Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,248,410 A→G A235A (GCT→GCC dhaM ← putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,248,4100AG100.0% 170.2 / NA 48A235A (GCT→GCCdhaMputative dihydroxyacetone‑specific PTS enzymes: HPr, EI components
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/25);  total (23/25)

CCACGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAC  >  NC_000913/1248280‑1248516
                                                                                                                                  |                                                                                                          
ccACGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTg                                                                                                    <  2:964904/139‑1 (MQ=255)
  aCGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTAGTTGATAATAAAAGCCTTTGCCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACTAAAGTTATCGTCGGCCAGCTGAc                                                                                                  >  2:708591/1‑139 (MQ=255)
             tGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATc                                                                                       <  2:913626/139‑1 (MQ=255)
                         tACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCa                                                                           >  2:778529/1‑139 (MQ=255)
                             gTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCtt                                                                       >  1:741877/1‑139 (MQ=255)
                                                aataaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAg                                                    <  2:637002/139‑1 (MQ=255)
                                                        gCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAg                                              <  2:559138/137‑1 (MQ=255)
                                                        gCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAg                                              >  1:559138/1‑137 (MQ=255)
                                                        gCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCg                                            <  1:859372/139‑1 (MQ=255)
                                                          tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGAc                                                                                                  <  1:88571/83‑1 (MQ=255)
                                                          tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGAc                                                                                                  >  2:88571/1‑83 (MQ=255)
                                                          tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGAGACTTCCTCAGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTa                                          >  2:661151/1‑139 (MQ=255)
                                                           ttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt                                         >  2:557812/1‑139 (MQ=255)
                                                           ttACCCGAAACAGGCGGAACGAGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt                                         >  2:1038692/1‑139 (MQ=255)
                                                                   aaCAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAg                                 >  1:292478/1‑139 (MQ=255)
                                                                       ggCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGaa                             <  1:610115/139‑1 (MQ=255)
                                                                        gCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAAc                            >  1:665505/1‑139 (MQ=255)
                                                                         cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGaa                                                                                              <  2:681247/72‑1 (MQ=255)
                                                                         cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGaa                                                                                              >  1:681247/1‑72 (MQ=255)
                                                                         cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTc                                                                            >  2:490676/1‑90 (MQ=255)
                                                                         cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTc                                                                            <  1:490676/90‑1 (MQ=255)
                                                                             aCGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGc                                                                                  >  1:445952/1‑80 (MQ=255)
                                                                             aCGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGc                                                                                  <  2:445952/80‑1 (MQ=255)
                                                                                ggACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagta                    <  1:843201/139‑1 (MQ=255)
                                                                                       gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt             <  1:557812/139‑1 (MQ=255)
                                                                                       gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt             <  1:845970/139‑1 (MQ=255)
                                                                                       gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt             <  1:933721/139‑1 (MQ=255)
                                                                                       gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt             <  2:991592/139‑1 (MQ=255)
                                                                                       gagTAGGTGGAGCGACTTCCTCCGATTCACCAAAGTTATCTCCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt             <  2:614394/139‑1 (MQ=255)
                                                                                        agTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg                                                                                            <  2:707551/59‑1 (MQ=255)
                                                                                        agTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg                                                                                            >  1:707551/1‑59 (MQ=255)
                                                                                         gTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCt           <  2:665505/139‑1 (MQ=255)
                                                                                            ggTGGAGCGACTTCCTCAGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCACTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTggc        <  1:661151/139‑2 (MQ=255)
                                                                                             gTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg                                                                                   <  2:984188/63‑1 (MQ=255)
                                                                                             gTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg                                                                                   >  1:984188/1‑63 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTcggccag                                                                                                       >  2:215920/1‑38 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTcggccag                                                                                                       <  1:215920/38‑1 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACgg                                                                                                >  2:487676/1‑45 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACgg                                                                                                <  1:487676/45‑1 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg                                                                                            >  2:448331/1‑49 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg                                                                                            <  1:448331/49‑1 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg                                                                                   <  2:414160/58‑1 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg                                                                                   >  1:414160/1‑58 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAc  >  1:950936/1‑139 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAc  >  1:463334/1‑139 (MQ=255)
                                                                                                  gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAc  >  1:301693/1‑139 (MQ=255)
                                                                                                    gACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCg                                                           >  1:751269/1‑80 (MQ=255)
                                                                                                    gACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCg                                                           <  2:751269/80‑1 (MQ=255)
                                                                                                      cTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt             <  2:121549/124‑1 (MQ=255)
                                                                                                      cTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt             >  1:121549/1‑124 (MQ=255)
                                                                                                                                  |                                                                                                          
CCACGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAC  >  NC_000913/1248280‑1248516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: