Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,124	IS5 (+) +4 bp	intergenic (‑202/‑400)	hns ← / → tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1293124 =	0 (0.000)	64 (1.380)	37/216	0.2	100%	intergenic (‑202/‑403)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
	?	NC_000913	= 1300693	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1293127	0 (0.000)	60 (1.290)	43/216	0.1	100%	intergenic (‑205/‑400)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1292999‑1293127 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA  <  NC_000913/2290113‑2289979                                                                                                                                                                                                                                                                          AGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCG                                                                                                                               >  2:945657/1‑139       GTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAG                                                                                                                         <  2:333786/139‑1       GTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTAAGGTTAAGGAAGGTGCGAACAAG                                                                                                                         <  2:800662/139‑1        TAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGTATATAGCCCTATTTACACCGATGATTTCGCAACACGTGAGGTTAAGGAAGGTGCGAACAA                                                                                                                          >  2:770013/1‑137        TAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGT                                                                                                                        >  1:379882/1‑139        TAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAACACGTGAGGTTAAGGAAGGTGCGAACAA                                                                                                                          <  1:770013/137‑1        TAAGGAATTCAAAATTGTTC‑TTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTC                                                                                                                       <  2:510367/139‑1        TAAGGAATTCAAAATTGTTC‑TTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTC                                                                                                                       >  1:510367/1‑139          AGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCC                                                                                                                      <  1:626295/139‑1           GGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCC                                                                                                                     <  2:226013/139‑1                TCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATA                                                                                                                <  2:978576/139‑1                     ATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGA                                                                                                           <  1:886590/139‑1                      TTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGAT                                                                                                          <  2:379882/139‑1                         TTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCA                                                                                                        >  2:12025/1‑138                         TTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCA                                                                                                        <  1:12025/138‑1                               ATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGT                                                                                                 <  1:211158/139‑1                                     TGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAG                                                                                                                         <  2:749919/109‑1                                     TGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAG                                                                                                                         >  1:749919/1‑109                                       CCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCT                                                                                            >  1:452247/1‑136                                       CCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCT                                                                                            <  2:452247/136‑1                                        CGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGG                                                                                          <  2:132144/137‑1                                        CGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGG                                                                                          >  1:132144/1‑137                                        CGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGA                                                                                                                                      >  1:548602/1‑93                                        CGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGA                                                                                                                                      <  2:548602/93‑1                                          CCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGG                                                                                                                                       >  2:371021/1‑90                                          CCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGG                                                                                                                                       <  1:371021/90‑1                                                   ATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAG                                                                             <  1:945657/139‑1                                                     CTTTTCATAAAATTAGCCAGAAAAGACTCCGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCC                                                                                                                     <  2:626930/97‑1                                                     CTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCC                                                                                                                     >  1:626930/1‑97                                                      TTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGT                                                                          >  1:675762/1‑139                                                        TTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTC                                                                        >  1:132766/1‑139                                                          CATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAA                                                                                                                             <  1:105533/84‑1                                                          CATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAA                                                                                                                             >  2:105533/1‑84                                                               AATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGA                                                                 <  2:132766/139‑1                                                                         AAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCC                                                                                                                      <  2:572180/76‑1                                                                         AAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCC                                                                                                                      >  1:572180/1‑76                                                                               CGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTT                                                 >  2:410890/1‑139                                                                                GCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGA                                                                                                             >  2:202668/1‑78                                                                                GCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGA                                                                                                             <  1:202668/78‑1                                                                                        TAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCC                                                                                                                      >  2:372586/1‑61                                                                                        TAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCC                                                                                                                      <  1:372586/61‑1                                                                                              TATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCAc                                                            >  2:684953/1‑112                                                                                              TATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATT                                  >  1:432210/1‑139                                                                                              TATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCAT                                                            <  1:684953/113‑1                                                                                              TATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGG                                                                           >  1:566525/1‑98                                                                                              TATTTACACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGG                                                                           <  2:566525/98‑1                                                                                                     ACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCC                                                                                                                      >  1:1074469/1‑48                                                                                                     ACCGATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCC                                                                                                                      <  2:1074469/48‑1                                                                                                        GATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAG                        >  2:35269/1‑139                                                                                                        GATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                     >  2:13340/1‑78                                                                                                        GATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                     <  1:13340/78‑1                                                                                                         ATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAA                                                                                <  2:811/82‑1                                                                                                         ATGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAA                                                                                >  1:811/1‑82                                                                                                          TGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAA                                                                                <  2:617945/81‑1                                                                                                          TGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAA                                                                                >  1:617945/1‑81                                                                                                          TGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCC                                                                                                                     <  2:809707/44‑1                                                                                                          TGATTTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCC                                                                                                                     >  1:809707/1‑44                                                                                                              TTCGCAGCACGTGAGGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGT                  <  1:35269/139‑1                                                                                                                              TTAAGGTAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA  <  1:192495/139‑1                                                                                                                              TTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA  >  1:276618/1‑139                                                                                                                                                                                                                                                                          AGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1292999‑1293127 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA  <  NC_000913/2290113‑2289979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.