| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,765,829 | (C)5→4 | intergenic (‑53/‑89) | yfjM ← / → rnlA | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,765,825 | 0 | C | . | 98.1% | 212.6 / ‑3.0 | 52 | intergenic (‑49/‑93) | yfjM/rnlA | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (24/27); minor base G (0/1); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTC > NC_000913/2765687‑2765953 | cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGc < 2:797993/139‑1 (MQ=255) gAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTg > 1:566955/1‑139 (MQ=255) aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGc > 1:289134/1‑139 (MQ=255) aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCaa > 2:1049859/1‑139 (MQ=255) gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca < 1:511159/139‑1 (MQ=255) ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac > 1:729662/1‑139 (MQ=255) ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac > 1:214474/1‑139 (MQ=255) tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg < 1:547940/139‑1 (MQ=255) gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg < 2:551668/139‑1 (MQ=255) gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTCGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg < 2:609270/139‑1 (MQ=255) tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg < 1:136587/139‑1 (MQ=255) tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg > 1:107283/1‑139 (MQ=255) tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg > 2:362235/1‑139 (MQ=255) agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg < 1:362235/139‑1 (MQ=255) agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg < 2:566955/139‑1 (MQ=255) tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat < 2:107283/139‑1 (MQ=255) tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat < 1:889679/139‑1 (MQ=255) aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg > 1:320523/1‑111 (MQ=255) aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg < 2:320523/111‑1 (MQ=255) aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtatta > 2:740646/1‑139 (MQ=255) gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg < 1:590042/103‑1 (MQ=255) gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg > 2:590042/1‑103 (MQ=255) ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgg < 1:576539/121‑1 (MQ=255) ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgg > 2:576539/1‑121 (MQ=255) tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGtt < 2:729662/139‑1 (MQ=255) tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGtt > 1:48374/1‑139 (MQ=255) ccGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCa < 1:114366/84‑1 (MQ=255) ccGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCa > 2:114366/1‑84 (MQ=255) ccGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg < 1:135914/117‑1 (MQ=255) ccGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg > 2:135914/1‑117 (MQ=255) aaaCGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcccc < 1:1049859/139‑1 (MQ=255) aaaCGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcccc < 1:740646/139‑1 (MQ=255) aaCGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa < 2:214474/139‑1 (MQ=255) gccagccccaATTTTGT‑GAATT‑CCCCCCCCGTAGACGGTTCTGGCCTTTTATTGGAGGGGCCCTTA‑TCCGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt < 1:143312/134‑1 (MQ=255) tttGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGa > 2:434569/1‑139 (MQ=255) ttGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta < 1:368037/97‑1 (MQ=255) ttGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta > 2:368037/1‑97 (MQ=255) tgtgGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg > 2:749777/1‑71 (MQ=255) tgtgGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg < 1:749777/71‑1 (MQ=255) tgGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTACAAAATAAAAAACCTTAAGGGGGGTTTTTTGTTAGGGTTTCCCATTATGTTCCTATGGATTCAAGGatggt > 2:143312/1‑135 (MQ=255) tG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta < 2:368760/89‑1 (MQ=255) tG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta > 1:368760/1‑89 (MQ=255) cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCa > 2:877946/1‑64 (MQ=255) cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCa < 1:877946/64‑1 (MQ=255) cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAAt < 1:841009/67‑1 (MQ=255) cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAAt > 2:841009/1‑67 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcccc > 1:591996/1‑86 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcccc < 2:591996/86‑1 (MQ=255) gCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt < 2:289134/139‑1 (MQ=255) taGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTGTGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc < 1:188199/139‑1 (MQ=255) taGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTGTGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc > 2:188199/1‑139 (MQ=255) ttGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt > 2:897057/1‑108 (MQ=255) ttGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt < 1:897057/108‑1 (MQ=255) | CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTC > NC_000913/2765687‑2765953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |