Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,456,515	A→G	Q244R (CAG→CGG)	mpl →	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,456,515	0	A	G	100.0%	215.3 / NA	60	Q244R (CAG→CGG)	mpl	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/30);  total (30/30)

TTCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGC  >  NC_000913/4456394‑4456636                                                                                                                          |                                                                                                                          ttCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGa                                                                                                          >  2:1156940/1‑139 (MQ=255)  tCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGaa                                                                                                         >  1:392977/1‑139 (MQ=255)             gTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacca                                                                                              >  1:822296/1‑139 (MQ=255)                            gggCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTg                                                                                                  <  2:1400369/120‑1 (MQ=255)                            gggCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTg                                                                                                  >  1:1400369/1‑120 (MQ=255)                                 gggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCgggg                                                                                                                        >  2:1386329/1‑93 (MQ=255)                                 gggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCgggg                                                                                                                        <  1:1386329/93‑1 (MQ=255)                                  ggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     >  2:76931/1‑111 (MQ=255)                                  ggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     <  1:76931/111‑1 (MQ=255)                                                 ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATg                                                             <  2:865474/136‑1 (MQ=255)                                                 ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATg                                                             >  1:865474/1‑136 (MQ=255)                                                 ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCg                                                          >  1:1243370/1‑139 (MQ=255)                                                 ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCg                                                          >  1:500009/1‑139 (MQ=255)                                                   ggAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGaa                                                        >  1:1518845/1‑139 (MQ=255)                                                           aCATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTggg                                                <  1:286460/139‑1 (MQ=255)                                                               cAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCg                                              <  1:1283081/137‑1 (MQ=255)                                                               cAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCg                                              >  2:1283081/1‑137 (MQ=255)                                                                       aaCAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCTCCGATTCTTCCGAATTGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCTAAGTGAAATg                                    <  1:1570238/139‑1 (MQ=255)                                                                          aGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTc                                 <  2:392977/139‑1 (MQ=255)                                                                           gACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCg                                <  1:647971/139‑1 (MQ=255)                                                                            aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGaa                                       <  2:1550493/131‑1 (MQ=255)                                                                            aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGaa                                       >  1:1550493/1‑131 (MQ=255)                                                                            aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGc                               >  2:119530/1‑139 (MQ=255)                                                                            aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGACGGCGAAAAAGTGGGCGAAGTGAAATGGTCGc                               >  1:1362299/1‑139 (MQ=255)                                                                             ccATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     <  1:537453/68‑1 (MQ=255)                                                                             ccATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     >  2:537453/1‑68 (MQ=255)                                                                               aTGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTgg                            <  2:1362299/139‑1 (MQ=255)                                                                                tGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGt                           >  1:1142237/1‑139 (MQ=255)                                                                                   cGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAgg                        <  1:119530/139‑1 (MQ=255)                                                                                     aTGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGa                                                                                                          <  1:1036197/55‑1 (MQ=255)                                                                                     aTGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGa                                                                                                          >  2:1036197/1‑55 (MQ=255)                                                                                      tGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               >  2:181615/1‑65 (MQ=255)                                                                                      tGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               <  1:181615/65‑1 (MQ=255)                                                                                      tGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGa                     <  2:1142237/139‑1 (MQ=255)                                                                                        ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               >  1:1383320/1‑63 (MQ=255)                                                                                        ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               <  2:1383320/63‑1 (MQ=255)                                                                                        ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACGACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:1156940/139‑1 (MQ=255)                                                                                        ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  2:439391/139‑1 (MQ=255)                                                                                        ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:1230381/139‑1 (MQ=255)                                                                                        ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   >  1:1236646/1‑139 (MQ=255)                                                                                        ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  2:1518845/139‑1 (MQ=255)                                                                                            gctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   >  2:1248776/1‑135 (MQ=255)                                                                                            gctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:1248776/135‑1 (MQ=255)                                                                                             ctgGAGCGAGCAGGGGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:763830/134‑1 (MQ=255)                                                                                             ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTg                                                                    >  1:253479/1‑85 (MQ=255)                                                                                             ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTg                                                                    <  2:253479/85‑1 (MQ=255)                                                                                             ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAgg                        >  2:1533842/1‑129 (MQ=255)                                                                                             ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAgg                        <  1:1533842/129‑1 (MQ=255)                                                                                             ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   >  2:763830/1‑134 (MQ=255)                                                                                                     agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGa                     <  1:622741/124‑1 (MQ=255)                                                                                                     agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGa                     >  2:622741/1‑124 (MQ=255)                                                                                                     agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAatat            <  1:1406603/133‑1 (MQ=255)                                                                                                     agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAatat            >  2:1406603/1‑133 (MQ=255)                                                                                                     agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACaa      >  2:354185/1‑139 (MQ=255)                                                                                                     agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTACGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACaa      >  2:850305/1‑139 (MQ=255)                                                                                                         ggAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGc  <  2:500009/139‑1 (MQ=255)                                                                                                          gAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCg                                >  2:1273977/1‑108 (MQ=255)                                                                                                          gAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCg                                <  1:1273977/108‑1 (MQ=255)                                                                                                                  gggtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTggg                                                <  2:384949/84‑1 (MQ=255)                                                                                                                  gggtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTggg                                                >  1:384949/1‑84 (MQ=255)                                                                                                                    gtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAg                                                                         >  2:1459216/1‑57 (MQ=255)                                                                                                                    gtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAg                                                                         <  1:1459216/57‑1 (MQ=255)                                                                                                                          |                                                                                                                          TTCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGC  >  NC_000913/4456394‑4456636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑