Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,159,054 Δ1 bp coding (565/918 nt) oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,159,0510G.98.0% 211.0 / ‑2.2 51coding (562/918 nt)oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (27/23);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCG  >  NC_000913/4158933‑4159185
                                                                                                                      |                                                                                                                                      
ggTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTgg                                                                                                                   <  2:164277/139‑1 (MQ=255)
ggTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTgg                                                                                                                   >  2:499645/1‑139 (MQ=255)
    aaaGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGa                                                                                                               <  1:157705/139‑1 (MQ=255)
      agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATg                                                                                                             >  2:334513/1‑139 (MQ=255)
          agCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCa                                                                                                         <  1:620629/139‑1 (MQ=255)
           gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGa                                                                                                               >  1:822536/1‑132 (MQ=255)
           gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGa                                                                                                               <  2:822536/132‑1 (MQ=255)
           gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCAc                                                                                                        >  1:1075492/1‑139 (MQ=255)
           gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCAc                                                                                                        >  1:154747/1‑139 (MQ=255)
                       ttGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCAAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                            <  2:679908/139‑1 (MQ=255)
                              gCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                     <  2:973225/139‑1 (MQ=255)
                               ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTgg                                                                                                                   <  1:978264/108‑1 (MQ=255)
                               ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTgg                                                                                                                   >  2:978264/1‑108 (MQ=255)
                                  ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATggg                                                                                 >  2:602884/1‑139 (MQ=255)
                                                      gCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                             <  1:602884/139‑1 (MQ=255)
                                                       cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAgggaaa                                                                                                                                   <  1:1030855/69‑4 (MQ=255)
                                                       cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGaaa                                                                                                                                   >  2:1030855/1‑69 (MQ=255)
                                                            tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                       >  2:868401/1‑139 (MQ=255)
                                                              tctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTg                                                                                                  >  2:530557/1‑94 (MQ=255)
                                                              tctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTg                                                                                                  <  1:530557/94‑1 (MQ=255)
                                                                     aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGcgcg                                                                                              <  2:514890/91‑1 (MQ=255)
                                                                     aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGcgcg                                                                                              >  1:514890/1‑91 (MQ=255)
                                                                      gATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacaca                                             >  2:143816/1‑139 (MQ=255)
                                                                       aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAActgc                                                                                                                            <  1:387738/59‑1 (MQ=255)
                                                                       aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAActgc                                                                                                                            >  2:387738/1‑59 (MQ=255)
                                                                        tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACt                                           >  1:560976/1‑139 (MQ=255)
                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAggg                                                                                                                                      >  1:819991/1‑48 (MQ=255)
                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAggg                                                                                                                                      <  2:819991/48‑1 (MQ=255)
                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACt                                           >  1:489327/1‑138 (MQ=255)
                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACt                                           <  2:489327/138‑1 (MQ=255)
                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                                                                                <  1:841198/84‑1 (MQ=255)
                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                                                                                >  2:841198/1‑84 (MQ=255)
                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                            <  1:32851/88‑1 (MQ=255)
                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                            >  2:32851/1‑88 (MQ=255)
                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATTGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                         >  1:740545/1‑139 (MQ=255)
                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                         >  1:398605/1‑139 (MQ=255)
                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGCCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                         >  1:852858/1‑139 (MQ=255)
                                                                            ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                       >  1:837460/1‑139 (MQ=255)
                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                             <  2:398680/107‑1 (MQ=255)
                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                             >  1:398680/1‑107 (MQ=255)
                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                             >  2:294686/1‑139 (MQ=255)
                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCAGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                             >  1:648984/1‑139 (MQ=255)
                                                                                               gCGTACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct                    <  2:978966/139‑1 (MQ=255)
                                                                                                  tACCGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                 <  2:398605/139‑1 (MQ=255)
                                                                                                    ccGATGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg               <  2:837460/139‑1 (MQ=255)
                                                                                                       aTGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                >  2:87826/1‑87 (MQ=255)
                                                                                                       aTGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                <  1:87826/87‑1 (MQ=255)
                                                                                                        tGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                              >  1:1005138/1‑88 (MQ=255)
                                                                                                        tGGCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                              <  2:1005138/88‑1 (MQ=255)
                                                                                                         ggCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTgg                         >  2:541403/1‑124 (MQ=255)
                                                                                                         ggCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTgg                         <  1:541403/124‑1 (MQ=255)
                                                                                                         ggCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACa          >  2:598239/1‑139 (MQ=255)
                                                                                                         ggCCGATCTGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACa          <  1:334513/139‑1 (MQ=255)
                                                                                                                 tGGCAGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcg  <  2:154747/139‑1 (MQ=255)
                                                                                                                      |                                                                                                                                      
GGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCG  >  NC_000913/4158933‑4159185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: