| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 531,656 | G→A | A189V (GCG→GTG) | allS ← | allD operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 531,656 | 0 | G | A | 96.7% | 86.8 / ‑4.0 | 30 | A189V (GCG→GTG) | allS | allD operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (14/15); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CGCCAGATGGGCGGCGATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGT > NC_000913/531520‑531768 | cgccAGATGGGCGGCGATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACt > 2:821624/1‑139 (MQ=255) gggCGGCGATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAg < 1:562608/139‑1 (MQ=255) ggcggcGATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGc < 1:736899/139‑1 (MQ=255) ggcgATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGc < 1:981528/139‑1 (MQ=255) gcgATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGct > 1:626121/1‑139 (MQ=255) aataatCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATcc < 2:808671/139‑1 (MQ=255) tctcTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCcgccgc > 1:295919/1‑139 (MQ=255) ttttGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGa > 2:27377/1‑139 (MQ=255) ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGTGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc < 2:789971/122‑1 (MQ=255) ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGTGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc > 1:789971/1‑122 (MQ=255) ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGcc > 2:390163/1‑139 (MQ=255) ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGcc > 1:704006/1‑139 (MQ=255) ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCAGCAACTGCACTTCTGTTAGCGACTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGcc > 1:640047/1‑139 (MQ=255) cAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCAt < 2:626121/139‑1 (MQ=255) cGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCa < 1:775154/139‑1 (MQ=255) aCGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTa < 2:295919/139‑1 (MQ=255) cgcgTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaa < 2:704098/139‑1 (MQ=255) cgcgTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAc < 2:474836/130‑1 (MQ=255) cgcgTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAc > 1:474836/1‑130 (MQ=255) cgcgTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaa < 1:27377/139‑1 (MQ=255) gTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTaa < 1:821624/139‑1 (MQ=255) cGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAg < 1:849804/56‑1 (MQ=255) cGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAg > 2:849804/1‑56 (MQ=255) gggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc < 1:929962/113‑1 (MQ=255) gggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc > 2:929962/1‑113 (MQ=255) cTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc < 2:1051692/108‑1 (MQ=255) cTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc > 1:1051692/1‑108 (MQ=255) tGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAg < 2:432170/104‑1 (MQ=255) tGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAg > 1:432170/1‑104 (MQ=255) tGACCGCCGGAAAGCGCCGCAACTGCACTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGt > 2:516608/1‑139 (MQ=255) | CGCCAGATGGGCGGCGATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGT > NC_000913/531520‑531768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |