Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	875,327	G→A	*783* (TAG→TAA)	yliE →	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	875,327	0	G	A	100.0%	106.9 / NA	35	*783* (TAG→TAA)	yliE	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/18);  total (17/18)

AAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAA  >  NC_000913/875197‑875451                                                                                                                                   |                                                                                                                             aaGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTa                                                                                                                      >  2:533454/1‑139 (MQ=255)       tAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa                                                                                                                <  1:850210/139‑1 (MQ=255)              cGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCaa                                                                                                         >  1:630940/1‑139 (MQ=255)                aaGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATa                                                                                                       >  1:516395/1‑139 (MQ=255)                      ttGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                 <  2:630940/139‑1 (MQ=255)                       tGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGt                                                                                                >  2:943786/1‑139 (MQ=255)                                               aTGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAATGAGTAATTTACGt                                                                                                                       <  1:630748/92‑1 (MQ=255)                                               aTGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                       >  2:630748/1‑92 (MQ=255)                                                                   aTCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTg                                                    >  1:41491/1‑139 (MQ=255)                                                                    tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                 >  1:268349/1‑93 (MQ=255)                                                                    tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                 <  2:268349/93‑1 (MQ=255)                                                                            aGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCaa                                                                                                         >  1:890803/1‑77 (MQ=255)                                                                            aGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCaa                                                                                                         <  2:890803/77‑1 (MQ=255)                                                                              aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGAt                                          <  2:730034/138‑1 (MQ=255)                                                                              aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGAt                                          >  1:730034/1‑138 (MQ=255)                                                                              aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATc                                         <  2:40745/139‑1 (MQ=255)                                                                                aCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTa                                       >  1:55190/1‑139 (MQ=255)                                                                                aCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTa                                       >  2:6636/1‑139 (MQ=255)                                                                                aCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTa                                       <  2:516395/139‑1 (MQ=255)                                                                                  ccATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                 <  1:900384/79‑1 (MQ=255)                                                                                  ccATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                 >  2:900384/1‑79 (MQ=255)                                                                                    aTGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                 >  2:313532/1‑77 (MQ=255)                                                                                    aTGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                 <  1:313532/77‑1 (MQ=255)                                                                                                gCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATg                                                                 >  2:503435/1‑97 (MQ=255)                                                                                                gCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATg                                                                 <  1:503435/97‑1 (MQ=255)                                                                                                   tGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGaa                    <  1:334638/139‑1 (MQ=255)                                                                                                      gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                       >  2:425848/1‑37 (MQ=255)                                                                                                      gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                       <  1:425848/37‑1 (MQ=255)                                                                                                           aaGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGta            <  1:943786/139‑1 (MQ=255)                                                                                                           aaGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGta            <  1:494288/139‑1 (MQ=255)                                                                                                                gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt                                                                  <  1:192119/80‑1 (MQ=255)                                                                                                                gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt                                                                  >  2:192119/1‑80 (MQ=255)                                                                                                                gAAAATTAGTAAAAGAGTAATTTACGTAAGTCCAGAATCAATAAGTTCGTACTTACAGTCAATCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACAGTCAAATGGTAAAGGAACGGGTGTATAGcc       >  2:694082/1‑139 (MQ=255)                                                                                                                   aaTTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGa    <  1:6636/139‑1 (MQ=255)                                                                                                                     ttAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGaaa  <  1:533454/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                             AAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAA  >  NC_000913/875197‑875451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑