| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,019,150 | C→T | E302K (GAA→AAA) | ompA ← | outer membrane protein A (3a,II*,G,d) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,019,150 | 0 | C | T | 87.0% | 103.5 / 9.0 | 46 | E302K (GAA→AAA) | ompA | outer membrane protein A (3a,II*,G,d) |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (19/21); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
CGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACC > NC_000913/1019022‑1019279 | cGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAAGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCa > 2:762000/1‑139 (MQ=255) ggCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCAc < 1:762000/139‑1 (MQ=255) aGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCGCGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCg < 2:628016/139‑1 (MQ=255) tttGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCt > 1:222353/1‑139 (MQ=255) cTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGa < 2:522233/138‑1 (MQ=255) cTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGa > 1:522233/1‑138 (MQ=255) cTTTAACTTCGATATCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCTCAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGAt > 2:713758/1‑139 (MQ=255) ttAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATAc > 2:973249/1‑139 (MQ=255) cGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATAc > 1:62084/1‑132 (MQ=255) cGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATAc < 2:62084/132‑1 (MQ=255) gATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGga > 1:1027606/1‑138 (MQ=255) gATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGga < 2:1027606/138‑1 (MQ=255) ctctACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATc < 2:871619/139‑1 (MQ=255) tACGCGACGATCCGGAGCCATGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTTCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAgg < 1:796057/139‑1 (MQ=255) cgacgaTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAAt < 1:973249/139‑1 (MQ=255) cgaTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaa > 2:968579/1‑139 (MQ=255) gCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACt < 1:713758/139‑1 (MQ=255) ggCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATca > 2:929076/1‑125 (MQ=255) ggCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATca < 1:929076/125‑1 (MQ=255) gCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCa > 2:480591/1‑139 (MQ=255) aCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTgg > 1:55808/1‑92 (MQ=255) aCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTgg < 2:55808/92‑1 (MQ=255) tGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGAAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGt > 2:716043/1‑139 (MQ=255) tGTTTCACGTTGTCACAGCTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGt > 2:596567/1‑139 (MQ=255) tCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:844962/139‑1 (MQ=255) ttGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGt < 1:968579/139‑1 (MQ=255) tCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTg > 2:1074510/1‑46 (MQ=255) tCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTg < 1:1074510/46‑1 (MQ=255) tCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa > 1:324678/1‑131 (MQ=255) tCACAGGTGTTGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 2:324678/131‑1 (MQ=255) tGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCa < 2:790459/95‑1 (MQ=255) tGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCa > 1:790459/1‑95 (MQ=255) tGCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGcg > 1:499263/1‑139 (MQ=255) tGCCAGTAACCGGGTTGGATTTGCACATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGAt > 2:728948/1‑136 (MQ=255) tGCCAGTAACCGGATTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGATTGTAAGCGTCAGAACCGAt < 1:728948/136‑1 (MQ=255) gCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGcgg > 1:367097/1‑139 (MQ=255) gCCAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGcgg > 2:452660/1‑139 (MQ=255) ccAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAgg < 2:478921/72‑1 (MQ=255) ccAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAgg > 1:478921/1‑72 (MQ=255) ccAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGcggt < 2:739171/139‑1 (MQ=255) ccAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGcggt < 1:452660/139‑1 (MQ=255) ccAGTAACCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGcggt < 2:499263/139‑1 (MQ=255) ccAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGcggt < 1:596567/139‑1 (MQ=255) aCCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGa > 1:925519/1‑98 (MQ=255) aCCGGGTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGa < 2:925519/98‑1 (MQ=255) ggTTGGATTTGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAAcc < 2:222353/139‑1 (MQ=255) | CGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACC > NC_000913/1019022‑1019279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |