Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	281,662	Δ1 bp	coding (322/1155 nt)	yagA ←	CP4‑6 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	281,660	0	G	.	100.0%	116.2 / NA	28	coding (324/1155 nt)	yagA	CP4‑6 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/14);  total (14/14)

GAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGA  >  NC_000913/281524‑281789                                                                                                                                         |                                                                                                                                  gAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggc                                                                                                                                 <  1:3247/139‑2 (MQ=255)  aaaGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggca                                                                                                                                <  2:801241/139‑3 (MQ=255)            ttAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGccc                                                                                                                     >  2:815004/1‑139 (MQ=255)                         gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGc                                                                                                        <  2:891052/139‑1 (MQ=255)                                  ggTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggca                                                                                                                                <  2:49099/107‑3 (MQ=255)                                  ggTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggca                                                                                                                                >  1:49099/1‑105 (MQ=255)                                   gTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTaa                                                                                              >  1:393544/1‑139 (MQ=255)                                    ttCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAAt                                                                                             >  2:1040130/1‑139 (MQ=255)                                        gcgcGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTg                                                                                         >  1:552936/1‑139 (MQ=255)                                           cgtcgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCgg                                                                                      <  2:599286/139‑1 (MQ=255)                                            gtcgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCggg                                                                                     <  1:1040130/139‑1 (MQ=255)                                                   tCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCa                                                                              <  1:815004/139‑1 (MQ=255)                                                            cccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATg                                                                     >  1:672428/1‑139 (MQ=255)                                                                 ggCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGt                                                                >  1:893678/1‑139 (MQ=255)                                                                                 gAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACgcag                                                <  1:430272/139‑1 (MQ=255)                                                                                     cgcCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAgg                                            >  2:838541/1‑139 (MQ=255)                                                                                                          gggCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                       <  2:924297/139‑1 (MQ=255)                                                                                                            gCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                       >  1:917437/1‑137 (MQ=255)                                                                                                            gCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                       <  2:917437/137‑1 (MQ=255)                                                                                                            gCCATCAGGTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCg                     <  1:17605/139‑1 (MQ=255)                                                                                                                   ggTTATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCggg              <  1:147731/139‑1 (MQ=255)                                                                                                                     ttATGGACGGTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGa            <  2:893678/139‑1 (MQ=255)                                                                                                                              gTGCTGAAGGC‑GGCTTGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                       <  1:689493/119‑1 (MQ=255)                                                                                                                              gTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                    >  2:163221/1‑74 (MQ=255)                                                                                                                              gTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                    <  1:163221/74‑1 (MQ=255)                                                                                                                              gTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                       <  1:845645/119‑1 (MQ=255)                                                                                                                              gTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                       >  2:845645/1‑119 (MQ=255)                                                                                                                              gTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACAGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg                       >  2:689493/1‑119 (MQ=255)                                                                                                                              gTGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCCCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCgg   >  1:486259/1‑139 (MQ=255)                                                                                                                               tGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGa            >  2:398203/1‑129 (MQ=255)                                                                                                                               tGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGa            <  1:398203/129‑1 (MQ=255)                                                                                                                               tGCTGAAGGC‑GGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGa  >  1:45499/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                  GAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGGA  >  NC_000913/281524‑281789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑