Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,141 C→T *333* (TAG→TAA idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,1410CT100.0% 213.1 / NA 67*333* (TAG→TAAidnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/35);  total (32/35)

GTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACG  >  NC_000913/4490012‑4490270
                                                                                                                                 |                                                                                                                                 
cattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCgtg                                                                                                                          <  2:289026‑M2/24‑1 (MQ=255)
   tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                       >  1:1318401‑M2/113‑139 (MQ=255)
   tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                       <  1:833135‑M2/27‑1 (MQ=255)
   tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                       >  1:662733‑M2/113‑139 (MQ=255)
           gtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa                                                                                                               <  2:501976‑M2/35‑1 (MQ=255)
            taaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATaa                                                                                                              >  2:301021‑M2/104‑139 (MQ=255)
                    gttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATa                                                                                                      >  1:383481‑M2/96‑139 (MQ=255)
                     ttctgtgtccggcgttcgggcaattaaaaggggttcactcttcacataccctccagtttcctgcctggagtataggcaacgtccggcaaagaggGAAGAGAAAATAAGTAAAAGCGTGTTGCCGTGATAAAACTGATAc                                                                                                     <  1:349816‑M2/45‑1 (MQ=255)
                       ctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                   <  2:1034516‑M2/47‑1 (MQ=255)
                        tgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCg                                                                                                  <  1:1338427‑M2/48‑1 (MQ=255)
                          tgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                >  1:1042276‑M2/90‑139 (MQ=255)
                          tgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                >  2:601084‑M2/90‑139 (MQ=255)
                            tccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGt                                                                                              <  2:1042276‑M2/52‑1 (MQ=255)
                               ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           <  2:1423777‑M2/55‑1 (MQ=255)
                               ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           >  2:1005319‑M2/85‑139 (MQ=255)
                                gcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAAc                                                                                          <  1:1005319‑M2/56‑1 (MQ=255)
                                 cgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           >  1:544842‑M2/83‑137 (MQ=255)
                                 cgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           <  2:544842‑M2/55‑1 (MQ=255)
                                 cgtgcgggcaattaacaggggttcactcatgaaatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACa                                                                                         >  1:956392‑M2/83‑139 (MQ=255)
                                  gtgcgggcgattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                        <  2:956392‑M2/58‑1 (MQ=255)
                                  gtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                        <  1:301021‑M2/58‑1 (MQ=255)
                                  gtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                        <  1:601084‑M2/58‑1 (MQ=255)
                                           attaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggt                                                                               >  2:1604479‑M2/73‑139 (MQ=255)
                                                        cactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                      <  2:1437011‑M2/28‑1 (MQ=255)
                                                        cactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                      >  1:1437011‑M2/60‑87 (MQ=255)
                                                          ctcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTAtttt                                                                <  2:1318401‑M2/82‑1 (MQ=255)
                                                           tcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           <  1:1489389‑M2/55‑1 (MQ=255)
                                                           tcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           >  2:1489389‑M2/57‑111 (MQ=255)
                                                             atgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTaa                                                             >  2:758490‑M2/55‑139 (MQ=255)
                                                                   tatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGc                                                       <  2:662733‑M2/91‑1 (MQ=255)
                                                                    atcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCt                                                      >  2:352455‑M2/48‑139 (MQ=255)
                                                                     tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGa                                                                                                 <  2:1535817‑M2/49‑1 (MQ=255)
                                                                     tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGa                                                                                                 >  1:1535817‑M2/47‑95 (MQ=255)
                                                                     tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTc                                                     >  1:1325042‑M2/47‑139 (MQ=255)
                                                                      gtataagagacaggcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCa                                                    >  2:607464‑M2/46‑139 (MQ=255)
                                                                             tttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAAt                                                                                                            <  2:244671‑M2/38‑1 (MQ=255)
                                                                             tttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAAt                                                                                                            >  1:244671‑M2/39‑76 (MQ=255)
                                                                                       ggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGcc                                   <  1:607464‑M2/111‑1 (MQ=255)
                                                                                          gtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                                <  2:1325042‑M2/114‑1 (MQ=255)
                                                                                            ataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCg                              <  1:1604479‑M2/116‑1 (MQ=255)
                                                                                             taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                      <  1:473575‑M2/28‑1 (MQ=255)
                                                                                             taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                      >  2:473575‑M2/23‑50 (MQ=255)
                                                                                              aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGc                                                                                                                             <  1:1476300‑M2/21‑1 (MQ=255)
                                                                                              aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGc                                                                                                                             >  2:1476300‑M2/22‑42 (MQ=255)
                                                                                              aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                                     >  2:617125‑M2/22‑66 (MQ=255)
                                                                                              aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                                     <  1:617125‑M2/45‑1 (MQ=255)
                                                                                                   acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                    >  2:619627‑M2/17‑78 (MQ=255)
                                                                                                   acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                    <  1:619627‑M2/62‑1 (MQ=255)
                                                                                                   acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCGATCCGACCAata                       >  1:281959‑M2/17‑139 (MQ=255)
                                                                                                   acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAata                       >  2:116139‑M2/17‑139 (MQ=255)
                                                                                                    cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                        >  2:1188095‑M2/16‑73 (MQ=255)
                                                                                                    cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                        <  1:1188095‑M2/58‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCt                                                                                                          <  1:856416‑M2/40‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCt                                                                                                          >  2:856416‑M2/15‑54 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                    <  1:1118565‑M2/62‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                    >  2:1118565‑M2/15‑76 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggtt                                                                              <  2:1050880‑M2/68‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                                <  1:837212‑M2/114‑1 (MQ=255)
                                                                                                     gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                                >  2:837212‑M2/15‑128 (MQ=255)
                                                                                                       ccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                       >  1:112532‑M2/13‑39 (MQ=255)
                                                                                                       ccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                       <  2:112532‑M2/27‑1 (MQ=255)
                                                                                                       ccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCaa                   >  1:466139‑M2/13‑139 (MQ=255)
                                                                                                          gcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                      >  1:1202906‑M2/10‑37 (MQ=255)
                                                                                                          gcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                      <  2:1202906‑M2/28‑1 (MQ=255)
                                                                                                                    aaGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           <  1:863678/54‑1 (MQ=255)
                                                                                                                    aaGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                           >  2:863678/1‑54 (MQ=255)
                                                                                                                        gaAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACg  <  2:383481/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                 
GTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACG  >  NC_000913/4490012‑4490270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: