Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	411,297	C→T	*395* (TAG→TAA)	araJ ←	L‑arabinose‑inducible putative transporter, MFS family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	411,297	0	C	T	100.0%	148.5 / NA	46	*395* (TAG→TAA)	araJ	L‑arabinose‑inducible putative transporter, MFS family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/21);  total (25/21)

ATAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCC  >  NC_000913/411166‑411432                                                                                                                                    |                                                                                                                                        aTAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCa                                                                                                                                  <  2:719924/139‑1 (MQ=255)         tCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCa                                                                                                                                  >  2:612556/1‑131 (MQ=255)         tCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCa                                                                                                                                  <  1:612556/131‑1 (MQ=255)                ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCa                                                                                                                   >  2:1554170/1‑139 (MQ=255)                ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCa                                                                                                                   >  2:1182547/1‑139 (MQ=255)                         aCGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAg                                                                                                             <  1:120811/136‑1 (MQ=255)                         aCGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAg                                                                                                             >  2:120811/1‑136 (MQ=255)                          cGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATc                                                                                                         >  2:1209121/1‑139 (MQ=255)                           gCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTAc                                                                                                                                      >  1:1034338/1‑109 (MQ=255)                           gCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTAc                                                                                                                                      <  2:1034338/109‑1 (MQ=255)                                         aTCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGgcg                                                                                          <  1:1033363/139‑1 (MQ=255)                                           cAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCt                                                                                        <  1:1209121/139‑1 (MQ=255)                                              gCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTtat                                                                                     <  1:1030268/139‑1 (MQ=255)                                                cTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAg                                                                                   >  1:440578/1‑139 (MQ=255)                                                 tGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                                  <  1:1086736/139‑1 (MQ=255)                                                 tGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                                  >  1:741265/1‑139 (MQ=255)                                                  gCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                                  <  2:744686/138‑1 (MQ=255)                                                  gCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                                  >  1:744686/1‑138 (MQ=255)                                                  gCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCg                                                                                 >  2:715413/1‑139 (MQ=255)                                                   cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGa                                                                                >  2:590715/1‑139 (MQ=255)                                                    aaaaTGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGAc                                                                               >  1:1303167/1‑139 (MQ=255)                                                           gCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTtat                                                                                     >  1:350351/1‑126 (MQ=255)                                                           gCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTtat                                                                                     <  2:350351/126‑1 (MQ=255)                                                                          ggCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACAt                                                         <  1:590715/139‑1 (MQ=255)                                                                            cGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCg                                                       >  1:492180/1‑139 (MQ=255)                                                                                    gCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAAc                                               >  1:177090/1‑139 (MQ=255)                                                                                        tCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGaaa                                           >  1:804726/1‑139 (MQ=255)                                                                                         cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAg                                          <  2:1303167/139‑1 (MQ=255)                                                                                         cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTA‑CCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAg                                          <  2:367407/138‑1 (MQ=255)                                                                                         cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTA‑CCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAg                                          >  1:367407/1‑138 (MQ=255)                                                                                              cgcATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAggg                                     >  1:896424/1‑139 (MQ=255)                                                                                                          tAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcg                         <  2:440578/139‑1 (MQ=255)                                                                                                             gTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATccgc                                                                                                      >  2:611691/1‑59 (MQ=255)                                                                                                             gTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATccgc                                                                                                      <  1:611691/59‑1 (MQ=255)                                                                                                               aaTGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACg                    <  2:413574/139‑1 (MQ=255)                                                                                                               aaTGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACg                    <  2:492180/139‑1 (MQ=255)                                                                                                                aTGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGt                   >  2:806545/1‑139 (MQ=255)                                                                                                                     cACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATta              >  1:694935/1‑139 (MQ=255)                                                                                                                     cACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATta              >  1:209296/1‑139 (MQ=255)                                                                                                                          agaCTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGgcg                                                                                          >  1:729044/1‑58 (MQ=255)                                                                                                                          agaCTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGgcg                                                                                          <  2:729044/58‑1 (MQ=255)                                                                                                                               ataACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcg                         >  2:36652/1‑118 (MQ=255)                                                                                                                               ataACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcg                         <  1:36652/118‑1 (MQ=255)                                                                                                                                taACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                        <  1:742961/102‑1 (MQ=255)                                                                                                                                taACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                        >  2:742961/1‑102 (MQ=255)                                                                                                                                 aaCTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGcccc  <  2:741265/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        ATAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCC  >  NC_000913/411166‑411432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑