Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 357,626 2 bp→TA intergenic (+172/+164) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,6260CT100.0% 30.4 / NA 15intergenic (+172/+165)codA/cynRcytosine/isoguanine deaminase/transcriptional activator of cyn operon, autorepressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/6);  total (9/6)
*NC_000913357,6270CA93.3% 28.6 / ‑5.9 15intergenic (+173/+164)codA/cynRcytosine/isoguanine deaminase/transcriptional activator of cyn operon, autorepressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (9/5);  minor base T (0/1);  total (9/6)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCA  >  NC_000913/357548‑357756
                                                                              ||                                                                                                                                 
cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTg                                                                                   >  1:1233836/1‑128 (MQ=11)
cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTg                                                                                   <  2:1233836/128‑1 (MQ=1)
cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGcc                                                                        >  1:65965/1‑139 (MQ=255)
cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGcc                                                                        >  2:1416697/1‑139 (MQ=255)
             cgtaTTCCGGACGATGATCTGTATCAGCGCTTCCTGTGATTCATTACCGGCCACATCCGTGCATTTTTACAACGCCATGTGCAAAAAGGCCTTCGCAGCGGCTTTCTGCCAGCTGTAGTTTATGCCGTATGCGGCGTgc                                                           <  1:578074/136‑2 (MQ=12)
                         gCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTATTTTATCCGCGACGCGGCGTGAACGCCTTATcgt                                               >  1:1461056/1‑137 (MQ=14)
                                  tgtCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCATCGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                      <  2:765947/139‑1 (MQ=18)
                                  tgtCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                      >  1:1000195/1‑139 (MQ=18)
                                  tgtCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                      >  1:1067287/1‑139 (MQ=18)
                                   gtCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTag                                     <  1:589129/139‑1 (MQ=255)
                                            tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                            <  1:798362/107‑1 (MQ=255)
                                            tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                            >  2:798362/1‑107 (MQ=14)
                                             aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCATTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAAc                           >  2:1407775/1‑139 (MQ=255)
                                                              ccAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGc          >  1:758316/1‑139 (MQ=255)
                                                                      cgcgCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGCATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCa  <  2:1000195/139‑1 (MQ=255)
                                                                              ||                                                                                                                                 
CGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCA  >  NC_000913/357548‑357756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑