Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,248,410 | A→G | A235A (GCT→GCC) | dhaM ← | putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,248,410 | 0 | A | G | 100.0% | 170.2 / NA | 48 | A235A (GCT→GCC) | dhaM | putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/25); total (23/25) |
CCACGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAC > NC_000913/1248280‑1248516 | ccACGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTg < 2:964904/139‑1 (MQ=255) aCGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTAGTTGATAATAAAAGCCTTTGCCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACTAAAGTTATCGTCGGCCAGCTGAc > 2:708591/1‑139 (MQ=255) tGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATc < 2:913626/139‑1 (MQ=255) tACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCa > 2:778529/1‑139 (MQ=255) gTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCtt > 1:741877/1‑139 (MQ=255) aataaAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAg < 2:637002/139‑1 (MQ=255) gCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAg < 2:559138/137‑1 (MQ=255) gCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAg > 1:559138/1‑137 (MQ=255) gCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCg < 1:859372/139‑1 (MQ=255) tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGAc < 1:88571/83‑1 (MQ=255) tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGAc > 2:88571/1‑83 (MQ=255) tttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGAGACTTCCTCAGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTa > 2:661151/1‑139 (MQ=255) ttACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt > 2:557812/1‑139 (MQ=255) ttACCCGAAACAGGCGGAACGAGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTAt > 2:1038692/1‑139 (MQ=255) aaCAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAg > 1:292478/1‑139 (MQ=255) ggCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGaa < 1:610115/139‑1 (MQ=255) gCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAAc > 1:665505/1‑139 (MQ=255) cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGaa < 2:681247/72‑1 (MQ=255) cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGaa > 1:681247/1‑72 (MQ=255) cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTc > 2:490676/1‑90 (MQ=255) cGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTc < 1:490676/90‑1 (MQ=255) aCGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGc > 1:445952/1‑80 (MQ=255) aCGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGc < 2:445952/80‑1 (MQ=255) ggACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTgtagta < 1:843201/139‑1 (MQ=255) gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt < 1:557812/139‑1 (MQ=255) gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt < 1:845970/139‑1 (MQ=255) gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt < 1:933721/139‑1 (MQ=255) gagTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt < 2:991592/139‑1 (MQ=255) gagTAGGTGGAGCGACTTCCTCCGATTCACCAAAGTTATCTCCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt < 2:614394/139‑1 (MQ=255) agTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg < 2:707551/59‑1 (MQ=255) agTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg > 1:707551/1‑59 (MQ=255) gTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCt < 2:665505/139‑1 (MQ=255) ggTGGAGCGACTTCCTCAGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCACTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTggc < 1:661151/139‑2 (MQ=255) gTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg < 2:984188/63‑1 (MQ=255) gTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg > 1:984188/1‑63 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTcggccag > 2:215920/1‑38 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTcggccag < 1:215920/38‑1 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACgg > 2:487676/1‑45 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACgg < 1:487676/45‑1 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg > 2:448331/1‑49 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAg < 1:448331/49‑1 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg < 2:414160/58‑1 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTg > 1:414160/1‑58 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAc > 1:950936/1‑139 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAc > 1:463334/1‑139 (MQ=255) gCGACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAc > 1:301693/1‑139 (MQ=255) gACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCg > 1:751269/1‑80 (MQ=255) gACTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCg < 2:751269/80‑1 (MQ=255) cTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt < 2:121549/124‑1 (MQ=255) cTTCCTCCGTTTCACCAAAGTTATCTTCGGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAAt > 1:121549/1‑124 (MQ=255) | CCACGGTCAGGGTTGATTTTGCCTGTACCGTACATAAAACTGGTTGATAATAAAAGGCTTTACCCGAAACAGGCGGAACGGGACGCAGAGTAGGTGGAGCGACTTCCTCCGTTTCACCAAAGTTATCTTCAGCCAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATAC > NC_000913/1248280‑1248516 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |