Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,200,454 (A)8→7 coding (176/3633 nt) yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,200,4470A.100.0% 155.4 / NA 37coding (169/3633 nt)yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/17);  total (20/17)

AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCG  >  NC_000913/2200309‑2200579
                                                                                                                                          |                                                                                                                                    
aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaaga                                                                                                                                      >  1:970500/1‑139 (MQ=255)
        aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                              <  2:442924/139‑3 (MQ=255)
                           aaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGaa                                                                                                          <  2:970500/139‑1 (MQ=255)
                              ggCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAAttt                                                                                                       >  2:841858/1‑139 (MQ=255)
                                 aaaaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTc                                                                                                    >  2:677820/1‑139 (MQ=255)
                                        cgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAg                                                                                             <  2:475768/139‑1 (MQ=255)
                                                           gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                          <  1:32244/139‑1 (MQ=255)
                                                                gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                     >  1:198150/1‑139 (MQ=255)
                                                                           tCCGTTATCACTGGAAGTCTCGTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGAt                                                          >  1:697165/1‑139 (MQ=255)
                                                                             cGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTGTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCg                                                        >  1:640119/1‑139 (MQ=255)
                                                                                tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGATAAGAAAAAAACATTGTCTGGCGTTATGAGGATTTTTCACTGGAGTGGCAGTCCGCCATCATGCAGGCCATCAACTTGATCGGCg                                                     >  2:707670/1‑139 (MQ=255)
                                                                                   cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                  >  2:882786/1‑139 (MQ=255)
                                                                                     cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAacac                                                >  1:33444/1‑139 (MQ=255)
                                                                                            tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                     >  1:1062049/1‑111 (MQ=255)
                                                                                            tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                     <  2:1062049/111‑1 (MQ=255)
                                                                                              tcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTAt                                                                                                               >  2:942066/1‑67 (MQ=255)
                                                                                              tcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTAt                                                                                                               <  1:942066/67‑1 (MQ=255)
                                                                                                 tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                                    <  1:882786/139‑1 (MQ=255)
                                                                                                        aTTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAGCATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                     >  1:655174/1‑83 (MQ=255)
                                                                                                        aTTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                     <  2:655174/83‑1 (MQ=255)
                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                     >  2:797429/1‑81 (MQ=255)
                                                                                                          tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                     <  1:797429/81‑1 (MQ=255)
                                                                                                            ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                         >  1:361290/1‑139 (MQ=255)
                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCa                                                                 <  2:808066/95‑1 (MQ=255)
                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCa                                                                 >  1:808066/1‑95 (MQ=255)
                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                     >  2:913424/1‑139 (MQ=255)
                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                     >  2:254402/1‑139 (MQ=255)
                                                                                                                atgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                     >  1:226033/1‑139 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               >  1:227707/1‑139 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  2:361290/139‑1 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  2:227707/139‑1 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:913424/139‑1 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:841858/139‑1 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:677820/139‑1 (MQ=255)
                                                                                                                      tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg               <  1:254402/139‑1 (MQ=255)
                                                                                                                        cTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCt             >  2:559654/1‑139 (MQ=255)
                                                                                                                            aaaaaGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                     <  2:20499/63‑1 (MQ=255)
                                                                                                                            aaaaaGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                     >  1:20499/1‑63 (MQ=255)
                                                                                                                                   tGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATcg  <  2:226033/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCG  >  NC_000913/2200309‑2200579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: