| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 668,430 | G→A | R96C (CGC→TGC) | rlmH ← | 23S rRNA m(3)Psi1915 pseudouridine methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 668,430 | 0 | G | A | 87.9% | 75.7 / 4.2 | 33 | R96C (CGC→TGC) | rlmH | 23S rRNA m(3)Psi1915 pseudouridine methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (14/15); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
TCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCGACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAACATCTGCTCACCCTCTTTGTCGAGTA > NC_000913/668303‑668561 | tctGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCt > 1:636326/1‑139 (MQ=255) cAGCACGCGAACCAACGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACACGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGt > 1:652726/1‑139 (MQ=255) gCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTc > 2:926719/1‑139 (MQ=255) aaCCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCa < 2:795654/139‑1 (MQ=255) gggggAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTg < 2:584638/139‑1 (MQ=255) aGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCGACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTgcggcg < 2:721804/139‑1 (MQ=255) aGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTgcggcg < 2:652726/139‑1 (MQ=255) aGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTgcggcg < 2:563239/139‑1 (MQ=255) gTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTgcggcg < 2:831544/136‑1 (MQ=255) gTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTgcggcg > 1:831544/1‑136 (MQ=255) gcgcCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCGACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCa > 1:228306/1‑139 (MQ=255) aGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGc < 1:12560/139‑1 (MQ=255) aGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGc < 1:658647/139‑1 (MQ=255) aCCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTgg > 2:1015086/1‑139 (MQ=255) gCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCGACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAata < 2:228306/139‑1 (MQ=255) tCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAggg < 1:1015086/139‑1 (MQ=255) gccgccGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGa < 2:409721/139‑1 (MQ=255) ccgccgCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCGACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGAc > 1:1041045/1‑139 (MQ=255) ccgccgCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTcc > 2:416169/1‑75 (MQ=255) ccgccgCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTcc < 1:416169/75‑1 (MQ=255) ccgccgCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCt > 2:15974/1‑95 (MQ=255) ccgccgCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCt < 1:15974/95‑1 (MQ=255) cGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGc > 1:982775/1‑97 (MQ=255) cGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGc < 2:982775/97‑1 (MQ=255) cccTTCAGGCCCGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGgccgcc < 1:926719/139‑1 (MQ=255) cGCCAATCAGTAGACTGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAACATCTGCTc > 1:67631/1‑139 (MQ=255) tGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGc > 2:604089/1‑65 (MQ=255) tGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGc < 1:604089/65‑1 (MQ=255) tGACGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAACATCTGCTCACCCTCTTTGTCGAg > 2:273303/1‑139 (MQ=255) aCGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAACATCTGCTCACCCTCTTTGTCGAGTa > 2:381294/1‑139 (MQ=255) aCGTCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAACATCTGCTCACCCTCTTTGTCGAGTa > 1:85432/1‑139 (MQ=255) tCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAAc > 2:495263/1‑111 (MQ=255) tCGCAACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAAc < 1:495263/111‑1 (MQ=255) | TCTGCGACCAGCACGCGAACCAGCGGATGGGGGAGGGTAAGCGCCGACAGCGACCAGCTCTGCTCAGCCGCCGCTTTACAGGCAGGCGACAACCCTTCAGGCCCGCCAATCAGTAGACTGACGTCGCGACCATCCAGCTTCCAGCGTTCCAGCTCAGCGGCTAACTGCGGCGTATCCCAGGGCTTGCCTGGAATATCGAGGGTGACAATGCGGTTTTTGCCTGCGGCCGCCAACATCTGCTCACCCTCTTTGTCGAGTA > NC_000913/668303‑668561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |