Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,510	A→G	E43G (GAG→GGG)	ybaO →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,510	0	A	G	100.0%	179.0 / NA	53	E43G (GAG→GGG)	ybaO	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/24);  total (29/24)

GAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCG  >  NC_000913/468375‑468627                                                                                                                                        |                                                                                                                      gAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGTACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                    <  2:673517/139‑1 (MQ=255)  aaGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgac                                                                                                                   <  2:1179057/139‑1 (MQ=255)         aTGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                            >  1:1110257/1‑139 (MQ=255)         aTGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                            >  1:1545582/1‑139 (MQ=255)                aTAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTAt                                                                                                     >  2:914402/1‑139 (MQ=255)                aTAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGACTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTAt                                                                                                     >  2:1305439/1‑139 (MQ=255)                    aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGc                                                                                                 >  1:902380/1‑139 (MQ=255)                         aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGt                                                                                            >  2:621609/1‑139 (MQ=255)                           cGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                          <  1:185868/139‑1 (MQ=255)                                                 agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAAt                                                                    >  1:727970/1‑139 (MQ=255)                                                   cagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGTAGCGACTGAAACGGCTGGGGGACGACGGTATGCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAg                                                                  >  1:463224/1‑139 (MQ=255)                                                     ggATTGCACCCTCTCTTTGCAGGCACTGACTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGc                                                                <  1:1305439/139‑1 (MQ=255)                                                      gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGcc                                                               >  2:809672/1‑139 (MQ=255)                                                           cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                 >  1:704643/1‑84 (MQ=255)                                                           cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                 <  2:704643/84‑1 (MQ=255)                                                           cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGc                                                          >  2:292043/1‑139 (MQ=255)                                                             cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                    >  2:1433348/1‑79 (MQ=255)                                                             cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                    <  1:1433348/79‑1 (MQ=255)                                                                  tctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGcc                                                                                        >  2:568/1‑102 (MQ=255)                                                                  tctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGcc                                                                                        <  1:568/102‑1 (MQ=255)                                                                          ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGc                                                                                         >  2:1542871/1‑93 (MQ=255)                                                                          ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGc                                                                                         <  1:1542871/93‑1 (MQ=255)                                                                          ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTAGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtgtg                                           >  2:1028634/1‑139 (MQ=255)                                                                            cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt                                         <  2:727970/139‑1 (MQ=255)                                                                              cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                               <  1:130915/99‑1 (MQ=255)                                                                              cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                               >  2:130915/1‑99 (MQ=255)                                                                                    gAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCTCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTAGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATaaaaa                                 <  1:1028634/139‑1 (MQ=255)                                                                                     aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAAc                                >  1:654260/1‑139 (MQ=255)                                                                                      aGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACg                               <  1:292043/139‑1 (MQ=255)                                                                                       gCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGc                              <  1:259803/139‑1 (MQ=255)                                                                                           ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                            >  1:1441838/1‑57 (MQ=255)                                                                                           ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                            <  2:1441838/57‑1 (MQ=255)                                                                                           ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCt                                                              <  1:192031/103‑1 (MQ=255)                                                                                           ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCt                                                              >  2:192031/1‑103 (MQ=255)                                                                                            tAATCTGACGACCCCCCCTGGCTGGAAGCGCCTAAAACGGCTGGGGGACGACGCTAGCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGTAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACAt                         <  1:187634/139‑1 (MQ=255)                                                                                              aTCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATca                       <  2:654260/139‑1 (MQ=255)                                                                                                   aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcagca                  <  1:809672/139‑1 (MQ=255)                                                                                                      accaccCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCg               <  2:1545582/139‑1 (MQ=255)                                                                                                        caccCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCctgct                                                                                   <  2:152118/69‑1 (MQ=255)                                                                                                        caccCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCctgct                                                                                   >  1:152118/1‑69 (MQ=255)                                                                                                          ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATg           >  1:1499846/1‑139 (MQ=255)                                                                                                             cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCaa                                                                                               <  2:504345/52‑1 (MQ=255)                                                                                                             cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCaa                                                                                               >  1:504345/1‑52 (MQ=255)                                                                                                               tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCtt                                                <  2:1396090/97‑1 (MQ=255)                                                                                                               tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCtt                                                >  1:1396090/1‑97 (MQ=255)                                                                                                               tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTAtt      >  1:160075/1‑139 (MQ=255)                                                                                                               tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTAtt      >  2:260164/1‑139 (MQ=255)                                                                                                               tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTAtt      >  1:457271/1‑139 (MQ=255)                                                                                                                   ggAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCg  >  2:147425/1‑139 (MQ=255)                                                                                                                   ggAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCg  <  2:902380/139‑1 (MQ=255)                                                                                                                     aaGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                               <  2:98040/60‑1 (MQ=255)                                                                                                                     aaGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                               >  1:98040/1‑60 (MQ=255)                                                                                                                       gcgcCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGc                              <  2:1612241/107‑1 (MQ=255)                                                                                                                       gcgcCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCCTTTGTGCTGATAAAAACGc                              >  1:1612241/1‑107 (MQ=255)                                                                                                                          ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCTcagca                  <  1:906682/116‑1 (MQ=255)                                                                                                                          ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcagca                  >  2:906682/1‑116 (MQ=255)                                                                                                                                        |                                                                                                                      GAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCG  >  NC_000913/468375‑468627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑