206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: