| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,908,514 | A→G | intergenic (‑148/+35) | pstB ← / ← pstA | phosphate ABC transporter ATPase/phosphate ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,908,514 | 0 | A | G | 97.6% | 145.7 / NA | 42 | intergenic (‑148/+35) | pstB/pstA | phosphate ABC transporter ATPase/phosphate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (19/22); minor base T (0/1); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCC > NC_000913/3908396‑3908633 | aCTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGaaaa < 2:375194/139‑1 (MQ=255) cTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgtgt > 2:1236338/1‑139 (MQ=255) aTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACTGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAACCGTGTCAg < 2:1078671/139‑1 (MQ=255) aTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAGACCGTGTCAg < 2:766016/139‑1 (MQ=255) gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAgg > 2:486732/1‑119 (MQ=255) gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAgg < 1:486732/119‑1 (MQ=255) gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAata > 2:1399583/1‑139 (MQ=255) ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc < 1:1020307/139‑1 (MQ=255) ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc < 1:1236338/139‑1 (MQ=255) aTCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGtt < 2:474803/94‑1 (MQ=255) aTCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGtt > 1:474803/1‑94 (MQ=255) aTCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc > 1:878782/1‑139 (MQ=255) cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGt < 1:38722/89‑1 (MQ=255) cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGt > 2:38722/1‑89 (MQ=255) cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaaa > 1:364075/1‑139 (MQ=255) cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaaa > 2:581004/1‑139 (MQ=255) cgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcg < 2:364075/139‑1 (MQ=255) aaaGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGaa < 2:878782/139‑1 (MQ=255) ccGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcag < 1:1399583/139‑1 (MQ=255) gagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca < 1:581004/139‑1 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAggg < 2:1555077/53‑1 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAggg > 1:1555077/1‑53 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgc < 1:1043390/110‑1 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgc > 2:1043390/1‑110 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATg < 1:696474/121‑1 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATg > 2:696474/1‑121 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcac > 1:322997/1‑139 (MQ=255) agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcac > 2:679491/1‑139 (MQ=255) cAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc > 2:579799/1‑80 (MQ=255) cAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc < 1:579799/80‑1 (MQ=255) aaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGaaa < 2:303759/58‑1 (MQ=255) aaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGaaa > 1:303759/1‑58 (MQ=255) ggCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATg < 1:679491/139‑1 (MQ=255) cggtcCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAccc < 2:874434/139‑1 (MQ=255) cTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAAc < 1:543421/39‑1 (MQ=11) cTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAAc > 2:543421/1‑39 (MQ=255) cTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCa < 2:282535/46‑1 (MQ=255) cTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCa > 1:282535/1‑46 (MQ=255) cTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc < 1:201793/137‑1 (MQ=255) cTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc > 2:201793/1‑137 (MQ=255) ccTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagca < 2:81596/96‑1 (MQ=255) ccTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagca > 1:81596/1‑96 (MQ=255) | ACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCC > NC_000913/3908396‑3908633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |