Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,002,224	(G)7→8	coding (418/1071 nt)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,217	1	.	G	100.0%	93.6 / NA	27	T137T (ACA→ACG)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (12/15);  total (12/15)

AGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCAT  >  NC_000913/1002106‑1002342                                                                                                                 |                                                                                                                              aGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTg                                                                                                     <  1:958318/139‑1 (MQ=255)   tCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTg                                                                                                     >  2:660308/1‑137 (MQ=255)   tCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTg                                                                                                     <  1:660308/137‑1 (MQ=255)            gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCg                                                                                          >  2:967422/1‑139 (MQ=255)             cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑ggg                                                                                                                            >  2:188256/1‑103 (MQ=255)             cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑ggg                                                                                                                            <  1:188256/103‑1 (MQ=255)              gACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                        <  2:1038995/139‑1 (MQ=255)              gACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                                        <  1:338269/139‑1 (MQ=255)                   cTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACg                                                                                   <  2:385245/139‑1 (MQ=255)                           aaaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTg                                                                           <  1:107206/139‑1 (MQ=255)                                aaTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGc                                                                                           >  1:456271/1‑118 (MQ=255)                                aaTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGc                                                                                           <  2:456271/118‑1 (MQ=255)                                    aCTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggggg                                                                  <  1:62281/139‑1 (MQ=255)                                        tCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACACTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAg                                                              >  1:689442/1‑139 (MQ=255)                                         cTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGc                                                             <  1:834562/139‑1 (MQ=255)                                          ttGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCg                                                            <  1:967422/139‑1 (MQ=255)                                                                ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCTATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                                      <  2:689442/139‑1 (MQ=255)                                                                ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                                >  1:793360/1‑113 (MQ=255)                                                                ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                                <  2:793360/113‑1 (MQ=255)                                                                ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                                      >  2:552574/1‑139 (MQ=255)                                                                      aGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAat                                >  2:612064/1‑139 (MQ=255)                                                                           gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGc                           >  1:933105/1‑139 (MQ=255)                                                                            ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCa                          >  1:158060/1‑139 (MQ=255)                                                                                         gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGttt                                                                                <  1:388621/72‑1 (MQ=255)                                                                                         gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGttt                                                                                >  2:388621/1‑72 (MQ=255)                                                                                         gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc             >  1:54319/1‑139 (MQ=255)                                                                                            ccTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCg          >  2:481534/1‑139 (MQ=255)                                                                                                  gAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcgtc    <  2:933105/139‑1 (MQ=255)                                                                                                    aaaCGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCAt  <  1:612064/139‑1 (MQ=255)                                                                                                                 |                                                                                                                              AGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCAT  >  NC_000913/1002106‑1002342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑