Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	616,422	A→G	S756G (AGC→GGC)	entF →	enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	616,422	0	A	G	100.0%	62.6 / NA	19	S756G (AGC→GGC)	entF	enterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

AGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616327‑616556                                                                                                |                                                                                                                                       aGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCgg                                                                                             >  2:644871/1‑139 (MQ=255)                                                 cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATc                                                                      >  2:664203/1‑114 (MQ=255)                                                 cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATc                                                                      <  1:664203/114‑1 (MQ=255)                                                                tataTGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    >  2:395119/1‑69 (MQ=255)                                                                tataTGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    <  1:395119/69‑1 (MQ=255)                                                                  tatGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGAGTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGc                            >  2:1037842/1‑139 (MQ=255)                                                                       cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCg                                                >  2:732179/1‑114 (MQ=255)                                                                       cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCg                                                <  1:732179/114‑1 (MQ=255)                                                                       cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCg                       >  1:496440/1‑139 (MQ=255)                                                                       cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCAC‑GGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:118991/1‑139 (MQ=255)                                                                        ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  1:57145/1‑139 (MQ=255)                                                                        ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:1065319/1‑139 (MQ=255)                                                                          gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTg                    <  2:496440/139‑1 (MQ=255)                                                                          gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTg                    >  1:309432/1‑139 (MQ=255)                                                                            cGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTgcc                  >  1:1057777/1‑139 (MQ=255)                                                                               aaGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTgccgcc               <  1:1065319/139‑1 (MQ=255)                                                                                   ggcggTAGATGTCGGCTGGTATCCGGCTGTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGgt           <  1:644871/139‑1 (MQ=255)                                                                                           aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  1:322031/139‑1 (MQ=255)                                                                                            tGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGt  <  1:567217/139‑1 (MQ=255)                                                                                                |                                                                                                                                       AGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616327‑616556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑