Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,538,785	C→T	*239* (TAG→TAA)	nanC ←	N‑acetylnuraminic acid outer membrane channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,785	0	C	T	100.0%	184.1 / NA	57	*239* (TAG→TAA)	nanC	N‑acetylnuraminic acid outer membrane channel protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/32);  total (25/32)

TCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAG  >  NC_000913/4538650‑4538916                                                                                                                                        |                                                                                                                                    tCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGCGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCtattac                                                                                                                                  >  2:524964/1‑139 (MQ=255)       tCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACCGTTTa                                                                                                                            <  1:1004723/139‑1 (MQ=255)            tGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGa                                                                                                                       <  2:214818/139‑1 (MQ=255)              cTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGaca                                                                                                                     >  2:1194916/1‑139 (MQ=255)                 cGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAAt                                                                                                                         <  2:471193/132‑1 (MQ=255)                 cGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAAt                                                                                                                         >  1:471193/1‑132 (MQ=255)                         cTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTa                                                                                                                            >  2:772619/1‑121 (MQ=255)                         cTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTa                                                                                                                            <  1:772619/121‑1 (MQ=255)                         cTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGa                                                                                                                       <  1:1145595/126‑1 (MQ=255)                         cTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGa                                                                                                                       >  2:1145595/1‑126 (MQ=255)                                 cgtcacaGGAGTTTCCGGTATTTCAGACGCGTTTGCTGCAAATGAAGCCAGCATGATAGCGTTCGCCGTTATTGTTTTATTCATTGTTACTGTCTCCTGTCTATTACAGTTTAATTGACACACCAATGTGATAACTGtt                                                                                                  <  1:524964/136‑1 (MQ=255)                                    cacaGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTc                                                                                               >  1:1008863/1‑139 (MQ=255)                                      caGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCg                                                                                             <  1:277679/139‑1 (MQ=255)                                       aGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                                            <  2:1008863/139‑1 (MQ=255)                                                    aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               <  2:919420/139‑1 (MQ=255)                                                    aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               <  1:69741/139‑1 (MQ=255)                                                    aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               <  1:1169978/139‑1 (MQ=255)                                                        cAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGt                                                                           >  1:40435/1‑139 (MQ=255)                                                           aCGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGt                                                                        >  2:656161/1‑139 (MQ=255)                                                            cgcgTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTa                                                                       >  1:863060/1‑139 (MQ=255)                                                             gcgTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               <  2:710679/130‑1 (MQ=255)                                                             gcgTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               >  1:710679/1‑130 (MQ=255)                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCGATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAaca                                                                   >  1:1532012/1‑139 (MQ=255)                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAaca                                                                   >  1:1240129/1‑139 (MQ=255)                                                                  tGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACAcc                                                                 <  1:1194916/139‑1 (MQ=255)                                                                  tGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACAcc                                                                 >  2:1259347/1‑139 (MQ=255)                                                                               gCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                    <  1:47324/139‑1 (MQ=255)                                                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCGATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                               <  2:1532012/139‑1 (MQ=255)                                                                                      tGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCat                                             <  2:232081/139‑1 (MQ=255)                                                                                         tAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATAtt                                          <  1:1259347/139‑1 (MQ=255)                                                                                         tAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTACCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATAtt                                          <  2:40435/139‑1 (MQ=255)                                                                                            cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               <  1:1457732/99‑1 (MQ=255)                                                                                            cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               >  2:1457732/1‑99 (MQ=255)                                                                                              aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatg                                     >  2:1138056/1‑139 (MQ=255)                                                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                                            >  1:1372523/1‑83 (MQ=255)                                                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                                            <  2:1372523/83‑1 (MQ=255)                                                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGcc                                                                             <  1:1474732/98‑1 (MQ=255)                                                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGcc                                                                             >  2:1474732/1‑98 (MQ=255)                                                                                                cgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatgta                                   >  2:465635/1‑139 (MQ=255)                                                                                                cgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatgta                                   <  2:1240129/139‑1 (MQ=255)                                                                                                               aTTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCTTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCggg                    <  1:1138056/139‑1 (MQ=255)                                                                                                               aTTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                               >  1:532225/1‑112 (MQ=255)                                                                                                               aTTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                               <  2:532225/112‑1 (MQ=255)                                                                                                                ttCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTaaa                                                                     <  2:1483316/89‑1 (MQ=255)                                                                                                                ttCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTaaa                                                                     >  1:1483316/1‑89 (MQ=255)                                                                                                                    ttGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCa               <  1:102165/139‑1 (MQ=255)                                                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCgg                     <  2:1324182/132‑1 (MQ=255)                                                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCgg                     >  1:1324182/1‑132 (MQ=255)                                                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCat              >  1:1520097/1‑139 (MQ=255)                                                                                                                         aCTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATgg          <  2:1520097/139‑1 (MQ=255)                                                                                                                          cTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCat                                             >  2:1124892/1‑103 (MQ=255)                                                                                                                          cTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCat                                             <  1:1124892/103‑1 (MQ=255)                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               >  2:1551627/1‑66 (MQ=255)                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                               <  1:1551627/66‑1 (MQ=255)                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGt                                                                        <  2:1313844/73‑1 (MQ=255)                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGt                                                                        >  1:1313844/1‑73 (MQ=255)                                                                                                                              ctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTg     >  1:890985/1‑139 (MQ=255)                                                                                                                                 cTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAg  <  2:37713/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATTGTAG  >  NC_000913/4538650‑4538916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑