Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,528	A→G	I92I (ATT→ATC)	dauA ←	C4‑dicarboxylic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,528	0	A	G	100.0%	98.3 / NA	28	I92I (ATT→ATC)	dauA	C4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

ACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCA  >  NC_000913/1260416‑1260655                                                                                                                 |                                                                                                                                aCTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGTGATAGAGGATTACCACAAATGCCGCAGt                                                                                                             <  1:664684/133‑1 (MQ=255) aCTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt                                                                                                             >  2:664684/1‑133 (MQ=255)     aaTCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa                                                                                                   >  1:530212/1‑139 (MQ=255)                cAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg                                                                                        <  2:446843/139‑1 (MQ=255)                         ccAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGacccacc                                                                               >  1:20731/1‑139 (MQ=255)                                 aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       >  2:331354/1‑139 (MQ=255)                                 aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  1:1033256/139‑1 (MQ=255)                                 aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                                       <  2:1040667/139‑1 (MQ=255)                                        tCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGAc                                                                >  2:781394/1‑139 (MQ=255)                                                  ccccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCa                                                      >  1:226852/1‑139 (MQ=255)                                                  ccccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCa                                                      >  2:225891/1‑139 (MQ=255)                                                      gACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAg                                                  <  2:20731/139‑1 (MQ=255)                                                          gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                                              <  2:136624/139‑1 (MQ=255)                                                           cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCg                                             <  1:61820/139‑1 (MQ=255)                                                           cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCg                                             <  1:331354/139‑1 (MQ=255)                                                                   cAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATc                                                            >  1:643650/1‑116 (MQ=255)                                                                   cAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATc                                                            <  2:643650/116‑1 (MQ=255)                                                                    aaCCAGCAGTCCTGCCAGTCCAAACTGTTGTGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATaaa                                    <  1:577851/139‑1 (MQ=255)                                                                            gTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg                            <  1:781394/139‑1 (MQ=255)                                                                             tCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgg                           <  2:660144/139‑1 (MQ=255)                                                                             tCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgg                           >  2:70560/1‑139 (MQ=255)                                                                               cTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgggg                         <  2:530212/139‑1 (MQ=255)                                                                               cTGCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTgggg                         <  2:821497/139‑1 (MQ=255)                                                                                 gCCAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAGGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTg                       >  1:713088/1‑139 (MQ=255)                                                                                  ccAGTCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGc                      <  2:685437/139‑1 (MQ=255)                                                                                      tCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca                  >  2:907941/1‑139 (MQ=255)                                                                                                tGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCaa        >  2:71698/1‑139 (MQ=255)                                                                                                      acGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCa  <  1:70560/139‑1 (MQ=255)                                                                                                                 |                                                                                                                                ACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCA  >  NC_000913/1260416‑1260655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑