Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,204,645 G→A *442* (TAG→TAA
N429N (AAC→AAT
zraR →
purD ←
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,204,6450GA100.0% 145.8 / NA 45*442* (TAG→TAA
N429N (AAC→AAT
zraR
purD
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/22);  total (23/22)

TCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCG  >  NC_000913/4204516‑4204766
                                                                                                                                 |                                                                                                                         
tCAGCCGTTGGTGGAAGTGGAAAAAGGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTcgc                                                                                                                 >  1:1022535/1‑139 (MQ=255)
       ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGa                                                                                                           >  1:1232662/1‑139 (MQ=255)
       ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGa                                                                                                           >  1:912221/1‑139 (MQ=255)
        tggtggAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGAt                                                                                                          <  1:1588565/139‑1 (MQ=255)
         ggtggAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTc                                                                                                                   >  1:1034498/1‑129 (MQ=255)
         ggtggAAGTGGAAAAAGAGGTGATCCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTc                                                                                                                   <  2:1034498/129‑1 (MQ=255)
                    aaaaaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGcc                                                                                              >  1:935417/1‑139 (MQ=255)
                           ggTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCt                                                                                       >  1:184763/1‑139 (MQ=255)
                            gTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCtt                                                                                      >  1:1101859/1‑139 (MQ=255)
                                                gaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                  <  2:1022535/139‑1 (MQ=255)
                                                 aaaaCGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTccc                                                                 <  2:420092/139‑1 (MQ=255)
                                                        gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGat                                                          >  1:862355/1‑139 (MQ=255)
                                                         cggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGata                                                         <  2:1101859/139‑1 (MQ=255)
                                                            caacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                      <  2:197149/139‑1 (MQ=255)
                                                                            gccCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGc                                                                          <  2:387246/103‑1 (MQ=255)
                                                                            gccCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGc                                                                          >  1:387246/1‑103 (MQ=255)
                                                                            gccCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAgg                                      >  1:129938/1‑139 (MQ=255)
                                                                             cccGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgc                                     >  1:851894/1‑139 (MQ=255)
                                                                                          gATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCt                        >  2:1369226/1‑139 (MQ=255)
                                                                                           aTCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCtt                       >  2:115327/1‑139 (MQ=255)
                                                                                             cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTc                                                                                                                   >  1:838468/1‑45 (MQ=255)
                                                                                             cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTc                                                                                                                   <  2:838468/45‑1 (MQ=255)
                                                                                              aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTg                    >  2:716849/1‑139 (MQ=255)
                                                                                               cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGc                                                                                                                     <  1:3669/41‑1 (MQ=255)
                                                                                               cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGc                                                                                                                     >  2:3669/1‑41 (MQ=255)
                                                                                               cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat                                                                                           >  1:484033/1‑67 (MQ=255)
                                                                                               cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat                                                                                           <  2:484033/67‑1 (MQ=255)
                                                                                               cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                   <  2:851894/139‑1 (MQ=255)
                                                                                                 cgcAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                 <  1:801782/139‑1 (MQ=255)
                                                                                                 cgcAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                 <  2:184763/139‑1 (MQ=255)
                                                                                                      aaCGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTa            <  1:1369226/139‑1 (MQ=255)
                                                                                                              tGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAg    <  2:862355/139‑1 (MQ=255)
                                                                                                                gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg  <  2:912221/139‑1 (MQ=255)
                                                                                                                     aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCtt                                                                                      <  2:921098/50‑1 (MQ=255)
                                                                                                                     aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCtt                                                                                      >  1:921098/1‑50 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                   >  2:232199/1‑64 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                   >  2:1289437/1‑64 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                   <  1:1289437/64‑1 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                   <  1:232199/64‑1 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAg                                                               >  2:1466030/1‑68 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAg                                                               <  1:1466030/68‑1 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTg                            >  2:1221650/1‑103 (MQ=255)
                                                                                                                          cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTg                            <  1:1221650/103‑1 (MQ=255)
                                                                                                                           gcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGt                                                              <  2:1375142/68‑1 (MQ=255)
                                                                                                                           gcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGt                                                              >  1:1375142/1‑68 (MQ=255)
                                                                                                                                 |                                                                                                                         
TCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCG  >  NC_000913/4204516‑4204766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: