Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,073,732 C→T A94T (GCG→ACG)  rutA ← pyrimidine oxygenase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,073,7320CT100.0% 72.4 / NA 24A94T (GCG→ACG) rutApyrimidine oxygenase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/10);  total (14/10)

CAGCCAGTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAA  >  NC_000913/1073596‑1073857
                                                                                                                                        |                                                                                                                             
cagccagTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCa                                                                                                                             >  2:200745/1‑139 (MQ=255)
      gTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCg                                                                                                                       >  1:602961/1‑139 (MQ=255)
      gTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCg                                                                                                                       >  2:130206/1‑139 (MQ=255)
            aGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCa                                                                                                                 <  1:363954/139‑1 (MQ=255)
                                          gAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGa                                                                                   >  1:485502/1‑139 (MQ=255)
                                            gTCGATGGGTGCGGTCATACGGGCAACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCAAGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAAc                                                                                 >  2:520192/1‑139 (MQ=255)
                                                 tGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGt                                                                            >  2:656139/1‑139 (MQ=255)
                                                       cGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTgcc                                                                      >  2:1023241/1‑139 (MQ=255)
                                                         gCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTgccgc                                                                    <  2:602961/139‑1 (MQ=255)
                                                             tACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCaaa                                                                >  1:676223/1‑139 (MQ=255)
                                                                 ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                            <  1:656139/139‑1 (MQ=255)
                                                                        aTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGtt                                                     <  2:714644/139‑1 (MQ=255)
                                                                                                 cagcagTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                            <  2:882468/107‑1 (MQ=255)
                                                                                                 cagcagTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                            >  2:521901/1‑107 (MQ=255)
                                                                                                 cagcagTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                            >  1:882468/1‑107 (MQ=255)
                                                                                                 cagcagTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                            <  1:521901/107‑1 (MQ=255)
                                                                                                              aaTCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcga                                   >  2:281946/1‑119 (MQ=255)
                                                                                                              aaTCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcga                                   <  1:281946/119‑1 (MQ=255)
                                                                                                              aaTCTGAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTcc               <  2:676223/139‑1 (MQ=255)
                                                                                                                   gAATGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCttt          >  2:409550/1‑139 (MQ=255)
                                                                                                                    aaTGCGCGAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCtttt         >  1:244261/1‑139 (MQ=255)
                                                                                                                        cgcgAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGc                                                         >  2:24233/1‑87 (MQ=255)
                                                                                                                        cgcgAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGc                                                         <  1:24233/87‑1 (MQ=255)
                                                                                                                           gAGGTCACGGCCGTCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa  <  2:244261/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                             
CAGCCAGTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAA  >  NC_000913/1073596‑1073857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: