Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,370,0360T.100.0% 123.6 / NA 30intergenic (+33/‑180)pspE/ycjMthiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/15);  total (15/15)

AAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAC  >  NC_000913/1369912‑1370156
                                                                                                                            |                                                                                                                        
aagcaaAAGAGAGCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGGCAAAGGTTAAAAGAATTCACCATGAGCGGCGATTATGCGCCGTTTTTTTTCTGTcac                                                                                                           <  2:41982/139‑1 (MQ=255)
          gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTa                                                                                                 <  1:563405/139‑1 (MQ=255)
              cTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATc                                                                                             >  2:4347/1‑139 (MQ=255)
                                  ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCa                                                                         >  1:885342/1‑139 (MQ=255)
                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACAcc                                                                                                        >  2:613815/1‑105 (MQ=255)
                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACAcc                                                                                                        <  1:613815/105‑1 (MQ=255)
                                     cGTTGAGAACGCCGGTGGCCTGAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt                                                                                                                   <  2:790195/94‑1 (MQ=255)
                                     cGTTGAGAACGCCGGTGGCCTGAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt                                                                                                                   >  1:790195/1‑94 (MQ=255)
                                     cGTTGAGAACGCCGGTGACCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                            >  2:26313/1‑101 (MQ=255)
                                     cGTTGAGAACGCCGGTGACCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                            <  1:26313/101‑1 (MQ=255)
                                      gTTGAGAACGCCGGTGGCATGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTc                                                                     >  1:708198/1‑139 (MQ=255)
                                          agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttct                                                                                                                 >  1:848462/1‑90 (MQ=255)
                                          agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttct                                                                                                                 <  2:848462/92‑3 (MQ=255)
                                           gaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTAc                                                                                                >  2:712906/1‑107 (MQ=255)
                                           gaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTAc                                                                                                <  1:712906/107‑1 (MQ=255)
                                                ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcaca                                                                                                          >  2:4414/1‑92 (MQ=255)
                                                ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcaca                                                                                                          <  1:4414/92‑1 (MQ=255)
                                                      gCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTc                                                     >  2:280152/1‑139 (MQ=255)
                                                           aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACAcc                                                                                                        <  1:1058359/83‑1 (MQ=255)
                                                           aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACAcc                                                                                                        >  2:1058359/1‑83 (MQ=255)
                                                               aCATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaa                                            <  2:1004436/139‑1 (MQ=255)
                                                                         gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                           <  2:1048613/98‑1 (MQ=255)
                                                                         gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                           >  1:1048613/1‑98 (MQ=255)
                                                                             aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATa                                                                                       <  2:528265/82‑1 (MQ=255)
                                                                             aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATa                                                                                       >  1:528265/1‑82 (MQ=255)
                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                            >  1:582634/1‑48 (MQ=255)
                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                            <  2:582634/48‑1 (MQ=255)
                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaa                                            <  2:364557/112‑1 (MQ=255)
                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaa                                            >  1:364557/1‑112 (MQ=255)
                                                                                            aaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                           >  2:507273/1‑47 (MQ=255)
                                                                                            aaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                           <  1:507273/47‑1 (MQ=255)
                                                                                                         gAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc  <  2:437980/139‑1 (MQ=255)
                                                                                                              gcgcTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt               <  1:706682/121‑1 (MQ=255)
                                                                                                              gcgcTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt               >  2:706682/1‑121 (MQ=255)
                                                                                                                            |                                                                                                                        
AAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAC  >  NC_000913/1369912‑1370156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: