Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,484,897 (C)6→7 coding (524/3594 nt) evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,484,8911.C98.4% 223.2 / ‑3.9 64coding (518/3594 nt)evgShybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (31/32);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAC  >  NC_000913/2484754‑2485020
                                                                                                                                           |                                                                                                                                 
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                  >  1:935636/1‑139 (MQ=255)
ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                  <  2:617821/139‑1 (MQ=255)
   gccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCCCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccc                                                                                                                               >  2:793838/1‑139 (MQ=255)
     cgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccc                                                                                                                             >  2:962220/1‑139 (MQ=255)
         tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                         <  1:962220/139‑5 (MQ=255)
         tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                         <  1:178108/139‑5 (MQ=255)
           ttAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACg                                                                                                                        <  1:179216/139‑1 (MQ=255)
            taataaTGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGa                                                                                                                       >  2:513827/1‑139 (MQ=255)
                aatGACATTGCTGCAACCAACCCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTa                                                                                                                   <  1:820228/139‑1 (MQ=255)
                  tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                         >  2:866882/1‑126 (MQ=255)
                  tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                         <  1:866882/130‑5 (MQ=255)
                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATtc                                                                                                               >  1:267214/1‑139 (MQ=255)
                       ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                              <  1:943813/120‑1 (MQ=255)
                       ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                              >  2:943813/1‑120 (MQ=255)
                          ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                  >  1:651546/1‑113 (MQ=255)
                          ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACCCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                  <  2:651546/113‑1 (MQ=255)
                           tgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCatcaatc                                                                                                        >  2:136467/1‑139 (MQ=255)
                               accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCAttt                                                                                                    >  2:511918/1‑139 (MQ=255)
                                    accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                               >  1:629063/1‑139 (MQ=255)
                                       aTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                            <  1:454000/139‑1 (MQ=255)
                                        ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGca                                                                                           >  2:308544/1‑139 (MQ=255)
                                        ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGca                                                                                           <  2:1013395/139‑1 (MQ=255)
                                              aTTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt                                                                                     <  2:199415/139‑1 (MQ=255)
                                               ttACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa                                                                                    >  2:300778/1‑139 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTa                                                                                                                   <  1:1034898/107‑1 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTa                                                                                                                   >  2:1034898/1‑107 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                   <  1:511918/139‑1 (MQ=255)
                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                   >  1:330319/1‑139 (MQ=255)
                                                 aCCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc                                                                                  <  1:513827/139‑1 (MQ=255)
                                                     ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTCCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGGCGAGGTAATTCATCAATAATTTCCAAAAGCAACAATTATCTCtt                                                                              >  2:980382/1‑139 (MQ=255)
                                                      tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt                                                                             >  2:746364/1‑139 (MQ=255)
                                                                   accaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                <  2:935636/139‑1 (MQ=255)
                                                                    ccaccaTTCACGACTCAATGCGACCTCTTACCTCACCAAAACCAGTAAACATTGCTAGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTATTTTACAAATTTAGATc                                                               <  2:800928/139‑1 (MQ=255)
                                                                    ccaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                               <  1:746364/139‑1 (MQ=255)
                                                                        ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt                                                                      <  1:41394/128‑1 (MQ=255)
                                                                        ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt                                                                      >  2:41394/1‑128 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                         >  2:815456/1‑139 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                         >  2:126208/1‑139 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                              <  1:112383/69‑1 (MQ=255)
                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                              >  2:112383/1‑69 (MQ=255)
                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                    >  2:28531/1‑139 (MQ=255)
                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                    <  1:28531/139‑1 (MQ=255)
                                                                                 cTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc                                                  >  1:168438/1‑139 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                    >  1:976584/1‑136 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                    <  2:976584/136‑1 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                 >  1:478211/1‑139 (MQ=255)
                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                 >  1:8450/1‑139 (MQ=255)
                                                                                    aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt                                               <  2:330319/139‑1 (MQ=255)
                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                >  2:556018/1‑120 (MQ=255)
                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                <  1:556018/120‑1 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACg                                                                                                                        >  1:282177/1‑59 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACg                                                                                                                        <  2:282177/59‑1 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGt                                                                                                                    >  1:692169/1‑63 (MQ=255)
                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGt                                                                                                                    <  2:692169/63‑1 (MQ=255)
                                                                                              cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                         >  1:653592/1‑50 (MQ=255)
                                                                                              cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                         <  2:653592/54‑5 (MQ=255)
                                                                                               ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                    <  2:267214/139‑1 (MQ=255)
                                                                                                tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                  <  2:770272/43‑1 (MQ=255)
                                                                                                tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                  >  1:770272/1‑43 (MQ=255)
                                                                                                      aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                             >  1:848112/1‑139 (MQ=255)
                                                                                                       ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                            <  1:300778/139‑1 (MQ=255)
                                                                                                        cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAtt                           <  2:848112/139‑1 (MQ=255)
                                                                                                           aaCCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTcc                                                                                                  >  2:35313/1‑65 (MQ=255)
                                                                                                           aaCCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTcc                                                                                                  <  1:35313/65‑1 (MQ=255)
                                                                                                                gTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                           >  1:744578/1‑83 (MQ=255)
                                                                                                                gTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                           <  2:744578/83‑1 (MQ=255)
                                                                                                                gTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt                   <  2:478211/139‑1 (MQ=255)
                                                                                                                 taaatagtacaagggTAGCAAATGTCCACCCCAGACTGGGTAATTCATCAATCATTCCCAAAAGCAAAATTTATCACTATTACAGATTTATCTCAGGCATTAGCATCCGTATCAGGTGGGCACAATGATTCCGTTATTg                  <  2:323498/127‑1 (MQ=255)
                                                                                                                 tAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTg                  <  2:8450/139‑1 (MQ=255)
                                                                                                                  aaaTATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                           >  1:257788/1‑81 (MQ=255)
                                                                                                                  aaaTATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                           <  2:257788/81‑1 (MQ=255)
                                                                                                                  aaaTATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg                 <  1:1034016/139‑1 (MQ=255)
                                                                                                                        tGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa           <  1:126208/139‑1 (MQ=255)
                                                                                                                         gCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                 <  2:740803/52‑1 (MQ=255)
                                                                                                                         gCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                 >  1:740803/1‑52 (MQ=255)
                                                                                                                         gCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACt                        <  2:587579/125‑1 (MQ=255)
                                                                                                                         gCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACt                        >  1:587579/1‑125 (MQ=255)
                                                                                                                           tCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat        >  1:542211/1‑139 (MQ=255)
                                                                                                                               gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                 <  2:4921/46‑1 (MQ=255)
                                                                                                                               gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                 >  1:4921/1‑46 (MQ=255)
                                                                                                                               gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCAtt    >  2:602986/1‑139 (MQ=255)
                                                                                                                                 aGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc  <  1:815456/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                 
TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAC  >  NC_000913/2484754‑2485020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: