Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,939,219 G→A *331* (TAG→TAA
S465F (TCT→TTT) 
rbsR →
hsrA ←
transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,939,2190GA100.0% 343.5 / NA 104*331* (TAG→TAA
S465F (TCT→TTT) 
rbsR
hsrA
transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/55);  total (49/55)

Only 100 of 104 total aligned reads displayed.

ACCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTG  >  NC_000913/3939085‑3939347
                                                                                                                                      |                                                                                                                                
aCCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAAttt                                                                                                                              <  2:202417/139‑1 (MQ=255)
   aCTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                           <  2:814334/139‑1 (MQ=255)
     tacccaccaACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                         <  1:1106909/136‑1 (MQ=255)
       tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGt                                                                                                                       >  2:1244298/1‑139 (MQ=255)
       tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGt                                                                                                                       >  2:1482290/1‑139 (MQ=255)
       tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGt                                                                                                                       >  2:803331/1‑139 (MQ=255)
          accaACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          >  1:706628/1‑133 (MQ=255)
          accaACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          <  2:706628/133‑1 (MQ=255)
           ccaACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCttt                                                                                                                   <  1:187503/139‑1 (MQ=255)
            caACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCtttt                                                                                                                  >  1:652457/1‑139 (MQ=255)
               ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          <  1:213928/128‑1 (MQ=255)
               ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          >  2:213928/1‑128 (MQ=255)
                 gAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCa                                                                                                             <  1:557360/139‑1 (MQ=255)
                          aCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTAc                                                                                                    >  1:522536/1‑139 (MQ=255)
                             gggggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                 <  2:465280/139‑1 (MQ=255)
                                ggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                              <  1:174308/139‑1 (MQ=255)
                                    cTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                              >  2:80385/1‑119 (MQ=255)
                                    cTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                              <  1:80385/119‑1 (MQ=255)
                                        cGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                              <  2:1036951/115‑1 (MQ=255)
                                        cGACTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGAGGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                              >  1:1036951/1‑115 (MQ=255)
                                                     catccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                              >  2:24368/1‑118 (MQ=255)
                                                     catccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                              <  1:24368/118‑1 (MQ=255)
                                                     catccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACa                                                                         >  2:139288/1‑139 (MQ=255)
                                                      atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     >  1:109308/1‑126 (MQ=255)
                                                      atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     <  2:1239754/126‑1 (MQ=255)
                                                      atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     <  2:109308/126‑1 (MQ=255)
                                                      atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     >  1:1239754/1‑126 (MQ=255)
                                                      atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                        >  2:871328/1‑139 (MQ=255)
                                                      atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                        >  1:1527530/1‑139 (MQ=255)
                                                        ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     >  2:1044886/1‑124 (MQ=255)
                                                        ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     <  1:1044886/124‑1 (MQ=255)
                                                        ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                      <  1:803331/139‑1 (MQ=255)
                                                              gATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGctgctg                                                                >  1:1410589/1‑139 (MQ=255)
                                                              gATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGctgctg                                                                >  1:378629/1‑139 (MQ=255)
                                                                tAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGaa                                                              <  2:303015/139‑1 (MQ=255)
                                                                  aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          <  2:629248/77‑1 (MQ=255)
                                                                  aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          >  1:629248/1‑77 (MQ=255)
                                                                  aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                 <  1:225792/102‑1 (MQ=255)
                                                                  aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                 >  2:225792/1‑102 (MQ=255)
                                                                  aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                            <  2:652457/139‑1 (MQ=255)
                                                                     cAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     <  1:972770/111‑1 (MQ=255)
                                                                       gCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                       <  1:1244298/139‑1 (MQ=255)
                                                                        ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                               <  2:1035069/82‑1 (MQ=255)
                                                                        ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                               >  1:1035069/1‑82 (MQ=255)
                                                                           aCCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          >  2:35621/1‑68 (MQ=255)
                                                                           aCCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          <  1:35621/68‑1 (MQ=255)
                                                                           aCCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     <  2:372205/105‑1 (MQ=255)
                                                                           aCCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     >  1:372205/1‑105 (MQ=255)
                                                                             ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaataa                                                 >  1:739166/1‑139 (MQ=255)
                                                                             ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaataa                                                 >  2:279044/1‑139 (MQ=255)
                                                                              cTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTaataat                                                <  2:522536/139‑1 (MQ=255)
                                                                                tCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                            <  1:127122/125‑1 (MQ=255)
                                                                                tCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                            >  2:127122/1‑125 (MQ=255)
                                                                                 cggCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                         <  1:1301006/61‑1 (MQ=255)
                                                                                 cAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                         >  2:1301006/1‑63 (MQ=255)
                                                                                  aGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGccc                                            <  2:378629/139‑1 (MQ=255)
                                                                                     aacaacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTGCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAtt                                         <  1:1482290/139‑1 (MQ=255)
                                                                                       caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCtt                                                                                                                    <  2:397935/62‑1 (MQ=255)
                                                                                       caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCtt                                                                                                                    >  1:397935/1‑62 (MQ=255)
                                                                                       caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                            <  2:633241/118‑1 (MQ=255)
                                                                                       caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                            >  1:633241/1‑118 (MQ=255)
                                                                                       caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATtgt                                       <  1:139288/139‑1 (MQ=255)
                                                                                         acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                           >  1:207191/1‑53 (MQ=255)
                                                                                         acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                           <  2:207191/53‑1 (MQ=255)
                                                                                         acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     >  1:127587/1‑91 (MQ=255)
                                                                                         acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                     <  2:127587/91‑1 (MQ=255)
                                                                                         acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                >  1:1001762/1‑96 (MQ=255)
                                                                                         acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                     <  1:279044/139‑1 (MQ=255)
                                                                                          cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                         <  2:1220561/54‑1 (MQ=255)
                                                                                          cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                         >  1:1220561/1‑54 (MQ=255)
                                                                                          cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGAt                                    <  1:1348578/139‑1 (MQ=255)
                                                                                          cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGAt                                    <  2:1527530/139‑1 (MQ=255)
                                                                                            aTTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                               >  1:234230/1‑62 (MQ=255)
                                                                                               aCAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                               <  2:739166/139‑1 (MQ=255)
                                                                                                      aCTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCAt                                                                              >  2:887084/1‑85 (MQ=255)
                                                                                                      aCTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCAt                                                                              <  1:887084/85‑1 (MQ=255)
                                                                                                      aCTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                        <  2:1192219/91‑1 (MQ=255)
                                                                                                      aCTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                        >  1:1192219/1‑91 (MQ=255)
                                                                                                      aCTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTg                        >  2:57022/1‑139 (MQ=255)
                                                                                                       cTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctgc                                                                  >  2:5031/1‑96 (MQ=255)
                                                                                                       cTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctgc                                                                  <  1:5031/96‑1 (MQ=255)
                                                                                                           gATTCTGATGGAACGCGGTTCGGCTTAAATTTATGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                >  1:1415473/1‑78 (MQ=255)
                                                                                                           gATTCTGATGGAACGCGGTTCGGCTTAAATTTATGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                <  2:1415473/78‑1 (MQ=255)
                                                                                                           gATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          >  1:1136847/1‑36 (MQ=255)
                                                                                                           gATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                          <  2:1136847/36‑1 (MQ=255)
                                                                                                            aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTg                  >  2:328038/1‑139 (MQ=255)
                                                                                                            aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTg                  >  2:1330992/1‑139 (MQ=255)
                                                                                                            aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTg                  >  2:708601/1‑139 (MQ=255)
                                                                                                             ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGaa                                                              <  1:828107/94‑1 (MQ=255)
                                                                                                             ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGaa                                                              >  2:828107/1‑94 (MQ=255)
                                                                                                             ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                     >  2:260331/1‑119 (MQ=255)
                                                                                                             ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                     <  1:260331/119‑1 (MQ=255)
                                                                                                             ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGt                 >  2:1169200/1‑139 (MQ=255)
                                                                                                              tCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                 <  2:1166851/58‑1 (MQ=255)
                                                                                                              tCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                 >  1:1166851/1‑58 (MQ=255)
                                                                                                                   tGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACa           <  1:708601/139‑1 (MQ=255)
                                                                                                                            gTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg  <  1:1169200/139‑1 (MQ=255)
                                                                                                                            gTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg  <  2:1410589/139‑1 (MQ=255)
                                                                                                                                ggCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                     <  2:1317969/100‑1 (MQ=255)
                                                                                                                                ggCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                     >  1:1317969/1‑100 (MQ=255)
                                                                                                                                      |                                                                                                                                
ACCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTG  >  NC_000913/3939085‑3939347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: