Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 684,616 T→C I214V (ATT→GTT)  gltL ← glutamate/aspartate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913684,6160TC100.0% 72.0 / NA 23I214V (ATT→GTT) gltLglutamate/aspartate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

GATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATC  >  NC_000913/684484‑684746
                                                                                                                                    |                                                                                                                                  
gATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTAcc                                                                                                                              <  2:115800/139‑1 (MQ=255)
                                  gcgcCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGATAACTTTACCCTc                                                                                                                           <  1:1033086/108‑1 (MQ=255)
                                  gcgcCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGATAACTTTACCCTc                                                                                                                           >  2:1033086/1‑108 (MQ=255)
                                     ccAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTc                                                                                                                           >  2:723070/1‑105 (MQ=255)
                                     ccAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTc                                                                                                                           <  1:723070/105‑1 (MQ=255)
                                                       gATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTgg                                                                       >  1:743747/1‑139 (MQ=255)
                                                         ttttCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGt                                                                     <  1:26460/139‑1 (MQ=255)
                                                              gcgAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcacca                                                                >  1:415375/1‑139 (MQ=255)
                                                                                      gATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCttcgttcg                                        <  2:866079/139‑1 (MQ=255)
                                                                                          tCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAg                                    <  2:803417/139‑1 (MQ=255)
                                                                                             gATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTc                                 >  2:546004/1‑139 (MQ=255)
                                                                                                 atcGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTccacc                             >  2:182039/1‑139 (MQ=255)
                                                                                                          aGCGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTcc                    >  1:949790/1‑139 (MQ=255)
                                                                                                            cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                                  <  1:471707/91‑1 (MQ=255)
                                                                                                            cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTcac                                                                  >  2:471707/1‑91 (MQ=255)
                                                                                                            cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg                  >  1:741337/1‑139 (MQ=255)
                                                                                                            cGTCTTTCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAg                  >  1:406638/1‑139 (MQ=255)
                                                                                                                  tCGGCGAGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTc            >  2:685397/1‑139 (MQ=255)
                                                                                                                        aGTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGat      <  2:1066024/139‑1 (MQ=255)
                                                                                                                         gTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatc     <  2:741337/139‑1 (MQ=255)
                                                                                                                         gTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatc     <  1:546004/139‑1 (MQ=255)
                                                                                                                         gTCTTCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatc     >  2:948959/1‑139 (MQ=255)
                                                                                                                            ttCGACAACTTTACCCTCGTCCATAAAGATCACCCGATTCGCCCCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGatcatc  >  2:316544/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                  
GATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATC  >  NC_000913/684484‑684746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: