Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	892,869	G→A	*301* (TAG→TAA)	rimK →	ribosomal protein S6 modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	892,869	0	G	A	100.0%	130.2 / NA	41	*301* (TAG→TAA)	rimK	ribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/23);  total (18/23)

GTTGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAA  >  NC_000913/892734‑892998                                                                                                                                        |                                                                                                                                  gTTGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAGCCACCGGTATCGACATCGCGGGTAAAATG‑TCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               <  1:187489/139‑1 (MQ=255)  ttGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               <  2:354038/139‑1 (MQ=255)    ggTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCaa                                                                                                                             <  1:1075950/139‑1 (MQ=255)       gATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                          <  2:476038/139‑1 (MQ=255)          ggAGGTGAATGCGTCGCCGGGGCGGGAAGGAATAGTAAAAACCACCGGTATCGACATCGCGGGTAAAACG‑TTCGCTGAATCGAACGCCACTCAACGACAGAATATGGCCTGGAAACGGGCGGGTAATCGCAATCACAtt                                                                                                                      >  2:187489/1‑139 (MQ=255)           gAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACAtt                                                                                                                      <  2:680614/139‑1 (MQ=255)             ggTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               >  1:693282/1‑128 (MQ=255)             ggTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                          >  1:217256/1‑133 (MQ=255)             ggTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATca                                                                                                                          <  2:217256/133‑1 (MQ=255)             ggTGAATGCGTCGCCGGGACTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               <  2:693282/128‑1 (MQ=255)                 aaTGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGAt                                                                                                                <  1:724229/139‑1 (MQ=255)                                cTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTgc                                                                                                 >  1:435816/1‑139 (MQ=255)                                    aaggaaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCtt                                                                                             <  2:435816/139‑1 (MQ=255)                                    aaggaaTAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCtt                                                                                             <  1:404850/139‑1 (MQ=255)                                               aaaaCCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGc                                                                                  <  1:54375/139‑1 (MQ=255)                                                               aCATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTa                                                                  >  1:467994/1‑139 (MQ=255)                                                                   cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTTTCGa                                                              >  2:995079/1‑139 (MQ=255)                                                                   cgcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGa                                                              >  2:92533/1‑139 (MQ=255)                                                                    gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            >  1:389149/1‑76 (MQ=255)                                                                    gcgGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAAt                                                                                                                            <  2:389149/76‑1 (MQ=255)                                                                               gATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTAc                                                  <  2:860461/139‑1 (MQ=255)                                                                                        gATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                         >  2:874209/1‑139 (MQ=255)                                                                                         aTCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGa                                        <  1:457901/139‑1 (MQ=255)                                                                                             aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAg                                                                                   <  1:474958/92‑1 (MQ=255)                                                                                             aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAg                                                                                   >  2:474958/1‑92 (MQ=255)                                                                                             aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACaa                                                <  1:897854/127‑1 (MQ=255)                                                                                             aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACaa                                                >  2:897854/1‑127 (MQ=255)                                                                                             aaCGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATc                                    >  1:269252/1‑139 (MQ=255)                                                                                                   aCGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGt                              <  2:269252/139‑1 (MQ=255)                                                                                                    cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               <  1:177118/41‑1 (MQ=255)                                                                                                    cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGc                                                                                                                               >  2:177118/1‑41 (MQ=255)                                                                                                    cGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTc                             >  2:544060/1‑139 (MQ=255)                                                                                                         cGACAGAATATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTcc                        <  1:92533/139‑1 (MQ=255)                                                                                                               aaTATTGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCt                  >  1:232699/1‑139 (MQ=255)                                                                                                                   ttGCCTGAAAACGGGTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGAt              >  2:780180/1‑139 (MQ=255)                                                                                                                               gggTGGTTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCaa  <  2:290635/139‑1 (MQ=255)                                                                                                                                ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                            >  2:53435/1‑64 (MQ=255)                                                                                                                                ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGcc                                                                            <  1:53435/64‑1 (MQ=255)                                                                                                                                ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                         <  2:106025/99‑1 (MQ=255)                                                                                                                                ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATg                                         >  1:106025/1‑99 (MQ=255)                                                                                                                                ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGa                                        >  1:424927/1‑100 (MQ=255)                                                                                                                                ggtggtTAATCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGa                                        <  2:424927/100‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  GTTGGTGATGGAGGTGAATGCGTCGCCGGGGCTGGAAGGAATAGAAAAAACCACCGGTATCGACATCGCGGGTAAAATGATCCGCTGGATCGAACGCCACGCTACGACAGAATATTGCCTGAAAACGGGTGGTTAGTCGCAATCACATTACTGATCATGGTTTTGCCTGCGCTTTTTGCGTAAGCTGTGCCGGTCTTTTTATCGAAAGAGGTTGTACAAAATTATGACATCGCTGGTCGTTCCTGGTCTGGATACGCTGCGTCAA  >  NC_000913/892734‑892998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑