| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 66,447 | T→C | E35G (GAG→GGG) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 66,447 | 0 | T | C | 97.8% | 154.6 / ‑5.8 | 46 | E35G (GAG→GGG) | araD | L‑ribulose‑5‑phosphate 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (24/21); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTT > NC_000913/66333‑66582 | ggCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGAGCCCGCGATCAACGGCGCTGACGTTGc > 1:98561/1‑139 (MQ=255) gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc < 1:1068553/139‑1 (MQ=255) gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc < 1:126891/139‑1 (MQ=255) gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc < 2:97958/139‑1 (MQ=255) tttCGTACCTTCAACCACTTCACCGGTTCCGATGATAACCACGACCATATCGTCAGCGGTCCTGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCAt > 2:694577/1‑139 (MQ=255) tACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg < 2:966740/139‑1 (MQ=255) aaCCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGAcc > 1:516202/1‑139 (MQ=255) cTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACg < 2:456968/113‑1 (MQ=255) cTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACg > 1:456968/1‑113 (MQ=255) ttCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc < 1:607406/111‑1 (MQ=255) ttCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc > 2:607406/1‑111 (MQ=255) cACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAAc < 2:506692/100‑1 (MQ=255) cACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAAc > 1:506692/1‑100 (MQ=255) tAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAg > 1:762270/1‑139 (MQ=255) aaCCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAgg > 1:973453/1‑139 (MQ=255) aCGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTgg < 2:762270/139‑1 (MQ=255) gACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGTAGCGCCAGGTTGGCt > 2:181969/1‑139 (MQ=255) gACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg > 1:409816/1‑136 (MQ=255) gACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg < 2:409816/136‑1 (MQ=255) gACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCt > 2:781934/1‑139 (MQ=255) aCCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg < 2:237875/135‑1 (MQ=255) aCCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg > 1:237875/1‑135 (MQ=255) aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcgc > 1:714603/1‑71 (MQ=255) aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcgc < 2:714603/71‑1 (MQ=255) aGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTgg > 1:688044/1‑139 (MQ=255) cGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt > 1:783536/1‑75 (MQ=255) cGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt < 2:783536/75‑1 (MQ=255) cATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACAAGGTTGTGTTTTGGCAGCGCCAGGTTGGCT‑‑TAATACCTGGCGTTTgag < 2:98561/139‑1 (MQ=255) aTGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTga < 2:973453/139‑1 (MQ=255) tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag < 2:688044/139‑1 (MQ=255) gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGAtcttct < 2:868721/139‑1 (MQ=255) aTCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACga < 2:671373/59‑2 (MQ=255) aTCGACGCCGGAAGGTTGGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt > 1:671373/1‑59 (MQ=39) cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcgc < 1:331717/45‑1 (MQ=255) cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcgc > 2:331717/1‑45 (MQ=255) aaGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTcc > 2:445819/1‑139 (MQ=255) ggTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCtt < 2:467231/139‑1 (MQ=255) gATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg > 1:644040/1‑54 (MQ=255) gATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg < 2:644040/54‑1 (MQ=255) aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATg > 2:884466/1‑139 (MQ=255) cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg > 1:944811/1‑43 (MQ=255) cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg < 2:944811/43‑1 (MQ=255) ccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTg > 2:417869/1‑139 (MQ=255) cgcgCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTgt > 1:27839/1‑138 (MQ=255) cgcCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTaa > 2:27123/1‑104 (MQ=255) cgcCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCtt > 1:100152/1‑139 (MQ=255) | GGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTT > NC_000913/66333‑66582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |