Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,200,881	G→A	A51V (GCC→GTC)	xisE ←	e14 prophage, putative excisionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,200,881	0	G	A	100.0%	199.9 / NA	63	A51V (GCC→GTC)	xisE	e14 prophage, putative excisionase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/30);  total (33/30)

TAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAG  >  NC_000913/1200751‑1201014                                                                                                                                   |                                                                                                                                      taAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCa                                                                                                                               >  1:125838/1‑139 (MQ=255)    aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGc                                                                                                                            >  2:1044507/1‑139 (MQ=255)    aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGc                                                                                                                            >  1:456711/1‑139 (MQ=255)        gATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTc                                                                                                                        >  1:327079/1‑139 (MQ=255)         atatAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCg                                                                                                                       <  1:748791/139‑1 (MQ=255)                   gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACg                                                                                                                             >  2:388859/1‑123 (MQ=255)                   gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACg                                                                                                                             <  1:388859/123‑1 (MQ=255)                   gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCgg                                                                                                             >  1:108430/1‑139 (MQ=255)                          aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACg                                                                                                                                     <  2:1062030/108‑1 (MQ=255)                          aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGtt                                                                                                      >  1:1034284/1‑139 (MQ=255)                             cATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTATCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCa                                                                                                   >  2:926184/1‑139 (MQ=255)                                tattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTAACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATgg                                                                                                >  2:680198/1‑139 (MQ=255)                                 attCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGc                                                                                               <  2:30518/139‑1 (MQ=255)                                       aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                         >  2:701848/1‑139 (MQ=255)                                       aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                         >  2:909736/1‑139 (MQ=255)                                            gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                         <  2:601867/134‑1 (MQ=255)                                            gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                         >  1:601867/1‑134 (MQ=255)                                            gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    >  1:796218/1‑139 (MQ=255)                                            gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    >  2:29111/1‑139 (MQ=255)                                             tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    >  1:413804/1‑138 (MQ=255)                                             tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    <  2:413804/138‑1 (MQ=255)                                                 gCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaataaa                                                                               <  2:456711/139‑1 (MQ=255)                                                      cATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTg                                                                          <  2:529229/139‑1 (MQ=255)                                                      cATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTg                                                                          <  2:125838/139‑1 (MQ=255)                                                       aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGc                                                                         >  1:251208/1‑139 (MQ=255)                                                        ttACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                        <  1:680198/139‑1 (MQ=255)                                                                     gTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                           >  2:192115/1‑139 (MQ=255)                                                                       tGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCa                                                         <  1:29111/139‑1 (MQ=255)                                                                              gTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                  <  1:1044507/139‑1 (MQ=255)                                                                              gTAATGAGTCATTTTTCTTAATTACAGGTGTTACCAACCCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCATTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCACAACGGCGCAATGTGGa                                                  <  2:488501/139‑1 (MQ=255)                                                                               tAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGac                                                 <  1:344452/139‑1 (MQ=255)                                                                                aaTGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGaca                                                <  2:1034284/139‑1 (MQ=255)                                                                                       cATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                         <  2:108430/139‑1 (MQ=255)                                                                                         tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                  <  1:926540/128‑1 (MQ=255)                                                                                         tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                  >  1:210853/1‑128 (MQ=255)                                                                                         tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                  >  2:926540/1‑128 (MQ=255)                                                                                         tttttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                  <  2:210853/128‑1 (MQ=255)                                                                                              cTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTa                                  >  1:304257/1‑139 (MQ=255)                                                                                                    tACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAt                                    >  2:935711/1‑131 (MQ=255)                                                                                                    tACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAt                                    <  1:935711/131‑1 (MQ=255)                                                                                                      cAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTa                          >  2:43085/1‑139 (MQ=255)                                                                                                           gtTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTAATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAttttt                     >  1:710891/1‑139 (MQ=255)                                                                                                            ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    <  1:663015/75‑1 (MQ=255)                                                                                                            ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    >  2:663015/1‑75 (MQ=255)                                                                                                            ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                            >  1:622953/1‑99 (MQ=255)                                                                                                            ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                            <  2:622953/99‑1 (MQ=255)                                                                                                              aCCAACCCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaataaa                                                                               >  1:544150/1‑78 (MQ=255)                                                                                                              aCCAACCCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaataaa                                                                               <  2:544150/78‑1 (MQ=255)                                                                                                                cAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                     <  1:1054859/86‑1 (MQ=255)                                                                                                                cAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                     >  2:1054859/1‑86 (MQ=255)                                                                                                                cAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagca                <  2:327079/139‑1 (MQ=255)                                                                                                                   cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                            >  1:757056/1‑92 (MQ=255)                                                                                                                   cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                            <  2:757056/92‑1 (MQ=255)                                                                                                                   cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGcc             <  1:43085/139‑1 (MQ=255)                                                                                                                         aactaactCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATCACTTCTGTATTTTTCAGCAGCCCAttct       >  1:645167/1‑139 (MQ=255)                                                                                                                          actaactCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                        >  1:95688/1‑73 (MQ=255)                                                                                                                          actaactCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                        <  2:95688/73‑1 (MQ=255)                                                                                                                              actCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                           <  2:742462/82‑1 (MQ=255)                                                                                                                              actCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                           >  1:742462/1‑82 (MQ=255)                                                                                                                              actCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag  >  2:546116/1‑139 (MQ=255)                                                                                                                                tCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                           <  2:74499/80‑1 (MQ=255)                                                                                                                                tCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                         <  1:268626/98‑1 (MQ=255)                                                                                                                                tCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                         >  2:268626/1‑98 (MQ=255)                                                                                                                                   |                                                                                                                                      TAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAG  >  NC_000913/1200751‑1201014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑