Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	607,988	G→A	*51* (TAG→TAA)	hokE →	toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	607,988	0	G	A	100.0%	188.6 / NA	58	*51* (TAG→TAA)	hokE	toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/33);  total (25/33)

CCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCAT  >  NC_000913/607853‑608106                                                                                                                                        |                                                                                                                       ccTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCg                                                                                                                     <  1:521786/139‑1 (MQ=255)                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                              <  1:531930/124‑1 (MQ=255)                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                              >  2:531930/1‑124 (MQ=255)                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCAt                                                                                                <  2:416071/138‑1 (MQ=255)                       tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCAt                                                                                                >  1:416071/1‑138 (MQ=255)                         tttAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAg                                                                                             >  2:117391/1‑139 (MQ=255)                             aCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGGGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACAGTTGTGCGGGGAGTAATCCCATAAGctct                                                                                         <  2:622027/139‑1 (MQ=255)                              cGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTa                                                                                        >  2:639796/1‑139 (MQ=255)                                 tGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACt                                                                                     >  1:1011378/1‑139 (MQ=255)                                   cTGGGATTTGCGCTTCTGGTCGGAGACTCGCTGTATGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCCCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                   <  1:688356/139‑1 (MQ=255)                                   cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                   <  1:858132/139‑1 (MQ=255)                                   cTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                   <  1:537473/139‑1 (MQ=255)                                       gATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                               <  1:13723/139‑1 (MQ=255)                                       gATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                               <  1:117391/139‑1 (MQ=255)                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGa                                                                                                          >  1:262106/1‑108 (MQ=255)                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGa                                                                                                          <  2:262106/108‑1 (MQ=255)                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                         <  1:464370/125‑1 (MQ=255)                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCt                                                                                         >  2:464370/1‑125 (MQ=255)                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgt                                                                           >  2:595274/1‑139 (MQ=255)                                           tACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgt                                                                           >  1:950906/1‑139 (MQ=255)                                                ttCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTAGATTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                      >  2:626800/1‑139 (MQ=255)                                                    ggTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACg                                                                  <  1:427254/139‑1 (MQ=255)                                                     gTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc                                                                 <  1:639796/139‑1 (MQ=255)                                                      tCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc                                                                <  2:1048288/139‑1 (MQ=255)                                                      tCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc                                                                <  2:950906/139‑1 (MQ=255)                                                          agaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGat                                                            >  1:70850/1‑139 (MQ=255)                                                                gCTGTGTGAGTTTACGGTGAAGGATCGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                      <  1:898302/123‑1 (MQ=255)                                                                gCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                                                      >  2:898302/1‑123 (MQ=255)                                                                       gAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTg                                               <  2:1011378/139‑1 (MQ=255)                                                                            tACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGc                                                                                                               >  1:583317/1‑70 (MQ=255)                                                                            tACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGc                                                                                                               <  2:583317/70‑1 (MQ=255)                                                                              cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                           >  1:141916/1‑88 (MQ=255)                                                                              cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAgcg                                                                                           <  2:141916/88‑1 (MQ=255)                                                                              cGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGa                                        >  2:391269/1‑139 (MQ=255)                                                                                gTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                              <  1:36504/67‑1 (MQ=255)                                                                                gTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                              <  1:88623/67‑1 (MQ=255)                                                                                gTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                              >  2:36504/1‑67 (MQ=255)                                                                                gTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCg                                                                                                              >  2:88623/1‑67 (MQ=255)                                                                                gTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                                      <  2:70850/139‑1 (MQ=255)                                                                                    aGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                  <  1:626800/139‑1 (MQ=255)                                                                                        aCGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa                              >  1:646337/1‑139 (MQ=255)                                                                                                    gTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                               >  1:805/1‑78 (MQ=255)                                                                                                    gTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                               <  2:805/78‑1 (MQ=255)                                                                                                     tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtgtg                                                                                                                <  2:182176/44‑1 (MQ=255)                                                                                                     tttAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTtgtg                                                                                                                >  1:182176/1‑44 (MQ=255)                                                                                                        aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGt                                                                                                                    >  2:40771/1‑37 (MQ=255)                                                                                                        aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGt                                                                                                                    <  1:40771/37‑1 (MQ=255)                                                                                                        aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                   <  1:1061026/70‑1 (MQ=255)                                                                                                        aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTa                                                                                   >  2:1061026/1‑70 (MQ=255)                                                                                                          ggCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                               <  1:626244/72‑1 (MQ=255)                                                                                                          ggCTCTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTCAggg                                                                               >  2:626244/1‑72 (MQ=37)                                                                                                           gCTGTTCTCGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACCGGTCAGCACtt           <  2:622565/139‑1 (MQ=255)                                                                                                                tctcGCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTATGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc      <  2:911467/139‑1 (MQ=255)                                                                                                                    gCTTACGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCat  <  1:391269/139‑1 (MQ=255)                                                                                                                         cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtg                                                                            >  2:58518/1‑60 (MQ=255)                                                                                                                         cGAACCGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtg                                                                            <  1:58518/60‑1 (MQ=255)                                                                                                                              cGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                               <  2:583516/52‑1 (MQ=255)                                                                                                                              cGAAGAAGTAACCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAggg                                                                               >  1:583516/1‑52 (MQ=255)                                                                                                                                        |                                                                                                                       CCTTGCGGCAGTCATAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCAT  >  NC_000913/607853‑608106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑