Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,334	C→T	*125* (TAG→TAA)	tomB ←	Hha toxicity attenuator, conjugation‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,334	0	C	T	100.0%	246.8 / NA	75	*125* (TAG→TAA)	tomB	Hha toxicity attenuator, conjugation‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/39);  total (36/39)

TCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATC  >  NC_000913/480199‑480468                                                                                                                                        |                                                                                                                                       tCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                     <  2:288368/139‑1 (MQ=255)    gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                     <  1:810240/136‑1 (MQ=255)    gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                     >  2:810240/1‑136 (MQ=255)    gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACaa                                                                                                                                  >  1:947324/1‑139 (MQ=255)            gTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACt                                                                                                                          <  1:585388/139‑1 (MQ=255)             tatttattTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTc                                                                                                                         >  1:1158457/1‑139 (MQ=255)              atttatttTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCg                                                                                                                        <  1:328381/139‑1 (MQ=255)                                cacGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTtcgtcg                                                                                                      >  2:550055/1‑139 (MQ=255)                                    cTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCAtt                                                                                                  <  1:626600/139‑1 (MQ=255)                                     tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTg                                                                                                 >  2:870231/1‑139 (MQ=255)                                         gCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                             <  2:1279761/139‑1 (MQ=255)                                         gCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                             <  2:1158457/139‑1 (MQ=255)                                              tCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                        >  2:197431/1‑139 (MQ=255)                                               cAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAAc                                                                                                                                    >  2:602126/1‑94 (MQ=255)                                               cAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAAc                                                                                                                                    <  1:602126/94‑1 (MQ=255)                                                        ggCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                     <  1:193225/116‑1 (MQ=255)                                                        ggCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                     >  2:193225/1‑116 (MQ=255)                                                        ggCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTc                                                                              >  2:949133/1‑139 (MQ=255)                                                                        cATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCg                                                              >  1:1171201/1‑139 (MQ=255)                                                                          ttAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGcc                                                            >  2:760219/1‑139 (MQ=255)                                                                           tAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                           <  1:197431/139‑1 (MQ=255)                                                                                 atCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                        <  2:938073/104‑1 (MQ=255)                                                                                 atCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                        >  1:938073/1‑104 (MQ=255)                                                                                 atCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTg                                                     >  2:333012/1‑139 (MQ=255)                                                                                  tCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAcc                                                                       <  2:364759/120‑1 (MQ=255)                                                                                  tCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAcc                                                                       >  1:364759/1‑120 (MQ=255)                                                                                  tCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                             <  2:14711/130‑1 (MQ=255)                                                                                  tCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                             >  1:14711/1‑130 (MQ=255)                                                                                  tCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGa                                                    <  1:333012/139‑1 (MQ=255)                                                                                   cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                             >  2:31006/1‑129 (MQ=255)                                                                                   cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                             <  1:31006/129‑1 (MQ=255)                                                                                        ttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATcc                                              <  1:23831/139‑1 (MQ=255)                                                                                        ttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATcc                                              <  1:949133/139‑1 (MQ=255)                                                                                          cGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            <  2:1171201/139‑1 (MQ=255)                                                                                          cGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            <  1:1158213/139‑1 (MQ=255)                                                                                          cGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            <  2:1419686/139‑1 (MQ=255)                                                                                                aGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTAGTCGCATTGAAAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                      <  2:1388966/139‑1 (MQ=255)                                                                                                        cGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                              <  1:1369829/139‑1 (MQ=255)                                                                                                             aTACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCGGGAGATCCTGCATATTAATACCATAacta                         <  1:760219/139‑1 (MQ=255)                                                                                                                     aCCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaacaa                 >  1:665820/1‑139 (MQ=255)                                                                                                                       cTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                           <  1:1253766/95‑1 (MQ=255)                                                                                                                       cTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                           >  2:1253766/1‑95 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                             >  1:255427/1‑59 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                             <  2:255427/59‑1 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGa                                                    <  1:612915/100‑1 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGa                                                    >  2:612915/1‑100 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            <  2:614564/108‑1 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            >  1:614564/1‑108 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            <  2:566019/108‑1 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            >  1:566019/1‑108 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                      >  1:699650/1‑114 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                      <  2:699650/114‑1 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                              >  2:1426668/1‑122 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                              <  1:1426668/122‑1 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAacta                         >  2:333780/1‑127 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAacta                         <  1:333780/127‑1 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCACCATTTCTGa                                                    >  2:1201900/1‑100 (MQ=255)                                                                                                                         aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCACCATTTCTGa                                                    <  1:1201900/100‑1 (MQ=255)                                                                                                                          tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAacta                         <  2:957608/126‑1 (MQ=255)                                                                                                                          tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAacta                         >  1:957608/1‑126 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATATACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            <  1:950955/106‑1 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGa                                                 >  2:1136503/1‑101 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGa                                                 <  1:1136503/101‑1 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                            >  2:950955/1‑106 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaacaa                 >  2:1464818/1‑133 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaacaa                 <  1:1464818/133‑1 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaaa           >  2:649134/1‑139 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaaa           >  1:1615868/1‑139 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaaa           >  2:82150/1‑139 (MQ=255)                                                                                                                           ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaaa           >  1:1373307/1‑139 (MQ=255)                                                                                                                              tGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                    <  2:1232411/127‑1 (MQ=255)                                                                                                                              tGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                    >  1:1232411/1‑127 (MQ=255)                                                                                                                               gTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtg       <  1:550055/139‑1 (MQ=255)                                                                                                                                taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtgt      >  1:1195132/1‑139 (MQ=255)                                                                                                                                    aatTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATc  <  2:947324/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATC  >  NC_000913/480199‑480468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑