Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	477,435	T→C	T395A (ACT→GCT)	ylaB ←	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	477,435	0	T	C	100.0%	261.0 / NA	73	T395A (ACT→GCT)	ylaB	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/36);  total (37/36)

CGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTG  >  NC_000913/477300‑477555                                                                                                                                        |                                                                                                                         cGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAg                                                                                                                       >  2:13740/1‑139 (MQ=255)   aCATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt                                                                                                                     >  2:1509412/1‑139 (MQ=255)             ccTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  2:572807/139‑1 (MQ=255)             ccTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  1:29235/139‑1 (MQ=255)             ccTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  1:13740/139‑1 (MQ=255)                gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctg                                                                                                        >  1:1495962/1‑139 (MQ=255)                 tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGACAAACTCGCGTTCAGCGAGtt                                                                                                                     >  2:287308/1‑125 (MQ=255)                 tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCACCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt                                                                                                                     <  1:287308/125‑1 (MQ=255)                  aaaTAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc                                                                                                      <  2:1446483/139‑1 (MQ=255)                           aaaCTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTaa                                                                                             >  1:160978/1‑139 (MQ=255)                            aaCTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAAc                                                                                            <  2:160978/139‑1 (MQ=255)                                  aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  1:445849/118‑1 (MQ=255)                                  aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           >  2:445849/1‑118 (MQ=255)                                  aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                        >  2:1286189/1‑137 (MQ=255)                                  aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                        <  1:1286189/137‑1 (MQ=255)                                         cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgattg                                                                               >  1:1498961/1‑139 (MQ=255)                                         cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgattg                                                                               >  1:78248/1‑139 (MQ=255)                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAgctgtctct                                                                             >  1:1114350/1‑131 (MQ=255)                                              cAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  1:399174/106‑1 (MQ=255)                                              cAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           >  2:399174/1‑106 (MQ=255)                                                aaaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAgctgtctcttatac                                                                        <  2:1114350/139‑14 (MQ=255)                                                aaaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGt                                                                        >  1:1210900/1‑139 (MQ=255)                                                 aaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAAc                                                                                            >  2:1185504/1‑118 (MQ=255)                                                 aaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAAc                                                                                            <  1:1185504/118‑1 (MQ=255)                                                     atcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           >  2:9542/1‑99 (MQ=255)                                                     atcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  1:9542/99‑1 (MQ=255)                                                     atcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACgc                                                                   >  2:1567826/1‑139 (MQ=255)                                                      tcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGt                                                                        <  1:168484/133‑1 (MQ=255)                                                      tcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGt                                                                        >  2:168484/1‑133 (MQ=255)                                                                    aTGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCa                                                                      <  1:50760/121‑1 (MQ=255)                                                                    aTGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCa                                                                      >  2:50760/1‑121 (MQ=255)                                                                     tGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                >  2:1463710/1‑94 (MQ=255)                                                                     tGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                <  1:1463710/94‑1 (MQ=255)                                                                          cGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAg                                                                                                                 >  2:959670/1‑72 (MQ=255)                                                                          cGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAg                                                                                                                 <  1:959670/72‑1 (MQ=255)                                                                             cTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAATTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg                                           <  2:543110/139‑1 (MQ=255)                                                                             cTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTACAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGACACGCAGCAATTGCGGGATTTTTTCCGAg                                           <  2:1077266/139‑1 (MQ=255)                                                                                  ggTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  2:1405030/70‑1 (MQ=255)                                                                                  ggTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           >  1:1405030/1‑70 (MQ=255)                                                                                  ggTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGa                                                                                                 >  2:1313872/1‑80 (MQ=255)                                                                                  ggTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGa                                                                                                 <  1:1313872/80‑1 (MQ=255)                                                                                  ggTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAg                                      >  2:1364946/1‑139 (MQ=255)                                                                                      gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACg                                  >  1:4778/1‑139 (MQ=255)                                                                                      gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACg                                  >  1:1106586/1‑139 (MQ=255)                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg                                 >  2:1058330/1‑139 (MQ=255)                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg                                 >  1:526401/1‑139 (MQ=255)                                                                                        gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCgg                                                        <  1:350056/115‑1 (MQ=255)                                                                                        gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCgg                                                        >  2:350056/1‑115 (MQ=255)                                                                                        gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg                                <  2:377890/139‑1 (MQ=255)                                                                                        gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg                                <  2:78248/139‑1 (MQ=255)                                                                                        gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg                                <  1:1364946/139‑1 (MQ=255)                                                                                         agaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  1:1509759/63‑1 (MQ=255)                                                                                         agaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           >  2:1509759/1‑63 (MQ=255)                                                                                            aaTTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGAt                            >  2:1225343/1‑139 (MQ=255)                                                                                                 tCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAg                       <  2:1498961/139‑1 (MQ=255)                                                                                                  cGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGa                      <  2:1106586/139‑1 (MQ=255)                                                                                                      gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                       >  1:421193/1‑70 (MQ=255)                                                                                                      gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                       <  2:421193/70‑1 (MQ=255)                                                                                                      gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGa                  <  2:1495962/139‑1 (MQ=255)                                                                                                       cgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGAt                 >  2:269717/1‑139 (MQ=255)                                                                                                        gcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           >  2:689826/1‑48 (MQ=255)                                                                                                        gcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  1:689826/48‑1 (MQ=255)                                                                                                           ggTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGc                                                          <  1:1025011/94‑1 (MQ=255)                                                                                                           ggTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGc                                                          >  2:1025011/1‑94 (MQ=255)                                                                                                           ggTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGaa             <  1:1058330/139‑1 (MQ=255)                                                                                                              tttCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAata          <  2:1210900/139‑1 (MQ=255)                                                                                                                    aTCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           <  2:393531/36‑1 (MQ=255)                                                                                                                    aTCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                           >  1:393531/1‑36 (MQ=255)                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                        >  1:1593776/1‑53 (MQ=255)                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                        <  2:1593776/53‑1 (MQ=255)                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGctgctg  >  2:423225/1‑139 (MQ=255)                                                                                                                         cAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACg                                                                    <  1:831062/70‑1 (MQ=255)                                                                                                                         cAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACg                                                                    >  2:831062/1‑70 (MQ=255)                                                                                                                                        |                                                                                                                         CGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTG  >  NC_000913/477300‑477555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑