Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,549,055 C→T S83L (TCG→TTG)  fimH → minor component of type 1 fimbriae

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,0550CT100.0% 228.4 / NA 70S83L (TCG→TTG) fimHminor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/39);  total (31/39)

GTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGG  >  NC_000913/4548928‑4549179
                                                                                                                               |                                                                                                                            
gTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATggcg                                                                                                                   <  1:575349/139‑1 (MQ=255)
     tGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCgtg                                                                                                              <  2:23789/139‑1 (MQ=255)
     tGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCgtg                                                                                                              <  2:698613/139‑1 (MQ=255)
          aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATc                                                                                                         >  2:1002027/1‑139 (MQ=255)
              tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAAt                                                                                                     >  2:1541303/1‑139 (MQ=255)
                      tcgtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                                                                                             <  1:1450044/139‑1 (MQ=255)
                        gtGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCCACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           <  1:1138680/139‑1 (MQ=255)
                           aaTGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCg                                                                                        <  2:652960/139‑1 (MQ=255)
                               tgGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTaaa                                                                                    >  1:589205/1‑139 (MQ=255)
                                 gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAAGGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAat                                                                                  >  1:1077378/1‑139 (MQ=255)
                                 gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                                                                                             <  1:777412/128‑1 (MQ=255)
                                 gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                                                                                             >  2:777412/1‑128 (MQ=255)
                                 gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           >  1:952762/1‑130 (MQ=255)
                                 gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           <  2:952762/130‑1 (MQ=255)
                                 gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                                                                                      >  1:1567938/1‑135 (MQ=255)
                                 gggCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTa                                                                                      <  2:1567938/135‑1 (MQ=255)
                                         ccTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACGATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTCATTTTTCCGGGACCGTAAAATATAGTGGc                                                                          <  2:1077378/139‑1 (MQ=255)
                                           tGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAg                                                                        <  2:589205/139‑1 (MQ=255)
                                            ggTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATggc                                                                                                                    >  2:704558/1‑94 (MQ=255)
                                            ggTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATggc                                                                                                                    <  1:704558/94‑1 (MQ=255)
                                            ggTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           >  2:120771/1‑119 (MQ=255)
                                            ggTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           <  1:120771/119‑1 (MQ=255)
                                              tCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGtagtag                                                                     <  1:852786/139‑1 (MQ=255)
                                                      cTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAt                                                             <  2:1240032/139‑1 (MQ=255)
                                                      cTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAt                                                             <  2:167649/139‑1 (MQ=255)
                                                           gACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCt                                                        >  2:1546112/1‑139 (MQ=255)
                                                            aCGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATc                                                                >  2:94774/1‑130 (MQ=255)
                                                            aCGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATc                                                                <  1:94774/130‑1 (MQ=255)
                                                              gCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAggcttggctt                                                                                                                         >  1:630008/1‑71 (MQ=255)
                                                              gCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAggcttggctt                                                                                                                         <  2:630008/71‑1 (MQ=255)
                                                              gCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTacc                                                     <  2:1345474/139‑1 (MQ=255)
                                                                aaaTCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTTGCTATCCATTTCCTaccac                                                   <  1:1002027/139‑1 (MQ=255)
                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATggcg                                                                                                                   <  1:951516/61‑1 (MQ=255)
                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATggcg                                                                                                                   >  2:951516/1‑61 (MQ=255)
                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                                                                                             <  2:1473207/83‑1 (MQ=255)
                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                                                                                             >  1:1473207/1‑83 (MQ=255)
                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           >  2:119412/1‑85 (MQ=255)
                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           <  1:119412/85‑1 (MQ=255)
                                                                              aaCGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAAcgccgc                                     >  1:1004054/1‑139 (MQ=255)
                                                                                 gATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgcg                                  <  1:1546112/139‑1 (MQ=255)
                                                                                 gATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCcgcgcg                                  <  2:970111/139‑1 (MQ=255)
                                                                                       ccGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                     <  1:1529813/98‑1 (MQ=255)
                                                                                       ccGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                     >  2:1529813/1‑98 (MQ=255)
                                                                                         ggAAACCATTACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTtat                          <  1:1541303/139‑1 (MQ=255)
                                                                                                 ttACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCg                                                                                        >  1:1229651/1‑69 (MQ=255)
                                                                                                 ttACAGACTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCg                                                                                        <  2:1229651/69‑1 (MQ=255)
                                                                                                       aCTATGTCACACTGCTACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACACCGCGAGTTGTTTATTATTCGAGAACGGa            >  1:776446/1‑139 (MQ=255)
                                                                                                       aCTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCa                                                                         >  1:73222/1‑78 (MQ=255)
                                                                                                       aCTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCa                                                                         <  2:73222/78‑1 (MQ=255)
                                                                                                       aCTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGaa                >  2:322757/1‑135 (MQ=255)
                                                                                                       aCTATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGaa                <  1:322757/135‑1 (MQ=255)
                                                                                                         tATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAAt                       >  1:925981/1‑126 (MQ=255)
                                                                                                         tATGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAAt                       <  2:925981/126‑1 (MQ=255)
                                                                                                           tGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           <  2:1192932/56‑1 (MQ=255)
                                                                                                           tGTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           >  1:1192932/1‑56 (MQ=255)
                                                                                                            gTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAAt                                                                                                     >  1:619257/1‑45 (MQ=255)
                                                                                                            gTCACACTGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAAt                                                                                                     <  2:619257/45‑1 (MQ=255)
                                                                                                                 aatGCAACCAGGCTTGGCATATAGCGACGAGTTATGTAATTTTTCAGGGACCGTAAAACATAATGGCAGTAGCTATCAATTACCTACCACCAGCGAGACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTgg  >  1:1602186/3‑139 (MQ=255)
                                                                                                                   tGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           >  1:707707/1‑48 (MQ=255)
                                                                                                                   tGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           <  2:707707/48‑1 (MQ=255)
                                                                                                                   tGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                     >  2:1413006/1‑70 (MQ=255)
                                                                                                                   tGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                     <  1:1413006/70‑1 (MQ=255)
                                                                                                                   tGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGaa                <  2:1349087/123‑1 (MQ=255)
                                                                                                                   tGCAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGaa                >  1:1349087/1‑123 (MQ=255)
                                                                                                                     cAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGc                                                                          <  1:692766/63‑1 (MQ=255)
                                                                                                                     cAACGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGc                                                                          >  2:692766/1‑63 (MQ=255)
                                                                                                                      aaCGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGc                                               >  1:1472833/1‑89 (MQ=255)
                                                                                                                      aaCGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGc                                               <  2:1472833/89‑1 (MQ=255)
                                                                                                                       aCGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAt                                                             <  1:1536543/74‑1 (MQ=255)
                                                                                                                       aCGAGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCAt                                                             >  2:1536543/1‑74 (MQ=255)
                                                                                                                          aGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           >  2:937977/1‑41 (MQ=255)
                                                                                                                          aGGCTTGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                                                                                           <  1:937977/41‑1 (MQ=255)
                                                                                                                           ggcttggcttATGTCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATGTAGTGGCAGTAg                                                                     >  1:1170319/1‑62 (MQ=255)
                                                                                                                           ggcttggcttATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg                                                                     <  2:1170319/62‑1 (MQ=255)
                                                                                                                               |                                                                                                                            
GTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGG  >  NC_000913/4548928‑4549179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: