Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,369,494 C→T A307T (GCA→ACA)  yjeH ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,369,4940CT98.0% 304.2 / ‑2.3 99A307T (GCA→ACA) yjeHputative transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (48/49);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

ATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGCCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATAACGCTCCTACACCGAACAA  >  NC_000913/4369358‑4369629
                                                                                                                                        |                                                                                                                                       
ataaAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCa                                                                                                                                       >  2:420930/1‑139 (MQ=255)
    aGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTa                                                                                                                                   <  1:102951/139‑1 (MQ=255)
     gATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAg                                                                                                                                  >  1:94530/1‑139 (MQ=255)
      aTGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGt                                                                                                                                 >  2:471483/1‑139 (MQ=255)
        gCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTgg                                                                                                                               >  2:1329697/1‑139 (MQ=255)
        gCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTgg                                                                                                                               <  1:530380/139‑1 (MQ=255)
         ccATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              <  1:420930/139‑1 (MQ=255)
                         taaGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              <  2:263882/123‑1 (MQ=255)
                         taaGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              >  1:263882/1‑123 (MQ=255)
                               cGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              >  2:960213/1‑117 (MQ=255)
                               cGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              <  1:960213/117‑1 (MQ=255)
                               cGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCga                                                                                                        >  2:994822/1‑139 (MQ=255)
                               cGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCga                                                                                                        >  2:572033/1‑139 (MQ=255)
                                gTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              <  2:239948/116‑1 (MQ=255)
                                gTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              >  1:239948/1‑116 (MQ=255)
                                gTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgac                                                                                                       >  2:127292/1‑139 (MQ=255)
                                gTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgac                                                                                                       >  2:1070887/1‑139 (MQ=255)
                                gTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgac                                                                                                       >  2:1131108/1‑139 (MQ=255)
                                 tCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              >  1:600479/1‑115 (MQ=255)
                                 tCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              <  2:600479/115‑1 (MQ=255)
                                 tCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgacc                                                                                                      >  2:965095/1‑139 (MQ=255)
                                 tCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgacc                                                                                                      >  1:706036/1‑139 (MQ=255)
                                     gATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgaccagac                                                                                                  <  2:706036/139‑1 (MQ=255)
                                        tGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGaccagaccag                                                                                               <  1:572033/139‑1 (MQ=255)
                                          aTCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggc                                                                                             >  1:314700/1‑139 (MQ=255)
                                               tAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        <  1:127292/139‑1 (MQ=255)
                                               tAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        <  1:1070887/139‑1 (MQ=255)
                                                  aGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAg                                                                                     <  1:185170/139‑1 (MQ=255)
                                                   gCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGc                                                                                    <  1:1343293/139‑1 (MQ=255)
                                                    cATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGa                                                                                                                         <  1:357748/101‑1 (MQ=255)
                                                    cATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGa                                                                                                                         >  2:357748/1‑101 (MQ=255)
                                                    cATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  1:965095/139‑1 (MQ=255)
                                                    cATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  2:94530/139‑1 (MQ=255)
                                                    cATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGCCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgaccagac                                                                                                  <  2:792122/124‑1 (MQ=255)
                                                    cATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGCCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgaccagac                                                                                                  >  1:792122/1‑124 (MQ=255)
                                                     aTGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGctc                                                                                  <  1:471483/139‑1 (MQ=255)
                                                      tGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        >  2:1169564/1‑132 (MQ=255)
                                                      tGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        <  1:1169564/132‑1 (MQ=255)
                                                      tGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGctct                                                                                 >  1:487438/1‑139 (MQ=255)
                                                      tGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGctct                                                                                 <  1:994822/139‑1 (MQ=255)
                                                         aTCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGta                                                                              >  2:722445/1‑139 (MQ=255)
                                                         aTCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGta                                                                              >  2:197111/1‑139 (MQ=255)
                                                                  gTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  2:118826/125‑1 (MQ=255)
                                                                  gTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   >  1:118826/1‑125 (MQ=255)
                                                                   tGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgac                                                                                                       >  2:372442/1‑104 (MQ=255)
                                                                   tGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCgac                                                                                                       <  1:372442/104‑1 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              >  2:27351/1‑79 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGt                                                                                                                              <  1:27351/79‑1 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        <  2:1241380/117‑1 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        >  1:1241380/1‑117 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   >  2:1447915/1‑122 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   >  2:222560/1‑122 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  1:222560/122‑1 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  1:1447915/122‑1 (MQ=255)
                                                                     cTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTga                                                                  >  2:387225/1‑139 (MQ=255)
                                                                           acacaGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTg                                                                                                                 >  2:1495405/1‑86 (MQ=255)
                                                                           acacaGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTg                                                                                                                 <  1:1495405/86‑1 (MQ=255)
                                                                               agcagcCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTa                                                                                                                                   <  2:1338365/64‑1 (MQ=255)
                                                                               agcagcCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTa                                                                                                                                   >  1:1338365/1‑64 (MQ=255)
                                                                               agcagcCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        <  1:776351/107‑1 (MQ=255)
                                                                               agcagcCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAggcgggcg                                                                                        >  2:776351/1‑107 (MQ=255)
                                                                                  agcCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCAAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCa                                                     >  2:177361/1‑139 (MQ=255)
                                                                                   gcCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAg                                                    <  2:314700/139‑1 (MQ=255)
                                                                                    ccGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGc                                                                                    >  2:1213571/1‑106 (MQ=255)
                                                                                    ccGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGc                                                                                    <  1:1213571/106‑1 (MQ=255)
                                                                                    ccGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAgg                                                   >  2:248406/1‑139 (MQ=255)
                                                                                      gAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGcc                                                 <  2:453041/139‑1 (MQ=255)
                                                                                       aGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTGGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCaa                                                         >  1:1240991/1‑130 (MQ=255)
                                                                                       aGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCaa                                                         <  2:1240991/130‑1 (MQ=255)
                                                                                         cACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGa                                              >  2:1192579/1‑139 (MQ=255)
                                                                                              cATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGcc                                         >  1:106635/1‑139 (MQ=255)
                                                                                                     ggCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGcc                                                                                                             <  1:1039154/64‑1 (MQ=255)
                                                                                                     ggCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGcc                                                                                                             >  2:1039154/1‑64 (MQ=255)
                                                                                                     ggCATTATTCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACg                                  <  1:1329697/139‑1 (MQ=255)
                                                                                                         ttattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAg                                                                                     <  1:1468505/84‑1 (MQ=255)
                                                                                                         ttattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAg                                                                                     <  1:1383708/84‑1 (MQ=255)
                                                                                                         ttattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAg                                                                                     >  2:1468505/1‑84 (MQ=255)
                                                                                                         ttattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAg                                                                                     >  2:1383708/1‑84 (MQ=255)
                                                                                                         ttattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  1:1343316/86‑1 (MQ=255)
                                                                                                         ttattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   >  2:1343316/1‑86 (MQ=255)
                                                                                                         ttattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAgg                              <  1:722445/139‑1 (MQ=255)
                                                                                                          tattCGGGATATGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGc                             <  1:248406/139‑1 (MQ=255)
                                                                                                                   tatGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATaa                    <  1:1131108/139‑1 (MQ=255)
                                                                                                                   tatGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATaa                    <  1:1321641/139‑1 (MQ=255)
                                                                                                                   tatGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATaa                    <  2:123642/139‑1 (MQ=255)
                                                                                                                    atGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  1:615546/75‑1 (MQ=255)
                                                                                                                    atGGCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   >  2:615546/1‑75 (MQ=255)
                                                                                                                      ggCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAgg                                                                                              <  1:833770/62‑1 (MQ=255)
                                                                                                                      ggCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAgg                                                                                              >  2:833770/1‑62 (MQ=255)
                                                                                                                      ggCGAGAAGAGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATAACGc                 >  1:479663/1‑139 (MQ=255)
                                                                                                                           gaagaGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   >  2:737390/1‑68 (MQ=255)
                                                                                                                           gaagaGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGct                                                                                   <  1:737390/68‑1 (MQ=255)
                                                                                                                           gaagaGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTgag                                                                 >  2:1010815/1‑86 (MQ=255)
                                                                                                                           gaagaGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTgag                                                                 <  1:1010815/86‑1 (MQ=255)
                                                                                                                           gaagaGAGGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATAACGCTCCTa            <  2:106635/139‑1 (MQ=255)
                                                                                                                                 agGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTgag                                                                 >  1:983721/1‑80 (MQ=255)
                                                                                                                                 agGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTgag                                                                 <  2:983721/80‑1 (MQ=255)
                                                                                                                                 agGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATaa                    >  2:495334/1‑125 (MQ=255)
                                                                                                                                 agGCGTGTCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATaa                    <  1:495334/125‑1 (MQ=255)
                                                                                                                                     gtgtCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATAACGCTCCTACACCGaacaa  <  1:387225/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCATTATTCGGGATATGGCGAGAAGAGAGGCGTGCCAGGTAGTGGTCAGGATTATGTTGCGCCTGCGACCAGACCAGGCGGGCGAAGCTCTGTATATAAATGTTGAGACTGGCAAAGCAGGCCAGATAGCCAATCACGCAGGCAATCCATAACGCTCCTACACCGAACAA  >  NC_000913/4369358‑4369629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: