Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,693	G→A	*610* (TAG→TAA)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,693	0	G	A	95.7%	134.0 / ‑1.6	46	*610* (TAG→TAA)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (20/24);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCG  >  NC_000913/4007558‑4007814                                                                                                                                        |                                                                                                                          gaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATca                                                                                                                        >  1:123269/1‑139 (MQ=255)     gCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagcag                                                                                                                    >  2:218352/1‑139 (MQ=255)      cTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCagcagc                                                                                                                   >  2:1150530/1‑139 (MQ=255)              aTGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACTGCGTTGGGATTCGCAAGCTGGAACGCGTTGGCAAACCGTTTATGGCGCTGTTTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATaaaaaa                                                                                                           >  2:1025923/1‑139 (MQ=255)                   gATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGcc                                                                                                      <  2:1093224/139‑1 (MQ=255)                           ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGTTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATca                                                                                                                        <  1:283279/113‑1 (MQ=255)                           ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                       <  2:928457/114‑1 (MQ=255)                           ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                       >  1:928457/1‑114 (MQ=255)                           ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATca                                                                                                                        >  2:283279/1‑113 (MQ=255)                           ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTg                                                                                                        <  2:1275489/129‑1 (MQ=255)                           ccAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTg                                                                                                        >  1:1275489/1‑129 (MQ=255)                                    tGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                     <  1:1554689/139‑1 (MQ=255)                                    tGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                     <  1:1025923/139‑1 (MQ=255)                                        cAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACg                                                                                 <  1:1268545/139‑1 (MQ=255)                                                     gTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                     <  1:215418/122‑1 (MQ=255)                                                     gTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTa                                                                                     >  2:215418/1‑122 (MQ=255)                                                     gTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcgc                                                                       >  2:1541319/1‑136 (MQ=255)                                                     gTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcgc                                                                       <  1:1541319/136‑1 (MQ=255)                                                      ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGt                                                                   <  2:123269/139‑1 (MQ=255)                                                      ttGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGt                                                                   <  1:1150530/139‑1 (MQ=255)                                                              cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagca                                                                                                                     <  2:403242/81‑1 (MQ=255)                                                              cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcagca                                                                                                                     >  1:403242/1‑81 (MQ=255)                                                              cgcAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAatgta                                                           <  1:1010594/139‑1 (MQ=255)                                                                    cTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                        >  1:890564/1‑120 (MQ=255)                                                                    cTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcg                                                                        <  2:890564/120‑1 (MQ=255)                                                                     tGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGAt                                                    <  1:495160/139‑1 (MQ=255)                                                                                         tttATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcgc                                                                       >  1:526143/1‑100 (MQ=255)                                                                                         tttATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCcgc                                                                       <  2:526143/100‑1 (MQ=255)                                                                                         tttATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCa                                <  2:1537342/139‑1 (MQ=255)                                                                                           tATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATg                              >  2:1128297/1‑139 (MQ=255)                                                                                                 gcTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                       <  1:879761/44‑1 (MQ=255)                                                                                                 gcTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATcag                                                                                                                       >  2:879761/1‑44 (MQ=255)                                                                                                    gATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTg                                                                                                        <  2:1252942/56‑1 (MQ=255)                                                                                                    gATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTg                                                                                                        >  1:1252942/1‑56 (MQ=255)                                                                                                          tGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAat                                                              <  1:830774/92‑1 (MQ=255)                                                                                                          tGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAat                                                              >  2:830774/1‑92 (MQ=255)                                                                                                          tGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                            >  1:797019/1‑62 (MQ=255)                                                                                                          tGCGCATGTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGa                                                                                            <  2:797019/62‑1 (MQ=255)                                                                                                                 gTTGATGGCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGa        >  1:582100/1‑139 (MQ=255)                                                                                                                       ggCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGaa                                                                                              <  1:722237/47‑1 (MQ=255)                                                                                                                       ggCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGaa                                                                                              >  2:722237/1‑47 (MQ=255)                                                                                                                       ggCGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCg  <  2:626203/139‑1 (MQ=255)                                                                                                                         cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGt                            >  1:1600969/1‑111 (MQ=255)                                                                                                                         cGATGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGt                            <  2:1600969/111‑1 (MQ=255)                                                                                                                            tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGtt                                                       >  1:1517753/1‑81 (MQ=255)                                                                                                                            tGACGAAGAGTAATCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGtt                                                       <  2:1517753/81‑1 (MQ=255)                                                                                                                                        |                                                                                                                          GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCG  >  NC_000913/4007558‑4007814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑