| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,970,032 | (G)6→7 | coding (1002/1047 nt) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,970,026 | 1 | . | G | 96.9% | 108.3 / ‑3.2 | 32 | coding (996/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (19/12); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGT > NC_000913/3969888‑3970156 | ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc > 2:829781/1‑139 (MQ=255) cacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGAt < 1:829781/139‑1 (MQ=255) cTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCggg < 1:269087/139‑1 (MQ=255) cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCAt > 1:985486/1‑139 (MQ=255) aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTa > 2:950544/1‑139 (MQ=255) aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg < 1:1034321/110‑1 (MQ=255) aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg > 2:1034321/1‑110 (MQ=255) tGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTT‑gggg‑ggg < 1:625213/111‑2 (MQ=255) tGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTT‑gggg‑ggg > 2:625213/1‑110 (MQ=255) gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAcc > 2:9038/1‑136 (MQ=255) gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAcc < 1:9038/136‑1 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 1:677444/1‑139 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg > 1:596432/1‑98 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg < 2:596432/98‑1 (MQ=255) ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:950544/139‑1 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac > 1:347785/1‑139 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac > 2:818390/1‑139 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑GGGGTGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAcca < 2:622192/139‑1 (MQ=255) ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtgctgc > 1:1069992/1‑139 (MQ=255) ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggg < 1:61939/59‑1 (MQ=255) ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggg > 2:61939/1‑59 (MQ=255) aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGTTCGCCGCt < 2:347785/139‑1 (MQ=255) aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGgt > 1:813607/1‑66 (MQ=255) aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGgt < 2:813607/66‑1 (MQ=255) gtataagagacagtgatTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTGTTCGAAGAGAATCGa < 2:717768/126‑1 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg > 1:1001059/1‑139 (MQ=255) gCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt > 1:840274/1‑139 (MQ=255) gatgatTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac < 2:677444/139‑1 (MQ=255) gatTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 2:985486/139‑1 (MQ=255) tATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg > 2:402956/1‑139 (MQ=255) tgcggggCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt > 2:432619/4‑58 (MQ=255) tgcggggCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:432619/55‑1 (MQ=255) tgcggggCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg > 2:405719/4‑109 (MQ=255) tgcggggCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg < 1:405719/106‑1 (MQ=255) tgcggggCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa > 1:985932/4‑139 (MQ=255) tgcggggCATTGTCGGGGG‑GGCTGATCGGGGCAGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa > 2:639398/4‑139 (MQ=255) ggCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTgg > 2:735872/1‑139 (MQ=255) ggCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCACTAACCTGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTCATTCGAAGAGAATCGATGTGAAAGTacctgtctcttatac > 1:717768/1‑126 (MQ=255) gCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa < 1:872585/105‑1 (MQ=255) gCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa > 2:872585/1‑105 (MQ=255) gCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGt > 1:912283/1‑139 (MQ=255) | GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGT > NC_000913/3969888‑3970156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |