Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	437,107	G→A	*173* (TAG→TAA)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	437,107	0	G	A	100.0%	134.3 / NA	42	*173* (TAG→TAA)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/24);  total (18/24)

TGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCAC  >  NC_000913/436973‑437241                                                                                                                                       |                                                                                                                                       tGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgt                                                                                                                                    >  2:1206992/1‑139 (MQ=255) tGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgt                                                                                                                                    >  2:432296/1‑139 (MQ=255)    ccGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCa                                                                                                                                 <  2:461518/139‑1 (MQ=255)                      cGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACg                                                                                                               <  1:432296/139‑1 (MQ=255)                      cGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACg                                                                                                               <  1:51230/139‑1 (MQ=255)                                  ggcggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGtct                                                                                                   >  2:742166/1‑139 (MQ=255)                                     ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  2:172524/139‑1 (MQ=255)                                     ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  2:18133/139‑1 (MQ=255)                                     ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  1:424143/139‑1 (MQ=255)                                     ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  1:682871/139‑1 (MQ=255)                                        aTGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAg                                                                                             <  2:1019416/139‑1 (MQ=255)                                                  tGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGActc                                                                                   <  1:1206992/139‑1 (MQ=255)                                                     tCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                                                                                    <  1:541261/135‑1 (MQ=255)                                                     tCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                                                                                    >  2:541261/1‑135 (MQ=255)                                                     tCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTa                                                                                >  2:290486/1‑139 (MQ=255)                                                               tGTCTCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                     <  2:974238/108‑1 (MQ=255)                                                               tGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                     >  1:974238/1‑108 (MQ=255)                                                               tGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCt                                                                      <  1:9525/139‑1 (MQ=255)                                                                   gCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGa                                                                  >  1:830467/1‑139 (MQ=255)                                                                     cGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACa                                                                <  2:279203/139‑1 (MQ=255)                                                                          tGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATg                                                           <  1:290486/139‑1 (MQ=255)                                                                                   cAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                            <  1:975582/113‑1 (MQ=255)                                                                                   cAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                            >  2:975582/1‑113 (MQ=255)                                                                                      gCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACgg                                               <  2:830467/139‑1 (MQ=255)                                                                                                     tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGActc                                                                                   >  1:154308/1‑88 (MQ=255)                                                                                                     tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGActc                                                                                   <  2:154308/88‑1 (MQ=255)                                                                                                     tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCgg                                                        >  2:981584/1‑115 (MQ=255)                                                                                                     tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCgg                                                        <  1:981584/115‑1 (MQ=255)                                                                                                              aCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTc                       <  2:515050/139‑1 (MQ=255)                                                                                                                   ccGCTGGGTATTCTGTCGTAATTGTGCACCGATGc                                                                                                                          <  2:1264785/35‑1 (MQ=255)                                                                                                                   ccGCTGGGTATTCTGTCGTAATTGTGCACCGATGc                                                                                                                          >  1:1264785/1‑35 (MQ=38)                                                                                                                   ccGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCt                  >  2:431089/1‑139 (MQ=255)                                                                                                                       tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  1:699303/57‑1 (MQ=255)                                                                                                                       tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                >  2:699303/1‑57 (MQ=255)                                                                                                                       tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGAt                                                            >  2:1382615/1‑93 (MQ=255)                                                                                                                       tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGAt                                                            <  1:1382615/93‑1 (MQ=255)                                                                                                                       tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTta                                                    <  1:1138934/101‑1 (MQ=255)                                                                                                                       tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTta                                                    >  2:1138934/1‑101 (MQ=255)                                                                                                                       tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCaa              >  1:726191/1‑139 (MQ=255)                                                                                                                                   cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCaaa             <  2:1156064/128‑1 (MQ=255)                                                                                                                                   cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCaaa             >  1:1156064/1‑128 (MQ=255)                                                                                                                                   cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCAc  >  2:516149/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCAC  >  NC_000913/436973‑437241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑