Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,4381.C90.9% 96.2 / 2.4 33intergenic (+83/‑272)rnlB/yfjPCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base C (13/17);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGT  >  NC_000913/2767303‑2767572
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ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcct                                                                                                                                       >  2:813949/1‑138 (MQ=255)
ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccc                                                                                                                                       <  1:897594/139‑1 (MQ=255)
  tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccc                                                                                                                                    >  1:710743/1‑139 (MQ=255)
    tGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcaccacc                                                                                                                                  >  1:899724/1‑139 (MQ=255)
    tGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAacccccc                                                                                                                                  >  1:108772/1‑139 (MQ=255)
          gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                            <  2:710743/139‑1 (MQ=255)
               ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                       <  2:838477/139‑1 (MQ=255)
                  atgatgTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCAACCCTACAAAAAGTCCTg                                                                                                                    >  1:950627/1‑139 (MQ=255)
                               aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGATTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAggg                                                                                                             >  1:190592/1‑133 (MQ=255)
                               aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAggg                                                                                                             <  2:190592/133‑1 (MQ=255)
                                                             tttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTc                                                                         <  2:899724/139‑1 (MQ=255)
                                                               gtataagagacaggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc                                                                       <  1:50575/126‑1 (MQ=255)
                                                                                       gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                            >  2:825944/1‑62 (MQ=255)
                                                                                       gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                            <  1:825944/62‑1 (MQ=255)
                                                                                       gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                        >  2:818578/1‑66 (MQ=255)
                                                                                       gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                        <  1:818578/66‑1 (MQ=255)
                                                                                       gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                       <  1:848730/67‑1 (MQ=255)
                                                                                       gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                       >  2:848730/1‑67 (MQ=255)
                                                                                       gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCAAAACGTCCTGCATAGGGAACACGTTTTTCCATGTGAACCCAAGACAAAATTTACCCCACTTTAAAcctgtatcttataa                                               >  2:50575/1‑126 (MQ=255)
                                                                                                     atatTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                                 <  2:950627/139‑1 (MQ=255)
                                                                                                         tAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAAt                             <  1:813949/139‑1 (MQ=255)
                                                                                                                tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTACTTTTCCCCCCCTTTAAACTTCTCAATAATGTCAcc                                           >  2:603093/1‑118 (MQ=255)
                                                                                                                tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCAcc                                           <  1:603093/118‑1 (MQ=255)
                                                                                                                tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatga                      >  2:177233/1‑139 (MQ=255)
                                                                                                                tCCAATATGATCAAATAGCATTAACCCCACCATCACAACGTCCTGCATATTGAACACGTATTCCCCTGTGCACCCACGACTAAACTTCCCCCCCTTTAAACTTCTCAACAATGTAACCAATTTTCTGGAAATCCTataa                      >  1:62906/1‑137 (MQ=255)
                                                                                                                         aTCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCCCCCACGACTAAATTTccc                                                                       >  2:935898/1‑81 (MQ=255)
                                                                                                                         aTCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc                                                                       <  1:935898/81‑1 (MQ=255)
                                                                                                                          tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTcccc                                                                                               >  2:704984/1‑56 (MQ=255)
                                                                                                                          tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTcccc                                                                                               <  1:704984/56‑1 (MQ=255)
                                                                                                                          tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                    <  1:92045/131‑1 (MQ=255)
                                                                                                                          tCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTCTTCACCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                    >  2:92045/1‑131 (MQ=255)
                                                                                                                              aTAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTa        >  2:586168/1‑139 (MQ=255)
                                                                                                                              aTAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGACACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTa        >  2:338357/1‑139 (MQ=255)
                                                                                                                                aGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa      <  1:586168/139‑1 (MQ=255)
                                                                                                                                aGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa      <  1:338357/139‑1 (MQ=255)
                                                                                                                                   aTTAACCCCCCCCTCACAACGTCCTGGATAGGCAACACGTTTTCCCCTGTGCAcc                                                                                       >  2:510937/1‑55 (MQ=255)
                                                                                                                                   aTTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                       <  1:510937/55‑1 (MQ=255)
                                                                                                                                    ttAACCCCACCCTCAAAACGTCCTGCATAGGGAACACGATTTCCACAGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTACCAAAAAATTCAACCAATTTTAAGCAAACCGTATGAGACATTTCCTGGTAAATgga  >  2:531426/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGT  >  NC_000913/2767303‑2767572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: