| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,287,219 | 0 | G | A | 35.7% | 27.7 / 17.6 | 28 | noncoding (18/171 nt) | rttR | rtT sRNA, processed from tyrT transcript |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base A (4/6); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAT > NC_000913/1287086‑1287343 | cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGtt > 2:832667/1‑139 (MQ=11) gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACtttt > 2:427213/1‑139 (MQ=255) gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGa < 1:427213/139‑1 (MQ=11) ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGaaa > 2:447272/1‑139 (MQ=11) cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGa < 1:832667/139‑1 (MQ=255) cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGa < 1:996973/139‑1 (MQ=255) ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggt < 1:671698/139‑1 (MQ=255) cGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtgg > 1:104672/1‑126 (MQ=11) cGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtgg < 2:104672/126‑1 (MQ=255) tCGAGCCGAAACGTGATCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAATATTTGATTGCGGATTCG‑CTAGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg < 1:576209/139‑1 (MQ=14) aCCTTAATCGAAGCTTCTCATCCTTCCCCGAATGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc < 2:901839/139‑1 (MQ=14) gCATGGGCAGTATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACTGTCTGCTCCCTTTGGCCGCTCGGGAAc < 1:695888/138‑1 (MQ=18) gCATGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcc < 2:1046083/139‑1 (MQ=21) gCATGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTGTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc > 2:695888/1‑138 (MQ=18) gggCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg > 1:203075/1‑112 (MQ=17) gggCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg < 2:203075/112‑1 (MQ=17) ggCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg < 2:100285/66‑1 (MQ=255) ggCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg > 1:100285/1‑66 (MQ=255) ggCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg > 2:176679/1‑66 (MQ=255) ggCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg < 1:176679/66‑1 (MQ=255) gCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtgtt > 2:1021993/1‑58 (MQ=255) gCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggt < 1:1021993/60‑1 (MQ=255) tGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTg > 1:866391/1‑38 (MQ=255) tGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTg < 2:866391/38‑1 (MQ=255) tGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAt > 2:979601/1‑139 (MQ=18) cGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg > 1:245389/1‑82 (MQ=14) cGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg < 2:245389/82‑1 (MQ=17) cGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc > 2:845337/1‑88 (MQ=18) | CTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAT > NC_000913/1287086‑1287343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |