| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,920,205 | (T)7→8 | intergenic (+62/‑18) | yebT → / → rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,920,198 | 1 | . | T | 96.3% | 81.6 / ‑3.9 | 27 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (15/11); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC > NC_000913/1920064‑1920328 | cccAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑tttt > 1:491109/1‑139 (MQ=255) ggAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑tttttttt < 1:32356/139‑2 (MQ=255) aaGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑ttttttttgc < 2:491109/139‑4 (MQ=255) aaGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑ttttttttgc < 1:401188/139‑4 (MQ=255) ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGc > 1:810370/1‑139 (MQ=255) tGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTg < 1:902184/139‑1 (MQ=255) aGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGcc < 1:577348/139‑1 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGcc > 1:594987/1‑102 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGcc < 2:594987/102‑1 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacac < 1:257999/108‑1 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacac > 2:257999/1‑108 (MQ=255) aatGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCg < 2:668174/139‑1 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg > 1:605758/1‑139 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑ttttttttgcca < 1:414477/66‑6 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑ttttttttgcca > 2:414477/1‑61 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑ttttttttgc > 2:979748/1‑61 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑ttttttttgc < 1:979748/64‑4 (MQ=255) aCTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCtt > 2:209655/1‑139 (MQ=255) cTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAg < 1:673782/62‑1 (MQ=255) cTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAg > 2:673782/1‑62 (MQ=255) tCCGGCGTGCCTGCGGCGGGGCGTTTATGCTAAACTGCGGGCCTGG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACg < 2:271536/139‑1 (MQ=255) cGGCGTGCCTGCGCGGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAg > 1:652067/1‑56 (MQ=255) cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAg < 2:652067/56‑1 (MQ=255) cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCg < 2:1039413/69‑1 (MQ=255) cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCg > 1:1039413/1‑69 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAg > 2:63503/1‑51 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAg < 1:63503/51‑1 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACa < 2:983942/58‑1 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACa > 1:983942/1‑58 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTTTGACCCAAATCCGCGAAGCGATGCCTTCGACGCCCCCAttt > 1:271536/1‑139 (MQ=255) ccGGAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTgatgat > 1:992644/1‑139 (MQ=255) gAGCGTTTATGCTAAACTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGAtttt > 1:227958/1‑139 (MQ=255) aaaCTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTc > 2:332363/1‑139 (MQ=255) cTGCGCGCCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAgc < 1:209655/139‑1 (MQ=255) cgcgcCT‑GTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 1:157834/1‑139 (MQ=255) | CCCAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCT‑G‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC > NC_000913/1920064‑1920328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |