Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	379,606	C→T	*92* (TAG→TAA)	frmR ←	regulator protein that represses frmRAB operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	379,606	0	C	T	100.0%	167.1 / NA	52	*92* (TAG→TAA)	frmR	regulator protein that represses frmRAB operon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/26);  total (26/26)

CTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCG  >  NC_000913/379472‑379739                                                                                                                                       |                                                                                                                                      cTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTAtt                                                                                                                                   <  2:120382/139‑1 (MQ=255) cTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACATGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTAtt                                                                                                                                   >  2:1346595/1‑139 (MQ=255)  tttAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTAttt                                                                                                                                  <  1:1210919/139‑1 (MQ=255)    tAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTaa                                                                                                                                >  1:1245741/1‑139 (MQ=255)            aCTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCa                                                                                                                        >  1:707781/1‑139 (MQ=255)            aCTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCa                                                                                                                        <  1:1262779/139‑1 (MQ=255)              ttCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCa                                                                                                                        >  2:240806/1‑137 (MQ=255)              ttCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCa                                                                                                                        <  1:240806/137‑1 (MQ=255)               tCACATTTTTTCGGTGGTGCAACGTCAATTTCAACGATTCCCAGCGGTTGACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTGTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGa                                                                                                                     <  1:876697/139‑1 (MQ=255)                cACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCGTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGaa                                                                                                                    >  1:965590/1‑139 (MQ=255)                cACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGaa                                                                                                                    >  2:207624/1‑139 (MQ=255)                 aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                   <  1:1434404/139‑1 (MQ=255)                 aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                   <  2:1245741/139‑1 (MQ=255)                 aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                   <  1:740863/139‑1 (MQ=255)                 aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                   <  1:207624/139‑1 (MQ=255)                           ggtggtGCAACGTCAATTTCAACGATTTTCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATa                                                                                                         <  2:965590/139‑1 (MQ=255)                                tgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTgtc                                                                                                    >  2:912518/1‑139 (MQ=255)                                        cAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACgg                                                                                            <  2:707781/139‑1 (MQ=255)                                                     ttCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTc                                                                               <  2:97402/139‑1 (MQ=255)                                                             gTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGt                                                                       >  2:1172069/1‑139 (MQ=255)                                                             gTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGt                                                                       <  2:1217917/139‑1 (MQ=255)                                                                       aGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGTGGCTGTAGCAGTCAtt                                                             >  2:1424897/1‑139 (MQ=255)                                                                                 aCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGAt                                                                                                                             >  2:1449346/1‑65 (MQ=255)                                                                                 aCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGAt                                                                                                                             <  1:1449346/65‑1 (MQ=255)                                                                                 aCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGGTTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAAc                                                   >  1:1015104/1‑139 (MQ=255)                                                                                               tttCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATg                                     <  2:1015104/139‑1 (MQ=255)                                                                                                  cATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                   <  2:1604465/58‑1 (MQ=255)                                                                                                  cATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                   >  1:1604465/1‑58 (MQ=255)                                                                                                       ctcGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCaa                             >  1:1584189/1‑139 (MQ=255)                                                                                                        tcGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACtt                                                                                <  1:342882/87‑1 (MQ=255)                                                                                                        tcGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACtt                                                                                >  2:342882/1‑87 (MQ=255)                                                                                                                  tCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGTGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGcc                  <  1:1424897/139‑1 (MQ=255)                                                                                                                      tatGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCTACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCaa                             <  2:1003709/124‑1 (MQ=255)                                                                                                                      tatGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCaa                             >  1:1003709/1‑124 (MQ=255)                                                                                                                        tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                             >  2:755614/1‑74 (MQ=255)                                                                                                                        tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                             <  1:755614/74‑1 (MQ=255)                                                                                                                        tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGc            >  2:1602601/1‑139 (MQ=255)                                                                                                                        tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGc            >  1:1189177/1‑139 (MQ=255)                                                                                                                          gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                             >  1:1171775/1‑72 (MQ=255)                                                                                                                          gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                             <  2:1171775/72‑1 (MQ=255)                                                                                                                          gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGt                                                        >  2:1220165/1‑93 (MQ=255)                                                                                                                          gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGt                                                        <  1:1220165/93‑1 (MQ=255)                                                                                                                           tAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAgtgt                                                                                                     <  2:1606834/47‑1 (MQ=255)                                                                                                                           tAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAgtgt                                                                                                     >  1:1606834/1‑47 (MQ=255)                                                                                                                           tAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCa         >  1:354442/1‑139 (MQ=255)                                                                                                                              tAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTa      >  1:1450325/1‑139 (MQ=255)                                                                                                                                 aTTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGcc   <  1:1172069/139‑1 (MQ=255)                                                                                                                                  ttCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCcg  >  2:1191439/1‑139 (MQ=255)                                                                                                                                   tCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTc                                              <  1:797691/94‑1 (MQ=255)                                                                                                                                   tCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTc                                              >  2:797691/1‑94 (MQ=255)                                                                                                                                    cAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCaa                                                     >  2:32496/1‑86 (MQ=255)                                                                                                                                    cAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCaa                                                     <  1:32496/86‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCG  >  NC_000913/379472‑379739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑