| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,736,160 | +A | intergenic (+3/+193) | xylR → / ← bax | xylose divergent operon transcriptional activator/putative glucosaminidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,736,159 | 1 | . | A | 98.3% | 187.3 / ‑4.3 | 59 | intergenic (+2/+194) | xylR/bax | xylose divergent operon transcriptional activator/putative glucosaminidase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (29/29); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCG‑A‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAA > NC_000913/3736022‑3736287 | aaCCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACg‑a > 1:3371/1‑139 (MQ=255)aaCCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACg‑a > 2:224798/1‑139 (MQ=255) cTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAa‑tgatg > 1:605902/1‑139 (MQ=255) cTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAa‑tgatg > 2:691831/1‑139 (MQ=255) ttGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑tgatga > 1:564708/1‑139 (MQ=255) tCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGt < 1:642869/139‑1 (MQ=255) gagaTATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCg < 1:224798/139‑1 (MQ=255) atCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTcaca > 2:908450/1‑139 (MQ=255) atCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTcaca > 2:842100/1‑139 (MQ=255) atCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTcaca > 1:371246/1‑139 (MQ=255) atCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTcaca < 2:257485/139‑1 (MQ=255) cGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATc > 2:231155/1‑139 (MQ=255) tgttCGGTTATCAATCGCTGCAATATATCAACTCTGTTATTAAAAAAGCATATGACACGACGACAAAAGAGCATCGAGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAAAAGTGTCGGCCTCAAATCAGGTaa < 2:482152/139‑1 (MQ=255) ttATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg < 2:391307/139‑1 (MQ=255) ttATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg < 1:433496/139‑1 (MQ=255) ttATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg < 1:231155/139‑1 (MQ=255) tATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGt < 2:3371/139‑1 (MQ=255) tCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTgg > 2:20638/1‑139 (MQ=255) cTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTcaca > 2:814999/1‑114 (MQ=255) cTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTcaca < 1:814999/114‑1 (MQ=255) cTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTGACGCTGCGTGGTTGTCgg > 2:985597/1‑139 (MQ=255) cAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATg > 1:530637/1‑139 (MQ=255) tCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTGACGCTGCGTGGTTGTCGGATGCGGCGTg < 1:985597/139‑1 (MQ=255) cTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGa > 1:539236/1‑139 (MQ=255) cTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACG‑AGTGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGa > 2:562009/1‑139 (MQ=255) ctctGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACg < 2:787207/139‑1 (MQ=255) tctGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGc < 1:691831/139‑1 (MQ=255) tGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCt > 1:196037/1‑139 (MQ=255) tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTc < 1:262567/86‑1 (MQ=255) tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTc > 2:262567/1‑86 (MQ=255) tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTa > 1:357686/1‑139 (MQ=255) aaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa < 1:20638/139‑1 (MQ=255) aaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa < 1:842100/139‑1 (MQ=255) aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATgcg < 1:684766/115‑1 (MQ=255) aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATgcg > 2:684766/1‑115 (MQ=255) atGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGcc < 2:290252/139‑1 (MQ=255) cgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACg‑aatgat > 1:294804/1‑47 (MQ=255) cgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACg‑aatgat < 2:294804/52‑6 (MQ=255) cgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGcc > 1:694128/1‑130 (MQ=255) cgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGcc < 2:694128/130‑1 (MQ=255) gCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTgg > 1:261944/1‑88 (MQ=255) gCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTgg < 2:261944/88‑1 (MQ=255) ccAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaa < 2:885249/139‑1 (MQ=255) ccAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaa < 1:562009/139‑1 (MQ=255) cAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaaa > 2:963247/1‑139 (MQ=255) aaaaGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaaaa < 1:588452/139‑1 (MQ=255) gTATCGCGATGTAAATAGCGAGGTCATGTTGTGACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAcc > 2:639614/1‑139 (MQ=255) gcgATGTACATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTATCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaacaa < 1:639614/139‑1 (MQ=39) aTGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATgcg > 2:956501/1‑83 (MQ=255) aTGTAAATAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATgcg < 1:956501/83‑1 (MQ=255) aTAGCGAGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAgg > 1:559395/1‑139 (MQ=255) aGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaacaa > 1:861974/1‑124 (MQ=255) aGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaacaa < 2:861974/124‑1 (MQ=255) aGGTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTa > 1:391039/1‑139 (MQ=255) ggTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaacaa < 1:558320/123‑1 (MQ=255) ggTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaacaa > 2:558320/1‑123 (MQ=255) ggTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAg > 2:442745/1‑139 (MQ=255) ggTCATGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAg > 2:107389/1‑139 (MQ=255) aTGTTGTAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCa > 1:383447/1‑139 (MQ=255) tgttgtAACGAA‑TGATGAGAATTGTCGGCGTcaca > 1:751426/1‑35 (MQ=255) tgttgtAACGAA‑TGATGAGAATTGTCGGCGTcaca < 2:751426/35‑1 (MQ=255) tgttgtAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCaa < 2:605902/139‑1 (MQ=255) tgttgtAACGAA‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCaa < 2:539236/139‑1 (MQ=255) | AACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCG‑A‑TGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAA > NC_000913/3736022‑3736287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |