Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,433,164	C→T	*287* (TAG→TAA)	qorB ←	NAD(P)H:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,433,164	0	C	T	100.0%	115.6 / NA	37	*287* (TAG→TAA)	qorB	NAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

GGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCC  >  NC_000913/4433033‑4433293                                                                                                                                    |                                                                                                                                  ggCGTACATCACGACGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACGTTAATTAATTAGttat                                                                                                                            <  1:1377711/139‑1 (MQ=255) ggCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGttat                                                                                                                            <  1:1321103/139‑1 (MQ=255)   cGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGttatta                                                                                                                          <  1:437331/139‑1 (MQ=255)    gTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTaa                                                                                                                         >  1:1030180/1‑139 (MQ=255)    gTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCAGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTaa                                                                                                                         >  1:979397/1‑139 (MQ=255)    gTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCAGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTaa                                                                                                                         >  1:1287859/1‑139 (MQ=255)              ccgcTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAg                                                                                                               <  2:1030180/139‑1 (MQ=255)                           cTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCtt                                                                                                  <  1:1051526/139‑1 (MQ=255)                              gCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGttatta                                                                                                                          <  1:1146735/112‑1 (MQ=255)                              gCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGttatta                                                                                                                          >  2:1146735/1‑112 (MQ=255)                              gCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCt                                                                                                   <  1:583157/135‑1 (MQ=255)                              gCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCt                                                                                                   >  2:583157/1‑135 (MQ=255)                                aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAattaatta                                                                                                                                 >  1:1000920/1‑103 (MQ=255)                                aTCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGc                                                                                             >  1:1040551/1‑139 (MQ=255)                                       gATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGtt                                                                                      <  2:1040551/139‑1 (MQ=255)                                             tCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGtt                                                                                >  2:47384/1‑139 (MQ=255)                                               agaagaAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTgg                                                                              >  1:176923/1‑139 (MQ=255)                                                  agaaAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                           >  1:1319940/1‑123 (MQ=255)                                                  agaaAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                           >  1:332105/1‑123 (MQ=255)                                                  agaaAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                           <  2:332105/123‑1 (MQ=255)                                                  agaaAGTGCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTa                                                                                           <  2:1319940/123‑1 (MQ=255)                                                                             cTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCt                                                >  2:27077/1‑139 (MQ=255)                                                                                       cccGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACg                                                                                        >  2:252920/1‑89 (MQ=255)                                                                                       cccGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACg                                                                                        <  1:252920/89‑1 (MQ=255)                                                                                          gTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGa                                   <  1:674715/139‑1 (MQ=255)                                                                                          gTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGa                                   <  1:27077/139‑1 (MQ=255)                                                                                             aTTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCg                                >  1:1397768/1‑139 (MQ=255)                                                                                                aTCAGAGAGGATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCt                             <  2:176923/139‑1 (MQ=255)                                                                                                         gATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCtt                                                                                                        >  1:1022328/1‑55 (MQ=255)                                                                                                         gATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCtt                                                                                                        <  2:1022328/55‑1 (MQ=255)                                                                                                         gATGCGGGAGGATGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATgcg                    >  2:807699/1‑139 (MQ=255)                                                                                                                     tGCCACTTTAATTAATTAGTTATTAACATTAAAAAGa                                                                                                              <  1:335345/37‑1 (MQ=255)                                                                                                                     tGCCACTTTAATTAATTAGTTATTAACATTAAAAAGa                                                                                                              >  2:335345/1‑37 (MQ=255)                                                                                                                     tGCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGaa        >  2:1030925/1‑139 (MQ=255)                                                                                                                      gCCACTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAAt       >  1:1469739/1‑139 (MQ=255)                                                                                                                         aCTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCg    <  1:1030925/139‑1 (MQ=255)                                                                                                                          cTTTAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGc   >  2:753931/1‑139 (MQ=255)                                                                                                                           tttAATTAATTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGcc  <  2:1397768/139‑1 (MQ=255)                                                                                                                               attaattaGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACg                                                                                        <  1:344448/49‑1 (MQ=255)                                                                                                                               attaattaGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACg                                                                                        >  2:344448/1‑49 (MQ=255)                                                                                                                                    |                                                                                                                                  GGCGTACAGCACGCCGCTAATCATTACTGGCATCCATTGATCTGTCAGAAGAAAGTTCAGGAAGCAGACATGATTGCTCCCGGCATCCCGTCATTATCAGAGAGGATGCGGGAGGATGCCACTTTAATTAACTAGTTATTAACATTAAAAAGATGGCTTACGCTTTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCC  >  NC_000913/4433033‑4433293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑