Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,386	C→T	R78C (CGT→TGT)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,386	0	C	T	100.0%	168.1 / NA	52	R78C (CGT→TGT)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

CTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGAC  >  NC_000913/3927263‑3927508                                                                                                                            |                                                                                                                           cTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGc                                                                                                             <  2:1069797/139‑1 (MQ=255)         tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCt                                                                                                                   >  1:1281767/1‑125 (MQ=255)         tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCt                                                                                                                   <  2:1281767/125‑1 (MQ=255)         tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGAc                                                                                                     >  2:772277/1‑139 (MQ=255)         tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGAc                                                                                                     >  2:395894/1‑139 (MQ=255)                       cGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGa                                                                                       <  2:1068706/139‑1 (MQ=255)                           ggATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAggg                                                                                   <  1:614000/139‑1 (MQ=255)                               aaCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGt                                                                               <  1:253444/139‑1 (MQ=255)                                aCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTa                                                                              <  2:1469861/139‑1 (MQ=255)                                    gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAacac                                                                                                        >  2:845027/1‑109 (MQ=255)                                    gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAacac                                                                                                        <  1:845027/109‑1 (MQ=255)                                    gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTac                                                                             >  1:392619/1‑136 (MQ=255)                                    gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTac                                                                             <  2:392619/136‑1 (MQ=255)                                     tCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                         <  1:395894/139‑1 (MQ=255)                                      cGGGCTGTGCAACCGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGCTGCCCTGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGTGCGAAGGGCTGTACACGca                                                                        >  1:1282154/1‑139 (MQ=255)                                      cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGca                                                                        >  2:179867/1‑139 (MQ=255)                                        ggCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGcaca                                                                      <  1:1009108/139‑1 (MQ=255)                                           tgtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                              >  1:530362/1‑112 (MQ=255)                                           tgtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                              <  2:530362/112‑1 (MQ=255)                                            gtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACg                                                                          >  2:642885/1‑131 (MQ=255)                                            gtgAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACg                                                                          <  1:642885/131‑1 (MQ=255)                                                       gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                              <  1:1366929/100‑1 (MQ=255)                                                       gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAgcg                                                                                              >  2:1366929/1‑100 (MQ=255)                                                       gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGcc                                                       >  2:635519/1‑139 (MQ=255)                                                       gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGcc                                                       >  2:762958/1‑139 (MQ=255)                                                               aaaaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGaa                                               <  1:635519/139‑1 (MQ=255)                                                               aaaaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGaa                                               <  1:762958/139‑1 (MQ=255)                                                                 aaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGa                                             >  2:701531/1‑139 (MQ=255)                                                                  aGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGAc                                            <  2:833106/139‑1 (MQ=255)                                                                         gCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCttt                                     <  1:179867/139‑1 (MQ=255)                                                                                    tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCaa                                                                                                           <  1:540334/58‑1 (MQ=255)                                                                                    tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCaa                                                                                                           >  2:540334/1‑58 (MQ=255)                                                                                    tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaaa                                                                 >  1:1171092/1‑100 (MQ=255)                                                                                    tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGaaa                                                                 <  2:1171092/100‑1 (MQ=255)                                                                                    tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGcc                                                       >  2:1343728/1‑110 (MQ=255)                                                                                    tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGcc                                                       <  1:1343728/110‑1 (MQ=255)                                                                                    tGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                          >  1:522785/1‑139 (MQ=255)                                                                                         aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCa                                                                                                                        >  2:1523768/1‑40 (MQ=255)                                                                                         aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCa                                                                                                                        <  1:1523768/40‑1 (MQ=255)                                                                                         aGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                     >  1:336109/1‑139 (MQ=255)                                                                                               aaGTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGt               >  2:474227/1‑139 (MQ=255)                                                                                                 gTGGTTCATTCACTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTg             <  1:772277/139‑1 (MQ=255)                                                                                                       cattcaCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt       <  2:522785/139‑1 (MQ=255)                                                                                                            aCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGAc                                                                                                     >  2:1070375/1‑40 (MQ=255)                                                                                                            aCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGAc                                                                                                     <  1:1070375/40‑1 (MQ=255)                                                                                                            aCTGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGAc  <  1:474227/139‑1 (MQ=255)                                                                                                              tGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGa                                             <  2:1447581/94‑1 (MQ=255)                                                                                                              tGGCGAAGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGa                                             >  1:1447581/1‑94 (MQ=255)                                                                                                                   aaGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                     <  2:156586/113‑1 (MQ=255)                                                                                                                   aaGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                     >  1:156586/1‑113 (MQ=255)                                                                                                                    aGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt       <  2:1604176/126‑1 (MQ=255)                                                                                                                    aGTGGAAATGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGt       >  1:1604176/1‑126 (MQ=255)                                                                                                                            |                                                                                                                           CTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGAC  >  NC_000913/3927263‑3927508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑