Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 401,548 (G)5→4 coding (163/261 nt) iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913401,5440G.100.0% 128.1 / NA 31coding (159/261 nt)iraPanti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/13);  total (18/13)

GTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAA  >  NC_000913/401409‑401676
                                                                                                                                       |                                                                                                                                    
gttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgggg                                                                                                                                   <  2:517997/139‑1 (MQ=255)
  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  1:124471/139‑3 (MQ=255)
  tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCACAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  2:989994/139‑3 (MQ=255)
      tAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGt                                                                                                                            >  1:644167/1‑139 (MQ=255)
        aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                          >  2:880283/1‑139 (MQ=255)
        aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                          <  1:880283/139‑1 (MQ=255)
         gCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTCGATTGATCAGGTAGAGGGGCGCTGTACg                                                                                                                         <  1:664905/139‑1 (MQ=255)
           ttGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAg                                                                                                                       >  2:629275/1‑139 (MQ=255)
              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTg                                                                                                                             <  2:831136/130‑1 (MQ=255)
              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTg                                                                                                                             >  1:831136/1‑130 (MQ=255)
              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTa                                                                                                                    >  1:809442/1‑139 (MQ=255)
              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTa                                                                                                                    <  2:261417/139‑1 (MQ=255)
              cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCACAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTa                                                                                                                    >  2:807236/1‑139 (MQ=255)
                                     gAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTga                                                                                             <  2:809442/139‑1 (MQ=255)
                                                gcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                          >  2:531318/1‑99 (MQ=255)
                                                gcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                          <  1:531318/99‑1 (MQ=255)
                                                                     tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTc                                                                                                 >  1:134757/1‑103 (MQ=255)
                                                                     tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTc                                                                                                 <  2:134757/103‑1 (MQ=255)
                                                                      aTCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa                                                            <  2:644167/139‑1 (MQ=255)
                                                                        cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                          >  2:421206/1‑139 (MQ=255)
                                                                              tGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTAt                                                    >  1:28217/1‑139 (MQ=255)
                                                                              tGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTAt                                                    >  1:603632/1‑139 (MQ=255)
                                                                                  aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                <  2:28217/139‑1 (MQ=255)
                                                                                  aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                <  1:421206/139‑1 (MQ=255)
                                                                                     cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCAAGGCCAGCTTTCCTGCGGAGGTTAGGGGGCTGTTGGGGGATTACGAAAAAAGGTAATTAAAgat                                             >  1:996872/1‑137 (MQ=255)
                                                                                        cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 >  1:821355/1‑51 (MQ=255)
                                                                                        cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  2:821355/53‑3 (MQ=255)
                                                                                        cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                          >  1:437300/1‑139 (MQ=255)
                                                                                          cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                           >  2:473299/1‑120 (MQ=255)
                                                                                          cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                           <  1:473299/120‑1 (MQ=255)
                                                                                            atatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGt                                      <  1:629275/139‑1 (MQ=255)
                                                                                                    cAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                          <  1:753010/47‑1 (MQ=39)
                                                                                                    cAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGCGCTGTAc                                                                                                                          >  2:753010/1‑47 (MQ=39)
                                                                                                                          ttgatCAGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGt        >  1:615206/1‑139 (MQ=255)
                                                                                                                                aGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaaa  >  2:387278/1‑139 (MQ=255)
                                                                                                                                aGGTAGAGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGACATaaa  >  1:894279/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAA  >  NC_000913/401409‑401676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: