| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,624,356 | (T)8→6 | coding (141‑142/393 nt) | ydeI ← | hydrogen peroxide resistance OB fold protein, putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,624,350 | 0 | T | . | 97.5% | 158.3 / ‑1.9 | 40 | coding (148/393 nt) | ydeI | hydrogen peroxide resistance OB fold protein, putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (17/22); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
| * | NC_000913 | 1,624,351 | 0 | T | . | 90.0% | 128.9 / 11.4 | 40 | coding (147/393 nt) | ydeI | hydrogen peroxide resistance OB fold protein, putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base . (16/20); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACA > NC_000913/1624217‑1624480 || gCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCACGGTTTTTGCGAAATCAACGGTCAtttttt < 1:717586/139‑1 (MQ=255) aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat < 1:636141/139‑1 (MQ=255) aTTTCCCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGa < 1:1073792/139‑1 (MQ=255) tttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGAt < 1:699885/139‑1 (MQ=255) aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGcc > 2:538510/1‑139 (MQ=255) aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGcc > 1:44974/1‑139 (MQ=255) aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGcc < 1:545425/139‑1 (MQ=255) ctgCTATTAACGCGAAAAACGTAACGGTCCCGACAGTTGGGAGAAATGAGATTACCGCGTAGTGAAACCGAAGCGCCTTCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc < 2:941253/137‑1 (MQ=255) cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCAt < 2:51536/139‑1 (MQ=255) ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCAtct > 1:51901/1‑139 (MQ=255) ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCAtct < 1:538510/139‑1 (MQ=255) aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATg > 1:275549/1‑139 (MQ=255) gTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggcgg < 2:144839/139‑1 (MQ=255) gTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggcgg < 2:51901/139‑1 (MQ=255) ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcgc > 2:786173/1‑139 (MQ=255) tttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc < 2:673265/139‑1 (MQ=255) tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTc < 1:622486/91‑1 (MQ=255) tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGa > 1:489927/1‑96 (MQ=255) tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGa < 2:489927/96‑1 (MQ=255) tgagagaAATCGAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTCTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTc > 2:622486/4‑91 (MQ=255) aTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAg < 1:354796/91‑1 (MQ=255) aTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAg > 2:354796/1‑91 (MQ=255) cgcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTa < 2:958926/91‑1 (MQ=255) cgcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTa > 1:958926/1‑91 (MQ=255) gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc > 1:333165/1‑119 (MQ=255) gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc < 2:333165/119‑1 (MQ=255) gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc > 2:697074/1‑139 (MQ=255) aGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGAGAAATCAACGGTCATTTTTTTTGCTTCATCGCTTCTGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCAt > 2:359739/1‑139 (MQ=255) gAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc < 1:818108/139‑1 (MQ=255) gAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa < 1:786173/139‑1 (MQ=255) gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa > 2:881056/1‑139 (MQ=255) gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATaa > 1:334523/1‑139 (MQ=255) gcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAgg > 1:782707/1‑139 (MQ=255) ggTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaa > 2:866158/1‑139 (MQ=255) gTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCTGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGATCATCTGCCAACGCATAAGGCATGACAAGaaaa < 2:782707/139‑1 (MQ=255) gTTTTTGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa < 1:986644/139‑1 (MQ=255) tGCGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGc < 1:624040/139‑1 (MQ=255) cGAAATCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa < 2:44974/139‑1 (MQ=255) aaTCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATa > 2:890425/1‑139 (MQ=255) aTCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATAc > 2:45855/1‑139 (MQ=255) tCAACGGTCA‑‑TTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa < 2:883285/139‑1 (MQ=255) || GCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACA > NC_000913/1624217‑1624480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |